Hepatitis delta virus-like circular RNAs from diverse metazoans encode conserved hammerhead ribozymes

Human hepatitis delta virus (HDV) is a unique infectious agent whose genome is composed of a small circular RNA. Recent data, however, have reported the existence of highly divergent HDV-like circRNAs in the transcriptomes of diverse vertebrate and invertebrate species. The HDV-like RNAs described in amniotes such as birds and reptiles encode self-cleaving RNA motifs or ribozymes similar to the ones present in the human HDV, whereas no catalytic RNA domains have been reported for the HDV-like circRNAs detected in metagenomic data from some amphibians, fish, and invertebrates. Herein, we describe the self-cleaving motifs of the HDV-like genomes reported in newts and fish, which belong to the characteristic class of HDV ribozymes. Surprisingly, HDV-like circRNAs from a toad and a termite show conserved type-III hammerhead ribozymes, which belong to an unrelated class of catalytic RNAs characteristic of plant genomes and plant subviral circRNAs, such as some viral satellites and viroids. Sequence analyses revealed the presence of very similar HDV-like hammerhead ribozymes encoded in two termite genomes, but also in the genomes of several dipteran species. In vitro transcriptions confirmed the cleaving activity for these motifs, with moderate rates of self-cleavage. These data indicate that all described HDV-like agents contain self-cleaving motifs from either the HDV or the hammerhead class. Autocatalytic ribozymes in HDV-like genomes could be regarded as interchangeable domains and may have arisen from cellular transcriptomes, although we still cannot rule out some other evolutionary explanations.

Screening Carpet Substrate Interferences in Arson Identification by Solid Phase Microextraction and Gas Chromatography-Mass Spectrometry

The sample analysis and data interpretation is the most challenging step of fire debris analysis, due to the presence of combustion and pyrolysis products in the substrate material. In this study, a headspace solid phase microextraction (HS-SPME) procedure was applied to the extraction of combustion and pyrolysis products from three commonly used carpet substrate materials, made of nylon 6,6 and polyesters. Each carpet sample was burned with and without two different ignitable liquids (ILs), i.e., gasoline and kerosene, and the Total Ion Chromatograms (TICs) and Extracted Ion Profiles of characteristic class compounds of ILs were obtained and compared to those of unburned neat ILs, using gas-chromatography mass spectrometry (GC-MS), to study the possible interferences of these substrate materials in fire debris analysis.

Chemical Compounds and Biologic Activities: A Review of Cedrela Genus

The genus Cedrela P. Browne, which belongs to the Meliaceae family, has eighteen species. Trees of this genus are of economic interest due to wood quality, as well as being the focus of studies because of relevant biologic activities as in other Meliaceae species. These activities are mainly related to limonoids, a characteristic class of compounds in this family. Therefore, the aim of this review is to perform a survey of the citations in the literature on the Cedrela genus species. Articles were found on quantitative and qualitative phytochemical studies of the Cedrela species, revealing the chemical compounds identified, such as aliphatics acid and alcohol, flavonoids, tocopherol, monoterpenes, sesquiterpenes, triterpenes, cycloartanes, steroids, and limonoids. Although some activities were tested, the majority of studies focused on the insecticidal, antifeedant, or insect growth inhibitor activities of this genus. Nonetheless, the most promising activities were related to their antimalarial and antitripanocidal effects, although further investigations are still needed.

User-Centered Design for an Online Learning

Online learning has been studied for a long time. It has many benefits and challenges. In the period of COVID-19 spreading, many universities and schools have more concerns for their students of the virus infection. Thus, the online classroom has been set to be a teaching method for students. However, it is quite a new normal practice, especially in Thailand, to have an online instead of a face-to-face classroom. Therefore, this study is a path for preparing to conduct online learning. This study aims to provide guidelines to design effective online learning based on students' opinions. By analyzing the open-end questionnaires with 37 participants, the results reveal that blended learning is the most preferred learning pedagogical approach. Moreover, the metaphor factors, including subjects' characteristic, class period, class size, activities and assessment that were suitable for online learning according to the students' opinion, were suggested. Finally, some obstacles that students have faced in online learning were presented, and some solutions were proposed.