Cytologic manifestations of cervical and vaginal infections. II. Confirmation of Chlamydia trachomatis infection by direct immunofluorescence using monoclonal antibodies

JAMA ◽  
1985 ◽  
Vol 253 (7) ◽  
pp. 997-1000 ◽  
Author(s):  
N. B. Kiviat
Keyword(s):  
Monoclonal Antibodies ◽  
Chlamydia Trachomatis ◽  
Direct Immunofluorescence ◽  
Vaginal Infections ◽  
Chlamydia Trachomatis Infection

scholarly journals Chlamydia trachomatis in women

2009 ◽  
Vol 62 (1-2) ◽  
pp. 7-12 ◽  
Author(s):  
Vera Jerant-Patic ◽  
Vesna Milosevic ◽  
Gordana Kozarev
Keyword(s):  
Monoclonal Antibodies ◽  
Chlamydia Trachomatis ◽  
Age Groups ◽  
Elisa Test ◽  
Direct Immunofluorescence ◽  
Igg Antibodies ◽  
Immunofluorescence Test ◽  
Vaginal Secretion ◽  
Chlamydia Trachomatis Infection ◽  
Clinical Diagnoses

The authors present results of a planned prospective study of infections with Chlamydia trachomatis in women. Direct immunofluorescence test (DIF) for proving Chlamydia trachomatis antigens in endocervical smear (by means of monoclonal antibodies), ELISA test for proving IgG antibodies against Chlamydia trachomatis in sera and RVK method for proving antibodies against group Chlamydia-antigen were applied to test 288 women (aged 19-67), previously thoroughly anamnestically and clinically examined. Chlamydia trachomatis infection was found in 29.51% of the cases, equally distributed in all age groups. The authors analyzed frequency of Chlamydia positive findings according to clinical diagnoses, parity, relevant data from gynecological history, as well as results of vaginal secretion tests and Papanicolaou smear findings.

Prevalence of Urogenital Chlamydia Trachomatis Infection in El Salvador. II. Gynaecology Outpatients

1992 ◽  
Vol 3 (6) ◽  
pp. 434-436 ◽  
Author(s):  
Ana Berta Cañas Posada ◽  
Jon Jonasson ◽  
Leonor de Linares ◽  
Solgun Bygdeman
Keyword(s):  
Chlamydia Trachomatis ◽  
El Salvador ◽  
Direct Immunofluorescence ◽  
Beta Lactamase ◽  
Gray Zone ◽  
Chlamydia Trachomatis Infection ◽  
San Salvador ◽  
Urogenital Infection ◽  
Healthy Women ◽  
Chlamydial Antigen

The prevalence of urogenital infection caused by Chlamydia trachomatis was examined in 100 non-pregnant women with cervicitis, and 100 healthy women, in San Salvador City, El Salvador. Pharmacia Chlamydia EIA test was used for the detection of chlamydial antigen in urethral and cervical specimens from all the women. Direct immunofluorescence was used for confirmative tests on the EIA positive and the negative gray zone samples. C. trachomatis antigen was detected in 28% of the women with cervicitis compared with 5% in the group of healthy women ( P < 0.001). The cervicitis group were also screened for Neisseria gonorrhoeae which was isolated from 12% of them. One strain out of 12 was beta-lactamase producing (PPNG). Five per cent of the women with cervicitis had simultaneous C. trachomatis and N. gonorrhoeae infections.

Localization of Chlamydia trachomatis infection by direct immunofluorescence and culture in pelvic inflammatory disease

1986 ◽  
Vol 154 (4) ◽  
pp. 865-873 ◽  
Author(s):  
Nancy B. Kiviat ◽  
Pål Wølner-Hanssen ◽  
Marcia Peterson ◽  
Judy Wasserheit ◽  
Walter E. Stamm ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Pelvic Inflammatory Disease ◽  
Inflammatory Disease ◽  
Direct Immunofluorescence ◽  
Chlamydia Trachomatis Infection

scholarly journals An application of fluorescein-conjugated monoclonal antibodies to the diagnosis of Chlamydia trachomatis infection in children.

1985 ◽  
Vol 145 (1) ◽  
pp. 57-63 ◽  
Author(s):  
KEI NUMAZAKI ◽  
SHUNZO CHIBA ◽  
TERUAKI MOROBOSHI ◽  
NOBUTADA TACHI ◽  
KOKI AOKI ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Chlamydia Trachomatis ◽  
Chlamydia Trachomatis Infection

scholarly journals The detection ofChlamydia trachomatisby direct immunofluorescence in conjunctival smears from patients with trachoma and patients with ophthalmia neonatorum using a conjugated monoclonal antibody

1986 ◽  
Vol 96 (1) ◽  
pp. 83-87 ◽  
Author(s):  
D. C. W. Mabey ◽  
S. Booth-Mason
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Chlamydia Trachomatis ◽  
Sensitivity And Specificity ◽  
Direct Immunofluorescence ◽  
Conjunctival Epithelium ◽  
Cotton Wool ◽  
Minimum Requirement ◽  
Ophthalmia Neonatorum ◽  
Immunofluorescent Technique

SUMMARYDuplicate specimens were taken with cotton-wool swabs from the upper tarsal conjunctiva of 63 patients living in Gambian villages in which trachoma is endemic and from 34 infants with ophthalmia neonatorum (ON) attending an outpatient clinic in The Gambia. The detection ofChlamydia trachomatisby direct immunofluorescence (IF) using a conjugated monoclonal antibody to its principal outer membrane protein was compared with isolation in cycloheximide-treated McCoy cells. For trachoma, the sensitivity and specificity of the immunofluorescent technique were 62% and 100% respectively if ten elementary bodies (EBs) was taken as the minimum requirement for positivity by IF. If all cases with one or more EB were considered positive, the sensitivity was 81% and the specificity 85%. For ON the sensitivity and specificity were 100% and 95% respectively, regardless of which criterion was used. In view of its simplicity and easy applicability to field conditions it seems likely that direct IF using monoclonal antibodies may be a useful technique for the detection ofC. trachomatisin the conjunctival epithelium of patients with trachoma.

