Set up and Characterization of Reference Measuring Systems for On-site Live Verification of HV Instrument Transformers

2010 ◽  
pp. 543-556 ◽  
Author(s):  
G. Crotti ◽  
A. Sardi ◽  
N. Kuljaca ◽  
P. Mazza ◽  
G. de Don
2019 ◽  
Author(s):  
Priya Prakash ◽  
Travis Lantz ◽  
Krupal P. Jethava ◽  
Gaurav Chopra

Amyloid plaques found in the brains of Alzheimer’s disease (AD) patients primarily consists of amyloid beta 1-42 (Ab42). Commercially, Ab42 is synthetized using peptide synthesizers. We describe a robust methodology for expression of recombinant human Ab(M1-42) in Rosetta(DE3)pLysS and BL21(DE3)pLysS competent E. coli with refined and rapid analytical purification techniques. The peptide is isolated and purified from the transformed cells using an optimized set-up for reverse-phase HPLC protocol, using commonly available C18 columns, yielding high amounts of peptide (~15-20 mg per 1 L culture) in a short time. The recombinant Ab(M1-42) forms characteristic aggregates similar to synthetic Ab42 aggregates as verified by western blots and atomic force microscopy to warrant future biological use. Our rapid, refined, and robust technique to purify human Ab(M1-42) can be used to synthesize chemical probes for several downstream in vitro and in vivo assays to facilitate AD research.


Author(s):  
Madhubhushan M ◽  
Seshaiah S ◽  
Chandrudu J ◽  
Sagar R ◽  
Akila CR

The common watermelon item (Citrullus lanatus) seeds were gained from castoffs verdant nourishments for use by decorating, sun ventilation and pulverizing. Light yellow-toned oil was gotten by dissolvable withdrawal using oil atmosphere and the going with traits were gotten using oil ether: pH, refractive rundown, thickness, dissolvable miscibility, coagulating temperature, fire nature, express gravity, streak point and warmth of consuming. With a shallow level of unsaturation, stepped level of smoothness, and proximity of raised degree of the sensible proportion of free unsaturated fats. The low assessment of the solidifying temperature of the oil offered a hint that the oil can be managed in various areas paying little heed to the qualification in temperature. The following level of linoleic destructive of the oil offers a hint of natural gradation of solidarity. Fatty esters are increasing expanding significance as a biodegradable swap for mineral oils. In some request regions, for example, cutting tool oil, gearbox, pressure-driven oils, and greases for raw petroleum creation, the oleochemical items are set up. Nonetheless, certain particular wellsprings of fatty esters are hitherto to be abused for this comparative reason. This exploration subsequently tests into one of the less used wellsprings of fatty esters in watermelon. The oil from the kernels demonstrations a top-notch yield presents significant utilitarian gatherings for change and thus was utilized to set up an assortment of oleochemicals which demonstrated excellence materials in contrast with the routinely utilized oils feed frameworks for oleochemicals after portrayal. The photopolymers acquired indicated piercing vinyl protons for consistent polymerize.


Author(s):  
F. Garnacho ◽  
J. Rovira ◽  
A. Khamlichi ◽  
P. Simon ◽  
T. Garcia ◽  
...  

Measurement ◽  
2021 ◽  
pp. 109674
Author(s):  
G. Crotti ◽  
D. Giordano ◽  
G. D'Avanzo ◽  
P.S. Letizia ◽  
M. Luiso

2014 ◽  
Vol 615 ◽  
pp. 57-62 ◽  
Author(s):  
Raquel Acero Cacho ◽  
Jose Antonio Albajez ◽  
José Antonio Yagüe-Fabra ◽  
Marta Torralba ◽  
Margarita Valenzuela ◽  
...  

The nanotechnology field has been developing strongly in recent years and ultra-precision measuring systems are nowadays required. A new two-dimensional moving platform with 50x50 mm range of travel, nanometer resolution and sub micrometer accuracy is being designed by the authors in order to be integrated with an Atomic Force Microscope (AFM). In this work the definition, design and experimental characterization of a homing sensor system for this 2D moving platform is presented. The homing sensor system will allow the generation of an absolute 2D reference for the platform (X-Y axis and θz rotation), defining an initial cero for the measuring system, which is based on laser encoders.


