Improving Consistency of Photobiomodulation Therapy: A Novel Flat-Top Beam Hand-Piece versus Standard Gaussian Probes on Mitochondrial Activity

The tremendous therapeutic potential of photobiomodulation therapy in different branches of medicine has been described in the literature. One of the molecular mechanisms for this treatment implicates the mitochondrial enzyme, cytochrome C oxidase. However, the efficacy and consistency of clinical outcomes with photobiomodulation treatments has been fiercely debated. This work was motivated by this need to improve photobiomodulation devices and delivery approaches. We designed a novel hand-piece with a flat-top beam profile of irradiation. We compared the beam profile versus a standard hand-piece and a fibre probe. We utilized isolated mitochondria and performed treatments at various spots within the beam, namely, the centre, left and right edge. We examined mitochondrial activity by assessing ATP synthesis with the luciferin/luciferase chemiluminescent method as a primary endpoint, while mitochondrial damage was assessed as the secondary endpoint. We observed a uniform distribution of the power density with the flat-top prototype compared to a wide Gaussian beam profile with the standard fibre and standard hand-piece. We noted increased production of ATP in the centre of all three beams with respect to the non-treated controls (p < 0.05). Both the fibre and standard hand-piece demonstrated less increase in ATP synthesis at the edges than the centre (p < 0.05). In contrast, ATP synthesis was increased homogenously in the flat-top handpiece, both in the centre and the edges of the beam. Fibre, standard hand-piece and the flat-top hand-piece prototype have discrete beam distribution characteristics. This significantly affected the mitochondrial activity with respect to their position within the treated areas. Flat-top hand-piece enhances the uniformity of photobiomodulation treatments and can improve the rigour and reproducibility of PBM clinical outcomes.

Meta-heuristic optimization algorithms for synthesis of reconfigurable hexagonal array antenna in two principle vertical planes

This study illustrates the dynamical reconfiguration of a concentric hexagonal antenna array radiation to generate a pencil beam and flat-top beam simultaneously by electronic control in two principle vertical planes under consideration. Both the beams share a common normalized optimal current excitation amplitude distribution while the optimal sets of phase excitation coefficients are varied radically across the hexagons to generate a flat-top beam. The proposed approach is able to solve the underlying multi-objective problem and flexible enough to the efficient implementation of additional design constraints in the considered φ-planes. In this paper, a set of simulation-based examples are presented in an integrated way. The outcomes validate the effectiveness of the stated optimization using meta-heuristic optimization algorithms (teaching–learning-based optimization, symbiotic organism search, multi-verse optimization) to reach the solution globally and prove actual relevance to the concerned applications.

Multiple Solutions Starting from Real Shaped Beams in Equispaced Linear Arrays

In the present work, the theoretical basis of the multiplicity of solutions obtained from an initial real symmetric distribution is derived. This initial solution is devoted to generating an equivalent pure real shaped-beam pattern for a concrete synthesis scenario. However, these new solutions are not based on real symmetric distributions; hence, not based on the generation of pure real patterns. The bandwidth performances and tolerance to errors provided by the multiple solutions in the array design are analyzed by considering different architectures, also including mutual coupling models and element factor expressions due to accuracy purposes. In addition, a technique to obtain efficient linear arrays by designing resonant structures is addressed. Examples involving both standard linear arrays of half-wavelength cylindrical dipoles and resonant linear arrays generating flat-top beam patterns are reported and discussed. Additionally, an extension to planar arrays performed by means of a generalisation of the Baklanov transformation through collapsed distribution techniques inspired in the well-known method devised by Tseng and Cheng is performed. In such a way, an analysis of the quality of solutions for generating circular and elliptical footprints with controlled both SLL and ripple which are highly interesting in the framework of space vehicle applications.