scholarly journals The diagnosis of Chlamydia trachomatis cervical infection among students by using classical and molecular methods

2013 ◽  
Vol 141 (3-4) ◽  
pp. 187-191
Author(s):  
Snezana Tomanovic ◽  
Ivan Cukic ◽  
Marija Obradovic ◽  
Nadja Curcic ◽  
Andjela Petrovic-Milinkovic ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Sensitivity And Specificity ◽  
Uterine Cervix ◽  
Transmitted Disease ◽  
Molecular Methods ◽  
Nucleic Acid Hybridization ◽  
Direct Immunofluorescence ◽  
Chlamydia Trachomatis Infection ◽  
Molecular Tests ◽  
Outpatient Unit

Introduction. Chlamydia trachomatis is the most common cause of sexually transmitted disease. The possibility of serious complications may be prevented by early detection of the bacteria on the uterine cervix by the application of sensitive and reliable tests such as up-to-date molecular tests. Objective. The aim of the study was the comparison of sensitivity and specificity of three different methods in the diagnosis of Chlamydia trachomatis infection. Methods. The study included 69 female students referred to the gynecological outpatient unit at the Students? Polyclinic for colposcopic examination of the uterine cervix. Cervical Chlamydia trachomatis was diagnosed by using three different methods: direct immunofluorescence (DIF), nucleic acid hybridization assay (hc2), and polymerase chain reaction (PCR). Results. By using DIF Chlamydia trachomatis was identified in four students (5.80%), by using hc2 also in four (5.80%), while by using PCR test in six students (8.70%). Comparative analysis of the obtained results evidenced sensitivity and specificity rates of DIF in comparison to PCR method of 46% and 95%, respectively. Sensitivity and specificity of DIF method in comparison to hc2 was 62% and 97%. Sensitivity and specificity of hc2 method in comparison to PCR was 76% and 100%. Conclusion. Contemporary molecular methods, such as PCR, are methods of choice for the identification of endocervical Chlamydia trachomatis in the population of university students without symptoms of the disease.

Prevalence of Urogenital Chlamydia Trachomatis Infection in El Salvador I. Infection during Pregnancy and Perinatal Transmission

1992 ◽  
Vol 3 (1) ◽  
pp. 33-37 ◽  
Author(s):  
Ana Berta Cañas Posada ◽  
Jon Jonasson ◽  
Leonor de Linares ◽  
Solgun Bygdeman
Keyword(s):  
Chlamydia Trachomatis ◽  
Pregnant Women ◽  
El Salvador ◽  
Chlamydial Infection ◽  
Perinatal Transmission ◽  
Direct Immunofluorescence ◽  
Chlamydia Trachomatis Infection ◽  
San Salvador ◽  
Age Range ◽  
Chlamydial Antigen

One-hundred and twenty-nine pregnant women in labour (age range 15–46 years; median age 23) and 42 infants born to chlamydia-positive mothers (age range 5–15 days; median age 10) were investigated to estimate the prevalence and incidence, respectively, of Chlamydia trachomatis infection in San Salvador, El Salvador. Urethral and cervical samples were obtained from all women and conjunctival specimens were taken from both eyes of each child. The chlamydial antigen was detected with the commercial Pharmacia Chlamydia EIA kit. Direct immunofluorescence (DFA) (Syva MicroTrak) was used for confirmation. In the newborns both EIA and DFA tests on direct preparations from ocular smears were performed on all the samples. The prevalence of chlamydial infection in pregnant women was 44% (57/129). The incidence of chlamydial infection in neonates was 64% (27/42), and the majority of the infected children (56%) had conjunctivitis. Referring to individuals rather than specimens the sensitivity of EIA tests on conjunctival samples from the infants was low (37%) as compared with 91% on urethral and cervical specimens from the pregnant women.

scholarly journals Classical and molecular methods for diagnosis of Chlamydia trachomatis infections

2011 ◽  
Vol 64 (9-10) ◽  
pp. 477-480 ◽  
Author(s):  
Snezana Tomanovic ◽  
Slobodanka Djukic
Keyword(s):  
Nucleic Acid ◽  
Chlamydia Trachomatis ◽  
Deoxyribonucleic Acid ◽  
Nucleic Acid Amplification ◽  
Clinical Samples ◽  
Direct Immunofluorescence ◽  
Nucleic Acid Amplification Tests ◽  
Chlamydia Trachomatis Infection ◽  
Culture Cells ◽  
Chlamydial Infections

Introduction. Genital Chlamydia trachomatis infection is the leading cause of bacterial sexually transmitted diseases in industrial countries, particularly among young people. The consequences of chlamydial infections may involve pelvic inflammatory disease, ectopic pregnancy and tubal factor infertility. Methods. Available tests for detection of chlamydia in men and women include culture in tissue culture cells, direct immunofluorescence test, enzyme immune assay, nucelic acid probe hibridization and polymerase chain reaction. Nucleic acid amplification tests use different ribonucleic and deoxyribonucleic acid regions as target molecules for amplifying Chlamydia trachomatis ribonucleic/deoxyribonucleic acid in clinical samples. Nucleic acid amplification tests are more sensitive than non-nucleic acid amplification tests. Conclusion. Although screening programmes exist in a number of countries, the continuously increasing prevalence of chlamydial infections demonstrates the necessity for defining the best method for the diagnosis and the population for screening.

Sign in / Sign up

Export Citation Format

Share Document