In Vitro Enrichment of Tumor‐Initiating Cells from Human Established Cell Lines

Short-term experiments were used to investigate the effects of various concentrations of methanol and formaldehyde upon cells grown in vitro. Mouse liver epithelial, HeLa, human lung, and skin cells were exposed to several concentrations of methanol. The same cell lines, except HeLa, were subjected to different concentrations of formaldehyde.Relatively high concentrations of methanol were required to produce rapid toxic effects. Not all cell lines responded alike to methanol. Concentrations of 15 mg/ml were decidedly inhibitory in case of human skin, lung, and HeLa cells. This concentration, however, appeared to enhance growth in liver cells. At higher concentrations the methanol was toxic to liver cells as well as to the others.Concentrations of formaldehyde greater than 0.035 mg/ml were toxic to mouse liver, human lung and skin cells. A concentration of 0.01 mg/ml of formaldehyde inhibited proliferation of these same cells. These three cell lines did not appear to differ significantly in respect to their sensitivity to formaldehyde. Of the substances investigated to date, formaldehyde appears to be the most toxic.

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Effects of Physicochemical Properties of Polyacrylamide (PAA) and Polydimethylsiloxane (PDMS) on Cardiac Cell behavior

Soft Matter ◽

10.1039/d0sm01986k ◽

2021 ◽

Author(s):

Karim Daliri ◽

Kurt Pfannkuche ◽

Bora Garipcan

Keyword(s):

Cell Lines ◽

Cultured Cells ◽

Cell Behavior ◽

Cardiac Cell ◽

Established Cell Lines ◽

The World ◽

Established Cell ◽

Primary Origin ◽

Transformed Cell Lines

In vitro cell culture is commonly applied in laboratories around the world. Cultured cells are either of primary origin or established cell lines. Such transformed cell lines are increasingly replaced...

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Cell lines with extended in vitro growth potential from human renal proximal tubule: Characterization, response to inducers, and comparison with established cell lines

Journal of Laboratory and Clinical Medicine ◽

10.1016/s0022-2143(97)90180-3 ◽

1997 ◽

Vol 129 (3) ◽

pp. 318-329 ◽

Cited By ~ 174

Author(s):

Lorraine C. Racusen ◽

C. Monteil ◽

Anita Sgrignoli ◽

Margit Lucskay ◽

S. Marouillat ◽

...

Keyword(s):

Proximal Tubule ◽

Cell Lines ◽

Renal Proximal Tubule ◽

Growth Potential ◽

In Vitro Growth ◽

Established Cell Lines ◽

Established Cell

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THE EFFECTS OF SULPHUR DIOXIDE UPON ESTABLISHED CELL LINES CULTIVATED IN VITRO

Canadian Journal of Biochemistry and Physiology ◽

10.1139/o62-025 ◽

1962 ◽

Vol 40 (2) ◽

pp. 207-217 ◽

Cited By ~ 22

Author(s):

James R. Thompson ◽

Donald M. Pace

Keyword(s):

Cell Lines ◽

Hela Cells ◽

Sulphur Dioxide ◽

Mouse Liver ◽

Recovery Period ◽

Inhibition Of Growth ◽

Established Cell Lines ◽

Established Cell ◽

Stimulation Of

Studies have been made on the effects of SO2 and its salts on strain L, mouse liver, and HeLa cells. Of the cell lines tested, the HeLa cells seemed to be more sensitive to SO2 and its salts than the cells of mouse origin.Cells cultivated in "biological" medium grow in concentrations of gaseous SO2 up to 2000 p.p.m., although somewhat inhibited. Cells subjected to a concentration of 500 p.p.m. in this medium are not affected greatly and their growth is comparable to those cells in control cultures.The addition of various salts of SO2 (Na2SO4, Na2SO3, and NaHSO3) in concentrations from 10 to 200 mg% produced responses ranging from complete inhibition of growth (by 200 mg% NaHSO3) to apparent stimulation of growth by some concentrations of Na2SO4. Toxicity of these salts was in the order of NaHSO3 > Na2SO3 > Na2SO4.When cells in vitro are directly exposed to SO2 in specially designed culture flasks, strain L cells are apparently able to tolerate 5 p.p.m. SO2 for five 8-hour exposure intervals, provided a "recovery" period follows each exposure.Certain components of serum seem to play a very important role as protective agents in modifying the effect of gaseous SO2, possibly by combination.

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Vascular phase of Sarcocystis cruzi cultured in vitro

Canadian Journal of Zoology ◽

10.1139/z86-033 ◽

1986 ◽

Vol 64 (1) ◽

pp. 209-211 ◽

Cited By ~ 11

Author(s):

C. A. Speer ◽

J. P. Dubey

Keyword(s):

Endothelial Cells ◽

Pulmonary Artery ◽

Cell Lines ◽

Sarcocystis Spp ◽

Established Cell Lines ◽

Established Cell ◽

Sarcocystis Cruzi

Sporozoites of Sarcocystis cruzi penetrated various established cell lines but developed to meronts in bovine monocytes (BM) and bovine pulmonary artery endothelial cells (CPA) only. At 31 to 50 days after sporozoite inoculation, approximately four times more merozoites were harvested from cultures of CPA (72.7 × 106 merozoites) than from BM (16.6 × 106 merozoites). The ability to grow merogonous stages in vitro can now be used to elucidate pathogenetic and immunologic mechanisms associated with infection by Sarcocystis spp.

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