3-Pentylcatechol, a Non-Allergenic Urushiol Derivative, Displays Anti-Helicobacter pylori Activity In Vivo

We previously reported that 3-pentylcatechol (PC), a synthetic non-allergenic urushiol derivative, inhibited the growth of Helicobacter pylori in an in vitro assay using nutrient agar and broth. In this study, we aimed to investigate the in vivo antimicrobial activity of PC against H. pylori growing in the stomach mucous membrane. Four-week-old male C57BL/6 mice (n = 4) were orally inoculated with H. pylori Sydney Strain-1 (SS-1) for 8 weeks. Thereafter, the mice received PC (1, 5, and 15 mg/kg) and triple therapy (omeprazole, 0.7 mg/kg; metronidazole, 16.7 mg/kg; clarithromycin, 16.7 mg/kg, reference groups) once daily for 10 days. Infiltration of inflammatory cells in gastric tissue was greater in the H. pylori-infected group compared with the control group and lower in both the triple therapy- and PC-treated groups. In addition, upregulation of cytokine mRNA was reversed after infection, upon administration of triple therapy and PC. Interestingly, PC was more effective than triple therapy at all doses, even at 1/15th the dose of triple therapy. In addition, PC demonstrated synergism with triple therapy, even at low concentrations. The results suggest that PC may be more effective against H. pylori than established antibiotics.

Gastric mucosa structure in patients with different serum pepsinogen levels and ratios

Purpose of the study. Determine the gastric mucosa Structure in patients with different serum pepsinogen levels and ratios.Material and methods. 801 people (387 men, 414 women) underwent clinical examination and determination of pepsinogen-1, pepsinogen-2 and serum anti-Helicobacter pylori antibodies using GastroPanel (Biokhit, Finland). 161 patients with different levels of atrophy determined by serological screening method underwent a fibroesophagogastroduodenoscopy (Olimpus-10) with biopsy and subsequent morphological examination of the gastric mucosa performed using a visual analogue scale according to the Sydney Staging System. 107 patients had morphometry of the gastric mucosa with the determination of the number of central, parietal and mucoid cells.Results. The prevalence of severe atrophic gastritis in the stomach accounted for 10.9%. H. pylori was detected in 90.0% of the subjects. The morphological study showed atrophy in 94.4–95.8% of subjects with atrophic gastritis of the corpus mucosa of the stomach as defined by serological testing.Conclusions. Atrophy of the stomach mucous membrane as determined by morphological examination was prevalent in patients with severe atrophic gastritis of the corpus mucosa of the stomach, diagnosed using the method for determining serum pepsinogen levels.

Study of Wheals on Gastric Mucosa in Patients with Chronic Uticaria by Scanning and Transmission Electron Microscopy

Urticarial wheal is due to the leak of proteins and fluids to extravascula by increased capillary permeability. Edematous erythema and wheals located in the surface of gastric mucosa were observed by gastroscopy. The present contribution is the first in the international extent of the observation of wheals on gastric mucosa of 38 patients with chronic urticaria with the transmission electron microscope (TEM) and scanning electron microscope (SEM).Gastroscopies were performed on 38 patients with chronic urticaria during episodes ofcutaneous wheals. Biopsy specimens were taken from gastrical wheals from 38 patients. The EM specimens were routinely prepared. Linder the SEM, the surface of the epithelial cells of wheals on the gastric mucosa appeared slightly edematous and rough. Intercellular space of the epithelium became more narrow and shallow. The structure of microvillae on gastric mucosa epithelial cells could be seen, but appeared short and decreasing in amount. Under the TEM, it was found that the stomach mucous membrane was edematous.

A rare case of toxic gastritis with a discharge of the stomach mucous membrane

Abstracts. Internal diseases.F.L.Samsonov. A rare case of toxic gastritis with a discharge of the stomach mucous membrane. Doctor. 1901, 36.Patient M.V., 39 years old, was admitted to the hospital "Savich" with typical attacks of toxic gastritis after about 2 tablespoons of crude hydrochloric acid.