scholarly journals Hypoxia affects in vitro growth of newly established cell lines from patients with malignant pleural mesothelioma

2013 ◽  
Vol 34 (1) ◽  
pp. 13-21 ◽  
Author(s):  
Houman Goudarzi ◽  
Yasuhiro Hida ◽  
Hiroko Takano ◽  
Hiroki Teramae ◽  
Hisashi Iizasa ◽  
...  
Keyword(s):  
Cell Lines ◽  
Malignant Pleural Mesothelioma ◽  
Pleural Mesothelioma ◽  
In Vitro Growth ◽  
Established Cell Lines ◽  
Established Cell

Sensitivity to Amphotericin B of in vitro Established Cell Lines

Nature ◽  
1974 ◽  
Vol 247 (5441) ◽  
pp. 466-469 ◽  
Author(s):  
P. AMATI ◽  
C. LAGO
Keyword(s):  
Amphotericin B ◽  
Cell Lines ◽  
Established Cell Lines ◽  
Established Cell

scholarly journals Antagonists of growth hormone-releasing hormone (GHRH) inhibit the growth of human malignant pleural mesothelioma

2019 ◽  
Vol 116 (6) ◽  
pp. 2226-2231 ◽  
Author(s):  
Tania Villanova ◽  
Iacopo Gesmundo ◽  
Valentina Audrito ◽  
Nicoletta Vitale ◽  
Francesca Silvagno ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Growth Factor ◽  
Cell Lines ◽  
Malignant Pleural Mesothelioma ◽  
Pleural Mesothelioma ◽  
Growth Hormone Releasing Hormone ◽  
Releasing Hormone ◽  
Ghrh Antagonists

Malignant pleural mesothelioma (MPM) is an aggressive malignancy associated with exposure to asbestos, with poor prognosis and no effective therapies. The strong inhibitory activities of growth hormone-releasing hormone (GHRH) antagonists have been demonstrated in different experimental human cancers, including lung cancer; however, their role in MPM remains unknown. We assessed the effects of the GHRH antagonists MIA-602 and MIA-690 in vitro in MPM cell lines and in primary MPM cells, and in vivo in MPM xenografts. GHRH, GHRH receptor, and its main splice variant SV1 were found in all the MPM cell types examined. In vitro, MIA-602 and MIA-690 reduced survival and proliferation in both MPM cell lines and primary cells and showed synergistic inhibitory activity with the chemotherapy drug pemetrexed. In MPM cells, GHRH antagonists also regulated activity and expression of apoptotic molecules, inhibited cell migration, and reduced the expression of matrix metalloproteinases. These effects were accompanied by impairment of mitochondrial activity and increased production of reactive oxygen species. In vivo, s.c. administration of MIA-602 and MIA-690 at the dose of 5 μg/d for 4 wk strongly inhibited the growth of MPM xenografts in mice, along with reduction of tumor insulin-like growth factor-I and vascular endothelial growth factor. Overall, these results suggest that treatment with GHRH antagonists, alone or in association with chemotherapy, may offer an approach for the treatment of MPM.

scholarly journals Evaluation of essential oils from 22 Guatemalan medicinal plants for in vitro activity against cancer and established cell lines

2018 ◽  
Vol 12 (3) ◽  
pp. 42-49 ◽  
Author(s):  
Byron Miller Andrew ◽  
Gordon Cates Rex ◽  
O’Neill Kim ◽  
Alfonso Fuentes Soria Juan ◽  
Vicente Espinoza Luis ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Essential Oils ◽  
Cell Lines ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
Established Cell Lines ◽  
Established Cell

OBSERVATIONS ON THE EFFECTS OF METHANOL AND FORMALDEHYDE ON ESTABLISHED CELL LINES CULTIVATED IN VITRO

1963 ◽  
Vol 41 (2) ◽  
pp. 299-304 ◽  
Author(s):  
Alice Elliott ◽  
Donald M. Pace
Keyword(s):  
Cell Lines ◽  
Mouse Liver ◽  
Human Lung ◽  
Liver Cells ◽  
Short Term ◽  
Skin Cells ◽  
Established Cell Lines ◽  
Established Cell ◽  
High Concentrations

Short-term experiments were used to investigate the effects of various concentrations of methanol and formaldehyde upon cells grown in vitro. Mouse liver epithelial, HeLa, human lung, and skin cells were exposed to several concentrations of methanol. The same cell lines, except HeLa, were subjected to different concentrations of formaldehyde.Relatively high concentrations of methanol were required to produce rapid toxic effects. Not all cell lines responded alike to methanol. Concentrations of 15 mg/ml were decidedly inhibitory in case of human skin, lung, and HeLa cells. This concentration, however, appeared to enhance growth in liver cells. At higher concentrations the methanol was toxic to liver cells as well as to the others.Concentrations of formaldehyde greater than 0.035 mg/ml were toxic to mouse liver, human lung and skin cells. A concentration of 0.01 mg/ml of formaldehyde inhibited proliferation of these same cells. These three cell lines did not appear to differ significantly in respect to their sensitivity to formaldehyde. Of the substances investigated to date, formaldehyde appears to be the most toxic.

scholarly journals Effects of Physicochemical Properties of Polyacrylamide (PAA) and Polydimethylsiloxane (PDMS) on Cardiac Cell behavior

Soft Matter ◽  
2021 ◽  
Author(s):  
Karim Daliri ◽  
Kurt Pfannkuche ◽  
Bora Garipcan
Keyword(s):  
Cell Lines ◽  
Cultured Cells ◽  
Cell Behavior ◽  
Cardiac Cell ◽  
Established Cell Lines ◽  
The World ◽  
Established Cell ◽  
Primary Origin ◽  
Transformed Cell Lines

In vitro cell culture is commonly applied in laboratories around the world. Cultured cells are either of primary origin or established cell lines. Such transformed cell lines are increasingly replaced...

scholarly journals MALAT1 promotes malignant pleural mesothelioma by sponging miR-141-3p

Open Medicine ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. 1653-1667
Author(s):  
Pei Wang ◽  
Cuiwei Bai ◽  
Shasha Shen ◽  
Chang Jiang ◽  
Jie Deng ◽  
...  
Keyword(s):  
Cell Lines ◽  
Malignant Pleural Mesothelioma ◽  
Luciferase Reporter ◽  
Migration And Invasion ◽  
Pleural Mesothelioma ◽  
Hippo Signaling ◽  
Downstream Target ◽  
Competitive Endogenous Rna

Abstract The aim of this study was to clarify the role of lncRNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in proliferation, migration, and invasion of malignant pleural mesothelioma (MPM) cells. The quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used to detect the expression of MALAT1 in MPM cell lines. The effects of MALAT1 and miR-141-3p on the proliferation, migration, and invasion of MPM cells were studied through a series of in vitro cellular experiments. The flow cytometry was utilized to detect the cell apoptosis. The dual‐luciferase reporter assay was employed to explore the binding relationship among MALAT1, miR-141-3p, and YES-associated protein 1 (YAP1). MALAT1 was overexpressed in MPM cell lines, while its knockdown significantly inhibited the cell proliferation, migration, and invasion, and increased the number of MPM cells in the G0/G1 phase. In addition, MALAT1 could directly bind to miR-141-3p and inhibit its expression. YAP1 has been identified as a downstream target of miR-141-3p, and its expression level was inhibited by miR-141-3p. MALAT1 can be used as a competitive endogenous RNA (ceRNA) to regulate the YAP1-Hippo signaling pathway through miR-141-3p, promote the proliferation, migration, and invasion of MPM cells, and provide a new target for the therapy of MPM.

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