An Anhydrous Sodium Chloride Skin Preservation Model for Studies on Keratinocytes Grafting into the Wounds

Background. Human skin is needed for covering large body areas lost by trauma. The shortcomings of contemporary methods of skin storage are limited preservation time and high immunogenicity if allogeneic. Methods. We investigated whether long-lasting skin preservation in anhydrous sodium chloride (NaCl) may be the source of keratinocytes (KCs) for transplantation. Dehydrated skin fragments were preserved for a time frame from 1 week to 12 months. Then, skin fragments were rehydrated, and KCs were isolated. The viability of KCs was assessed in viability/cytotoxicity test. NaCl-preserved KCs were cultured for 7 days and transplanted to the dorsum of SCID mice. Results. The morphology of NaCl-preserved KCs was unaltered. KCs from all epidermal layers could be identified. All grafts were accepted by the recipients. Transplanted KCs: synthesized keratins 10 and 16 expressed antigens specific for stem cells and transient-amplifying cells, and remained HLA-I-positive. Moreover, they expressed the proliferative marker PCNA. Cells isolated from transplants remained viable and produced enzymes. Conclusions. Transplantation of KCs obtained from human skin and stored in anhydrous NaCl may be considered for the closure of extensive skin wounds. The originality of this method consists of an effective storage procedure and easy preparation of keratinocytes for transplantation.

Pressure Data Analysis and Multilayer Modeling of a Gas-Condansate Reservoir

Kailastila gas field located at Golapgonj, Sylhet is one of the largest gas fields in Bangladesh. It produces a high amount of condensate along with natural gas. For the high values of GOR, it may be treated as a wet gas at reservoir condition. Three main sand reservoirs are confirmed in this field (upper, middle & lower).In this study, it has been shown that reservoir parameters of this gas field can be obtained for multilayered rectangular reservoir with formation cross-flow using pressure and their semi log derivative on a set of dimensionless type curve.The effects of the reservoir parameters such as permeability, skin, storage coefficient, and others such as reservoir areal extent and layering on the wellbore response, pressure are investigated.Shut in pressures are used in calculating permeability, skin factor, average reservoir pressure, wellbore storage effect and other reservoir properties. The direction of the formation cross flow is determined, first by the layer permeability and later by the skin factor.Finally, it is recommended to perform diagnostic analysis along with multilayer modeling to extract better results.Reservoir can also be considered as a multilayer cylindrical and can also use these models for other fields.