Characterisation of Antibodies Against the Active Conformation of Gαi1 Using the SRU-BIND® Label-Free Detection System

2011 ◽  
pp. 255-268
Author(s):  
Melanie Leveridge ◽  
Chun-Wa Chung ◽  
Trevor Wattam
Keyword(s):  
Detection System ◽  
Active Conformation ◽  
Label Free ◽  
Label Free Detection

A DNAzyme based label-free detection system for miniaturized assays

2011 ◽  
Vol 7 (10) ◽  
pp. 2882 ◽  
Author(s):  
Daniela M. Köster ◽  
David Haselbach ◽  
Hans Lehrach ◽  
Harald Seitz
Keyword(s):  
Detection System ◽  
Label Free ◽  
Label Free Detection

scholarly journals Toward Development of a Label-Free Detection Technique for Microfluidic Fluorometric Peptide-Based Biosensor Systems

Micromachines ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 691
Author(s):  
Nikita Sitkov ◽  
Tatiana Zimina ◽  
Alexander Kolobov ◽  
Vladimir Karasev ◽  
Alexander Romanov ◽  
...  
Keyword(s):  
Troponin I ◽  
Troponin T ◽  
Detection System ◽  
Label Free ◽  
Protein Markers ◽  
Peptide Aptamers ◽  
Registration System ◽  
Biosensor System ◽  
Label Free Detection ◽  
Selection Of

The problems of chronic or noncommunicable diseases (NCD) that now kill around 40 million people each year require multiparametric combinatorial diagnostics for the selection of effective treatment tactics. This could be implemented using the biosensor principle based on peptide aptamers for spatial recognition of corresponding protein markers of diseases in biological fluids. In this paper, a low-cost label-free principle of biomarker detection using a biosensor system based on fluorometric registration of the target proteins bound to peptide aptamers was investigated. The main detection principle considered includes the re-emission of the natural fluorescence of selectively bound protein markers into a longer-wavelength radiation easily detectable by common charge-coupled devices (CCD) using a specific luminophore. Implementation of this type of detection system demands the reduction of all types of stray light and background fluorescence of construction materials and aptamers. The latter was achieved by careful selection of materials and design of peptide aptamers with substituted aromatic amino acid residues and considering troponin T, troponin I, and bovine serum albumin as an example. The peptide aptamers for troponin T were designed in silico using the «Protein 3D» (SPB ETU, St. Petersburg, Russia) software. The luminophore was selected from the line of ZnS-based solid-state compounds. The test microfluidic system was arranged as a flow through a massive of four working chambers for immobilization of peptide aptamers, coupled with the optical detection system, based on thick film technology. The planar optical setup of the biosensor registration system was arranged as an excitation-emission cascade including 280 nm ultraviolet (UV) light-emitting diode (LED), polypropylene (PP) UV transparent film, proteins layer, glass filter, luminophore layer, and CCD sensor. A laboratory sample has been created.

Low-Volume Label-Free Detection of Molecule-Protein Interactions on Microarrays by Imaging Reflectometric Interferometry

2016 ◽  
Vol 22 (4) ◽  
pp. 437-446 ◽  
Author(s):  
Juergen Burger ◽  
Christin Rath ◽  
Johannes Woehrle ◽  
Philipp A. Meyer ◽  
Nessim Ben Ammar ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Interaction Analysis ◽  
Detection System ◽  
Random Noise ◽  
Label Free ◽  
Baseline Drift ◽  
Flow Cells ◽  
System Sensitivity ◽  
Microfluidic Flow ◽  
Label Free Detection

This system allows the high-throughput protein interaction analysis on microarrays. We apply the interference technology 1λ–imaging reflectometric interferometry (iRIf) as a label-free detection method and create microfluidic flow cells in microscope slide format for low reagent consumption and lab work compatibility. By now, most prominent for imaging label-free interaction analyses on microarrays are imaging surface plasmon resonance (SPR) methods, quartz crystal microbalance, or biolayer interferometry. SPR is sensitive against temperature drifts and suffers from plasmon crosstalk, and all systems lack array size (maximum 96 spots). Our detection system is robust against temperature drifts. Microarrays are analyzed with a spatial resolution of 7 µm and time resolution of ≤50 fps. System sensitivity is competitive, with random noise of <5 × 10−5 and baseline drift of <3 × 10−6. Currently available spotting technologies limit array sizes to ~4 spots/mm2 (1080 spots/array); our detection system would allow ~40 spots/mm2 (10,800 spots/array). The microfluidic flow cells consist of structured PDMS inlays sealed by versatilely coated glass slides immobilizing the microarray. The injection protocol determines reagent volumes, priming rates, and flow cell temperatures for up to 44 reagents; volumes of ≤300 µL are validated. The system is validated physically by the biotinylated bovine serum albumin streptavidin assay and biochemically by thrombin aptamer interaction analysis, resulting in a KD of ~100 nM.

scholarly journals A large size-selective DNA nanopore with sensing applications

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Rasmus P. Thomsen ◽  
Mette Galsgaard Malle ◽  
Anders Hauge Okholm ◽  
Swati Krishnan ◽  
Søren S.-R. Bohr ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Single Molecule ◽  
Detection System ◽  
Optical Methods ◽  
Label Free ◽  
Sensing Applications ◽  
Large Size ◽  
Transmission Electron ◽  
Label Free Detection ◽  
External Signals

AbstractTransmembrane nanostructures like ion channels and transporters perform key biological functions by controlling flow of molecules across lipid bilayers. Much work has gone into engineering artificial nanopores and applications in selective gating of molecules, label-free detection/sensing of biomolecules and DNA sequencing have shown promise. Here, we use DNA origami to create a synthetic 9 nm wide DNA nanopore, controlled by programmable, lipidated flaps and equipped with a size-selective gating system for the translocation of macromolecules. Successful assembly and insertion of the nanopore into lipid bilayers are validated by transmission electron microscopy (TEM), while selective translocation of cargo and the pore mechanosensitivity are studied using optical methods, including single-molecule, total internal reflection fluorescence (TIRF) microscopy. Size-specific cargo translocation and oligonucleotide-triggered opening of the pore are demonstrated showing that the DNA nanopore can function as a real-time detection system for external signals, offering potential for a variety of highly parallelized sensing applications.

Low-Cost Label-Free Bio-Detection System Using Double-Sided Grating Waveguide Couplers

2015 ◽  
Author(s):  
Yi-Fan Ku ◽  
Hsun-Yuan Li ◽  
Yu-Chung Lin ◽  
Wen-Hsin Hsieh ◽  
Guo-En Chang
Keyword(s):  
Real Time ◽  
Detection System ◽  
Light Emitting Diode ◽  
Low Cost ◽  
Cost Effective ◽  
High Refractive Index ◽  
Optical Intensity ◽  
Label Free ◽  
Light Emitting ◽  
Label Free Detection

Low-cost label-free bio-sensing systems have long been desired to enable rapid, sensitive, quantitative, and high-throughput biosensing for bio-medical and chemical applications. Here we present an optical bio-detection system consists of injection-molded biosensors based on double-sided grating waveguide couplers and an optical intensity-based detection platform for low-cost, real-time, and label-free biosensing. The biosensors were fabricated combining injection-molding and sputtering techniques, providing unique advantages of low-cost and reduced production time. A simple and cost-effective optical intensity-based detection system employing a low-cost light emitting diode and a simple photodetector is also developed to perform label-free biosensing. We demonstrate that a high refractive index resolution of 6.43 × 10−5 RIU is achieved with this compact bio-sensing system, showing great promises for low-cost, real-time, label-free detection in bio-medical and chemical applications.

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