A convolution based computational approach towards DNA N6-methyladenine site identification and motif extraction in rice genome

DNA N6-methylation (6mA) in Adenine nucleotide is a post replication modification responsible for many biological functions. Automated and accurate computational methods can help to identify 6mA sites in long genomes saving significant time and money. Our study develops a convolutional neural network (CNN) based tool i6mA-CNN capable of identifying 6mA sites in the rice genome. Our model coordinates among multiple types of features such as PseAAC (Pseudo Amino Acid Composition) inspired customized feature vector, multiple one hot representations and dinucleotide physicochemical properties. It achieves auROC (area under Receiver Operating Characteristic curve) score of 0.98 with an overall accuracy of 93.97% using fivefold cross validation on benchmark dataset. Finally, we evaluate our model on three other plant genome 6mA site identification test datasets. Results suggest that our proposed tool is able to generalize its ability of 6mA site identification on plant genomes irrespective of plant species. An algorithm for potential motif extraction and a feature importance analysis procedure are two by products of this research. Web tool for this research can be found at: https://cutt.ly/dgp3QTR.

Systematic Analysis and Prediction ofIn SituCross Talk of O-GlcNAcylation and Phosphorylation

Reversible posttranslational modification (PTM) plays a very important role in biological process by changing properties of proteins. As many proteins are multiply modified by PTMs, cross talk of PTMs is becoming an intriguing topic and draws much attention. Currently, lots of evidences suggest that the PTMs work together to accomplish a specific biological function. However, both the general principles and underlying mechanism of PTM crosstalk are elusive. In this study, by using large-scale datasets we performed evolutionary conservation analysis, gene ontology enrichment, motif extraction of proteins with cross talk of O-GlcNAcylation and phosphorylation cooccurring on the same residue. We found that proteins within situO-GlcNAc/Phos cross talk were significantly enriched in some specific gene ontology terms and no obvious evolutionary pressure was observed. Moreover, 3 functional motifs associated with O-GlcNAc/Phos sites were extracted. We further used sequence features and GO features to predict O-GlcNAc/Phos cross talk sites based on phosphorylated sites and O-GlcNAcylated sites separately by the use of SVM model. The AUC of classifier based on phosphorylated sites is 0.896 and the other classifier based on GlcNAcylated sites is 0.843. Both classifiers achieved a relatively better performance compared with other existing methods.

Automatic Classification of Decorative Patterns in the Minoan Pottery of Kamares Style

An important feature of the Minoan culture is the pottery of Kamares style, that documents the Cretan cultural production between the first half of the 2nd millennium BC. This high level painted production, characterized by the combination of several diverse motifs, presents an enormous decorative repertoire. The extraordinary variety of combinations between elementary motifs according to a complex visual syntax makes interesting the automatic identification of the motifs, particularly upon potsherds. A complete pipeline to accomplish this task is still a challenge to Computer Vision and Pattern Recognition. Starting from a digital image ROI identification, motif extraction, robust contour detection should be performed to obtain a bag of digital shapes. In a second phase each of the extracted shapes has to be classified according to prototypes in a database produced by an expert. The co-occurrence of the different shapes in a specimen will, in turn, be used to help the archaeologists in the cultural and even chronological setting.