2021 ◽  
Vol 9 (2) ◽  
pp. 243
Author(s):  
Akihiko Suzuki ◽  
Miwa Suzuki

In the present study, we isolated and characterized Lactococcus lactis (L. lactis) subsp. lactis from a female Cuvier’s beaked whale (Ziphius cavirostris) stranded in Shizuoka, Japan. Only five isolates (CBW1-5), grown on Lactobacilli de Man Rogosa Sharpe (MRS) agar plates prepared using 50% artificial seawater, were positive in L. lactis species-specific primer PCR. Their 16S rRNA sequences were highly similar to those of L. lactis subsp. lactis JCM 5805T. The Gram reaction, motility, gas production from glucose, catalase production, and growth conditions were consistent with those of the type strain. Additionally, carbohydrate utilization of the strains was consistent with previously reported marine organism-derived strains. The pH-neutralized cell-free culture supernatant of strain CBW2 inhibited the growth of Bacillus subtilis subsp. subtilis ATCC 6051 and Vibrio alginolyticus ATCC 17749, whereas protease treatment eliminated or diminished its inhibitory activity. The strain possesses a precursor of the nisin structural gene (nisA), which showed 100% homology with nisin Z, and nisin biosynthesis-related genes (nisB, nisC, nisT, nisP, nisF, nisI, and nisRK), suggesting that the strain produces a nisin-like substance. This study provides fundamental information on whale-derived L. lactis subsp. lactis which may be useful for reducing the carriage of B. subtilis subsp. subtilis and V. alginolyticus.


2003 ◽  
Vol 46 (5) ◽  
pp. 385-388 ◽  
Author(s):  
Sang-Hee Park ◽  
Kikuji Itoh ◽  
Eisaku Kikuchi ◽  
Hidekazu Niwa ◽  
Tomohiko Fujisawa

2004 ◽  
Vol 67 (5) ◽  
pp. 928-933 ◽  
Author(s):  
NATALIA RILLA ◽  
BEATRIZ MARTÍNEZ ◽  
ANA RODRÍGUEZ

Methicillin-resistant Staphylococcus aureus strains are a potential threat for food safety because foodborne illness caused by methicillin-resistant Staphylococcus aureus has been reported even though these strains were only associated with nosocomial infections until recently. This article focuses on the inhibitory effect of the nisin Z–producing strain Lactococcus lactis subsp. lactis IPLA 729 on the growth of Staphylococcus aureus CECT 4013, a methicillin-resistant strain. S. aureus was inhibited by the presence of the nisin producer IPLA 729 in buffered Trypticase soy broth, milk, and Afuega'l Pitu cheese, an acid-coagulated cheese manufactured in Asturias, Northern Spain. A reduction of 3.66 log units was observed in Trypticase soy broth at the end of the incubation period. In milk, viable counts of S. aureus were undetectable or were reduced by 2.16 log units in 24 h depending on the initial inoculum (1.8 × 104 and 7.2 × 106 CFU/ml). The staphylococcal strain was also undetected in test cheeses in which the nisin Z producer was present whereas 2 log units were detected in control cheeses at the end of ripening.


1999 ◽  
Vol 53 (2-3) ◽  
pp. 141-152 ◽  
Author(s):  
N.A Olasupo ◽  
U Schillinger ◽  
A Narbad ◽  
H Dodd ◽  
W.H Holzapfel
Keyword(s):  

2011 ◽  
Vol 127 (2) ◽  
pp. 847-854 ◽  
Author(s):  
N. Schneider ◽  
K. Werkmeister ◽  
M. Pischetsrieder
Keyword(s):  

2005 ◽  
Vol 7 (14) ◽  
pp. 2961-2964 ◽  
Author(s):  
Nourdin Ghalit ◽  
Alex J. Poot ◽  
Alois Fürstner ◽  
Dirk T. S. Rijkers ◽  
Rob M. J. Liskamp

1996 ◽  
Vol 235 (1-2) ◽  
pp. 267-274 ◽  
Author(s):  
Rudolf A. Demel ◽  
Tamara Peelen ◽  
Roland J. Siezen ◽  
Ben Kruijff ◽  
Oscar P. Kuipers

2018 ◽  
Vol 8 ◽  
Author(s):  
Taskeen Niaz ◽  
Saima Shabbir ◽  
Tayyaba Noor ◽  
Rashda Abbasi ◽  
Zulfiqar A. Raza ◽  
...  

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