1994 ◽  
Vol 37 (5 Sup.) ◽  
Author(s):  
E. Pettinelli ◽  
N. Pierdicca ◽  
S. Piro ◽  
L. Versino

Detection and location of buried structures using the electromagnetic impulsive methodologies (GPR) require the study of the spatial distribution of energy irradiated by an antenna into the ground and the mechanisms of wave propagation and scattering from relevant targets. Evaluation of the difference in wave field distribution in the ground with respect to free space can provide some useful indications on the propagation of the Geo-radar signal in the ground and the spatial resolution capability of the GPR method. For this reason, a research group, involving “La Sapienza” University, Rome and the National Research Council began, during 1992, to perform studies on antenna radiation pattern, the propagation and scattering phenomena of GPR. This paper presents the experimental set up and the obtained results on the antenna radiation pattern.


Author(s):  
Märt-Erik Mäeots ◽  
Byungjin Lee ◽  
Andrea Nans ◽  
Seung-Geun Jeong ◽  
Mohammad M. N. Esfahani ◽  
...  

AbstractMechanistic understanding of biochemical reactions requires structural and kinetic characterization of the underlying chemical processes. However, no single experimental technique can provide this information in a broadly applicable manner and thus structural studies of static macromolecules are often complemented by biophysical analysis. Moreover, the common strategy of utilizing mutants or crosslinking probes to stabilize otherwise short-lived reaction intermediates is prone to trapping off-pathway artefacts and precludes determining the order of molecular events. To overcome these limitations and allow visualisation of biochemical processes at near-atomic spatial resolution and millisecond time scales, we developed a time-resolved sample preparation method for cryo-electron microscopy (trEM). We integrated a modular microfluidic device, featuring a 3D-mixing unit and a delay line of variable length, with a gas-assisted nozzle and motorised plunge-freeze set-up that enables automated, fast, and blot-free sample vitrification. This sample preparation not only preserves high-resolution structural detail but also substantially improves protein distribution across the vitreous ice. We validated the method by examining the formation of RecA filaments on single-stranded DNA. We could reliably visualise reaction intermediates of early filament growth across three orders of magnitude on sub-second timescales. Quantification of the trEM data allowed us to characterize the kinetics of RecA filament growth. The trEM method reported here is versatile, easy to reproduce and thus readily adaptable to a broad spectrum of fundamental questions in biology.


Signo ◽  
2016 ◽  
Vol 41 (70) ◽  
pp. 29 ◽  
Author(s):  
Jean-Remi Lapaire

Speakers are moving cognizers who engage in bodily acts of conceptualization. The “globe gesture” is among the most spectacular forms of “manual thinking” (Streeck 2009) used in formal talk. A characterization of the kinesic action typical of the “globe gesture” is first provided that shows how “the image of a bounded, supportable object” is created (McNeill 1992) and set up in gesture space. As conceptual objects are created and masses of semantic substance fashioned, visible shape is given to shapeless mental representations. A powerful semiotic trick is performed with a simple cognitive artifact. Interestingly, a willing suspension of disbelief is required of speakers and listeners who must temporarily give up their rational conceptions of visibility, materiality and palpability to watch the symbolic manipulation of invisible objects. The basic expressive properties of the “globe gesture” are next characterized: outlining and isolating objects of conception; neutralizing semantic specification; establishing a joint focus of attention and imagination; shaping, displaying and unifying content; creating a sense of reality and existence through physical presence. Iconic modifications of the standard metaphoric hand configuration, virtuosic elaborations and creative blends are finally examined before reporting the results of an experimental study of the globe gesture’s heuristic properties in a controlled environment. 14 students attending a multimodal “kineflective” seminar used the hand configuration to engage in “choreographic thinking” (Forsythe 2009) and develop a haptic understanding of derivation, nominalization, substantivation, conceptual reification. The globe gesture acted as a facilitator so long as a high degree of generality was maintained but was promptly discarded when words with a strong emotional appeal were introduced (e.g. sadness, madness). Emblems and iconic gestures were spontaneously performed instead.


2015 ◽  
Vol 35 (14) ◽  
pp. 3853-3861 ◽  
Author(s):  
Camille Gazeau ◽  
Jean Gillibert ◽  
Eric Blond ◽  
Pierre-Marie Geffroy ◽  
Nicolas Richet

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