Symbiotic Interaction Enhances the Recovery of Endangered Tree Species in the Fragmented Maulino Forest

Beneficial plant-associated microorganisms, such as fungal endophytes, are key partners that normally improve plant survival under different environmental stresses. It has been shown that microorganisms from extreme environments, like those associated with the roots of Antarctica plants, can be good partners to increase the performance of crop plants and to restore endangered native plants. Nothofagus alessandrii and N. glauca, are among the most endangered species of Chile, restricted to a narrow and/or limited distributional range associated mainly to the Maulino forest in Chile. Here we evaluated the effect of the inoculation with a fungal consortium of root endophytes isolated from the Antarctic host plant Colobanthus quitensis on the ecophysiological performance [photosynthesis, water use efficiency (WUE), and growth] of both endangered tree species. We also, tested how Antarctic root-fungal endophytes could affect the potential distribution of N. alessandrii through niche modeling. Additionally, we conducted a transplant experiment recording plant survival on 2 years in order to validate the model. Lastly, to evaluate if inoculation with Antarctic endophytes has negative impacts on native soil microorganisms, we compared the biodiversity of fungi and bacterial in the rhizospheric soil of transplanted individuals of N. alessandrii inoculated and non-inoculated with fungal endophytes. We found that inoculation with root-endophytes produced significant increases in N. alessandrii and N. glauca photosynthetic rates, water use efficiencies and cumulative growth. In N. alessandrii, seedling survival was significantly greater on inoculated plants compared with non-inoculated individuals. For this species, a spatial distribution modeling revealed that, inoculation with root-fungal endophytes could potentially increase the current distributional range by almost threefold. Inoculation with root-fungal endophytes, did not reduce native rhizospheric microbiome diversity. Our results suggest that the studied consortium of Antarctic root-fungal endophytes improve the ecophysiological performance as well as the survival of inoculated trees and can be used as a biotechnological tool for the restoration of endangered tree species.

Forest canopy, a proxi of light intensity, arrests Pinus radiata invasion: basic science to conserve the Coastal Maulino forest, Central Chile

ABSTRACTCoastal Maulino forest is an endemic forest of central Chile, which has suffered a large history of disturbance, being replaced by large extensions of Pinus radiata plantations. This land transformation conveys high rates of pines invasion into native remnants. In this study we examined to what extent structural features of forest patches explains invisibility of this forest-type. Within eight forest fragments, we sampled 162 plots (10 x 10 m2 each). We quantified seedling pine density and related this estimates with tree cover, litter depth, PAR radiation, and diversity of the resident community. Our results indicate that canopy cover was the most important variable to determine seedling pine density within forest fragments. To preserve the Coastal Maulino forest and the biodiversity containing on it, it seems to be necessary to maintain the native canopy cover. These actions can be highly effective even if we cannot avoid a massive seed arrival from pine plantations which will be unable to regenerate under well conserved native forests.

Histiotus magellanicus (Chiroptera: Vespertilionidae) is not restricted to Subantarctic forests: first record for the Coastal Maulino Forest in central Chile

The southern Big-Eared Brown Bat, Histiotus magellanicus, is a poorly known vespertilionid occurring only in southern Chile and Argentina, where it appears to be restricted to Valdivian Temperate Forests and Magellanic Subpolar Forests. Here we report the first record of H. magellanicus in the Maulino Temperate Forest of central Chile, a Mediterranean-climate ecosystem, suggesting that the species might not be exclusive to subantarctic forests, as previously thought. This constitutes the northernmost continental record for the species, extending its range ca. 400 km the previous known northern locality