Fluorescein Binding to Normal Human Serum Proteins Demonstrated by Equilibrium Dialysis

1982 ◽  
Vol 100 (3) ◽  
pp. 484-487 ◽  
Author(s):  
W. Li ◽  
J. H. Rockey
Keyword(s):  
Human Serum ◽  
Serum Proteins ◽  
Equilibrium Dialysis ◽  
Normal Human Serum ◽  
Normal Human ◽  
Human Serum Proteins

scholarly journals The Impact of Graphite Oxide Nanocomposites on the Antibacterial Activity of Serum

2021 ◽  
Vol 22 (14) ◽  
pp. 7386
Author(s):  
Katarzyna Dorota Morka ◽  
Maciej Wernecki ◽  
Anna Kędziora ◽  
Marta Książczyk ◽  
Bartłomiej Dudek ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Human Serum ◽  
Complement System ◽  
Graphite Oxide ◽  
Galleria Mellonella ◽  
Serum Proteins ◽  
Normal Human Serum ◽  
Normal Human ◽  
The Impact ◽  
The Complement System

Nanoparticles can interact with the complement system and modulate the inflammatory response. The effect of these interactions on the complement activity strongly depends on physicochemical properties of nanoparticles. The interactions of silver nanoparticles with serum proteins (particularly with the complement system components) have the potential to significantly affect the antibacterial activity of serum, with serious implications for human health. The aim of the study was to assess the influence of graphite oxide (GO) nanocomposites (GO, GO-PcZr(Lys)2-Ag, GO-Ag, GO-PcZr(Lys)2) on the antibacterial activity of normal human serum (NHS), serum activity against bacteria isolated from alveoli treated with nanocomposites, and nanocomposite sensitivity of bacteria exposed to serum in vitro (using normal human serum). Additionally, the in vivo cytotoxic effect of the GO compounds was determined with application of a Galleria mellonella larvae model. GO-PcZr(Lys)2, without IR irradiation enhance the antimicrobial efficacy of the human serum. IR irradiation enhances bactericidal activity of serum in the case of the GO-PcZr(Lys)2-Ag sample. Bacteria exposed to nanocomposites become more sensitive to the action of serum. Bacteria exposed to serum become more sensitive to the GO-Ag sample. None of the tested GO nanocomposites displayed a cytotoxicity towards larvae.

Remazol Brilliant Blue as a pre-stain for the immediate visualization of human serum proteins on polyacrylamide gel disc electrophoresis.

1983 ◽  
Vol 29 (1) ◽  
pp. 42-44 ◽  
Author(s):  
A M Saoji ◽  
C Y Jad ◽  
S S Kelkar
Keyword(s):  
Human Serum ◽  
Room Temperature ◽  
Serum Proteins ◽  
Disc Electrophoresis ◽  
Brilliant Blue ◽  
Electrophoretic Mobilities ◽  
Amido Black ◽  
Normal Human ◽  
Human Serum Proteins

Abstract We describe the pre-staining of proteins in normal human serum with Remazol Brilliant Blue before separation by disc electrophoresis. Critical to reproducibility are: dye concentration of 0.16 mol/L in a Tris-glycine buffer (pH 8.3), use of equal volumes of serum and dye solution, a tagging period of 2 h at room temperature, and electrophoresis of 0.1 mL of the mixture at 2.5 mA/gel for about 2 h. Advantages include speed, quality of resolution, and low background. This method was compared with Amido Black post-electrophoresis staining in 35 sera. Of these, 16 showed identical results with respect to the number of bands. In the other 19, Amido Black showed more bands in the post-transferrin region and, sometimes, in the post-albumin region. The pre-stained gels showed slower electrophoretic mobilities of the components. Protein bands eluted from pre-stained gels retained immunological reactivity.

TESTOSTERONE BINDING BY HUMAN SERUM PROTEINS

1971 ◽  
Vol 68 (1_Supplb) ◽  
pp. S105 ◽  
Author(s):  
O. A. Lea ◽  
K. F. Støa
Keyword(s):  
Human Serum ◽  
Serum Proteins ◽  
Human Serum Proteins
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