scholarly journals Effects of Physicochemical Properties of Polyacrylamide (PAA) and Polydimethylsiloxane (PDMS) on Cardiac Cell behavior

Soft Matter ◽  
2021 ◽  
Author(s):  
Karim Daliri ◽  
Kurt Pfannkuche ◽  
Bora Garipcan
Keyword(s):  
Cell Lines ◽  
Cultured Cells ◽  
Cell Behavior ◽  
Cardiac Cell ◽  
Established Cell Lines ◽  
The World ◽  
Established Cell ◽  
Primary Origin ◽  
Transformed Cell Lines

In vitro cell culture is commonly applied in laboratories around the world. Cultured cells are either of primary origin or established cell lines. Such transformed cell lines are increasingly replaced...

scholarly journals Murine Gammaherpesvirus (MHV-68) Transforms Cultured Cells in vitro

Intervirology ◽  
2015 ◽  
Vol 58 (2) ◽  
pp. 69-72 ◽  
Author(s):  
Veronika Mrázová ◽  
Tatiana Betáková ◽  
Marcela Kúdelová ◽  
Miroslava Šupolíková ◽  
Veronika Lachová ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cultured Cells ◽  
Virus Antigen ◽  
Immunofluorescence Assay ◽  
Dermal Fibroblasts ◽  
Murine Gammaherpesvirus ◽  
Transformed Cell ◽  
Transformed Cell Lines ◽  
Nih 3T3

Human dermal fibroblasts and mouse NIH/3T3 cells acquired the transformed phenotype (‘criss-cross' pattern of growth) after infection with ultraviolet-irradiated murine gammaherpesvirus (MuHV-4 strain 68; MHV-68). These cells with changed phenotype could be serially cultured for 5-6 passages (35-40 days), and then they entered into crisis and most of them died. In a small number of cultures, however, foci of newly transformed cells appeared from which two stable cell lines were derived. After 6-9 cell culture passages of the MHV-68 transformed cell lines, MHV-68 DNA and virus antigen could be detected by PCR and immunofluorescence assay along with the disappearance of actin bundles, indicating that both transformed cell lines might be oncogenic.

Sensitivity to Amphotericin B of in vitro Established Cell Lines

Nature ◽  
1974 ◽  
Vol 247 (5441) ◽  
pp. 466-469 ◽  
Author(s):  
P. AMATI ◽  
C. LAGO
Keyword(s):  
Amphotericin B ◽  
Cell Lines ◽  
Established Cell Lines ◽  
Established Cell

scholarly journals Studies of fibronectin matrices in living cells with fluoresceinated gelatin.

1980 ◽  
Vol 87 (1) ◽  
pp. 14-22 ◽  
Author(s):  
P Hsieh ◽  
R Segal ◽  
L B Chen
Keyword(s):  
Extracellular Matrix ◽  
Chick Embryo ◽  
Cell Surface ◽  
Cell Lines ◽  
Cultured Cells ◽  
Living Cells ◽  
Low Concentration ◽  
Established Cell Lines ◽  
The Matrix ◽  
Established Cell

We have used fluorescein isosthiocyanate-conjugated gelatin (FITC-gelatin) (1 mg/ml) to localize cell surface fibronectin in unfixed live cells in cultures. FITC-gelatin stains the fibronectin matrix on primary cultures of rat and chick embryo fibroblasts as well as untransformed, established cell lines. In live cultured cells, fibronectin in many areas of the extracellular matrix is inaccessible to antibody and cannot be visualized by immunofluorescence staining. In contrast, fibronectin in these areas is fully stainable by FITC-gelatin. At a low concentration (20 micrograms/ml), FITC-gelatin stains the fibronectin matrix of primary cultured cells but not of "untransformed" established cell lines. SEM can detect only the matrix stainable with the low concentration of FITC-gelatin, such as that expressed by primary chick embryo fibroblasts. The binding of fibronectin to the extracellular matrix is very stable and FITC-gelatin remained bound to the matrix for at least 10 d in culture. Radioiodinated gelatin has been used to quantitate the level of cell surface fibronectin in living normal and transformed cells. FITC-gelatin appears to be a useful probe for studying the fibronectin of living cells in culture.

scholarly journals Hypoxia affects in vitro growth of newly established cell lines from patients with malignant pleural mesothelioma

2013 ◽  
Vol 34 (1) ◽  
pp. 13-21 ◽  
Author(s):  
Houman Goudarzi ◽  
Yasuhiro Hida ◽  
Hiroko Takano ◽  
Hiroki Teramae ◽  
Hisashi Iizasa ◽  
...  
Keyword(s):  
Cell Lines ◽  
Malignant Pleural Mesothelioma ◽  
Pleural Mesothelioma ◽  
In Vitro Growth ◽  
Established Cell Lines ◽  
Established Cell

scholarly journals Production of plasminogen activator by established cell lines of mouse origin.

1977 ◽  
Vol 73 (1) ◽  
pp. 47-55 ◽  
Author(s):  
D B Rifkin ◽  
R Pollack
Keyword(s):  
Plasminogen Activator ◽  
Cell Lines ◽  
3T3 Cells ◽  
Established Cell Lines ◽  
Embryo Cultures ◽  
Established Cell ◽  
Swiss 3T3 ◽  
Swiss 3T3 Cell ◽  
Transformed Cell Lines ◽  
3T3 Cell Lines

The correlation between malignant transformation and increased plasminogen activator synthesis has been studied in a variety of established cell lines. In contrast to the behavior of secondary mouse embryo cultures, which always show increased fibrinolytic activity when transformed, no such correlation was found within the BALB/c 3T3 line and its transformed derivatives. Cell lines were established from tumors initiated in BALB/c mice by several transformed cell lines. These lines were generally found to contain no more plasminogen activator than the cells used for inoculation. A correlation was found between transformation and plasminogen activator synthesis within Swiss 3T3 cell lines. However, the correlation was not maintained by serum revertants of transformed Swiss 3T3 cells.

scholarly journals Evaluation of essential oils from 22 Guatemalan medicinal plants for in vitro activity against cancer and established cell lines

2018 ◽  
Vol 12 (3) ◽  
pp. 42-49 ◽  
Author(s):  
Byron Miller Andrew ◽  
Gordon Cates Rex ◽  
O’Neill Kim ◽  
Alfonso Fuentes Soria Juan ◽  
Vicente Espinoza Luis ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Essential Oils ◽  
Cell Lines ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
Established Cell Lines ◽  
Established Cell

OBSERVATIONS ON THE EFFECTS OF METHANOL AND FORMALDEHYDE ON ESTABLISHED CELL LINES CULTIVATED IN VITRO

1963 ◽  
Vol 41 (2) ◽  
pp. 299-304 ◽  
Author(s):  
Alice Elliott ◽  
Donald M. Pace
Keyword(s):  
Cell Lines ◽  
Mouse Liver ◽  
Human Lung ◽  
Liver Cells ◽  
Short Term ◽  
Skin Cells ◽  
Established Cell Lines ◽  
Established Cell ◽  
High Concentrations

Short-term experiments were used to investigate the effects of various concentrations of methanol and formaldehyde upon cells grown in vitro. Mouse liver epithelial, HeLa, human lung, and skin cells were exposed to several concentrations of methanol. The same cell lines, except HeLa, were subjected to different concentrations of formaldehyde.Relatively high concentrations of methanol were required to produce rapid toxic effects. Not all cell lines responded alike to methanol. Concentrations of 15 mg/ml were decidedly inhibitory in case of human skin, lung, and HeLa cells. This concentration, however, appeared to enhance growth in liver cells. At higher concentrations the methanol was toxic to liver cells as well as to the others.Concentrations of formaldehyde greater than 0.035 mg/ml were toxic to mouse liver, human lung and skin cells. A concentration of 0.01 mg/ml of formaldehyde inhibited proliferation of these same cells. These three cell lines did not appear to differ significantly in respect to their sensitivity to formaldehyde. Of the substances investigated to date, formaldehyde appears to be the most toxic.

Sign in / Sign up

Export Citation Format

Share Document