Quantification and Characterization of Atherosclerotic Lesions in Mice

2013 ◽  
pp. 320-331
Author(s):  
Abhinav Agarwal ◽  
Millicent G. Winner ◽  
Srinivas D. Sithu ◽  
Sanjay Srivastava
Keyword(s):  
Atherosclerotic Lesions

scholarly journals Characterization of smooth muscle cells from human atherosclerotic lesions and their responses to Notch signaling

2018 ◽  
Vol 99 (3) ◽  
pp. 290-304 ◽  
Author(s):  
Jessica Davis-Knowlton ◽  
Jacqueline E. Turner ◽  
Anna Turner ◽  
Sydney Damian-Loring ◽  
Nicholas Hagler ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Notch Signaling ◽  
Muscle Cells ◽  
Atherosclerotic Lesions

scholarly journals Isolation and Characterization of Foam Cells in Rabbit Atherosclerotic Lesions

1986 ◽  
Vol 14 (5) ◽  
pp. 1061-1063
Author(s):  
Michitaka NAITO ◽  
Masafumi KUZUYA ◽  
Chiaki FUNAKI ◽  
Kazuaki SHIBATA ◽  
Yasukazu NAKAYAMA ◽  
...  
Keyword(s):  
Foam Cells ◽  
Atherosclerotic Lesions ◽  
Isolation And Characterization

scholarly journals Immunochemical characterization of fibrinogen, fibrin I, and fibrin II in human thrombi and atherosclerotic lesions

Blood ◽  
1987 ◽  
Vol 69 (4) ◽  
pp. 1038-1045 ◽  
Author(s):  
A Bini ◽  
J Jr Fenoglio ◽  
J Sobel ◽  
J Owen ◽  
M Fejgl ◽  
...  
Keyword(s):  
Amino Acid ◽  
Total Protein ◽  
Cyanogen Bromide ◽  
Fibrinogen Concentration ◽  
Beta Chain ◽  
Amino Terminal ◽  
Atherosclerotic Lesions ◽  
Predominant Component

Abstract Arterial thrombi and atherosclerotic lesions were analyzed immunochemically and examined histologically. The extent of in vivo proteolytic cleavage of the amino-terminal end of fibrinogen by thrombin and plasmin was determined and quantitated by specific radioimmunoassays. The samples were treated with cyanogen bromide (CNBr), and the total amount of fibrinogen and fibrin-derived protein was determined as NDSK, the NH2-terminal disulfide knot of fibrinogen. Thrombin-releasable fibrinopeptides A and B were used to quantitate fibrinogen and fibrin I. Previous plasmin cleavage of the B beta chain was inferred from the amount of B beta 1–42 and B beta 15–42 in undigested NDSK. The results obtained in both acute and organized thrombi indicate that approximately 60% of the total protein (as determined by amino acid analysis) was fibrinogen-derived and that 70% to 80% of the fibrinogen-derived material was fibrin II. These findings support the hypothesis that fibrin II as distinct from fibrin I is the predominant component in a thrombus. In samples from normal and atherosclerotic aortas, fibrinogen-derived protein comprised less than 10% of the total protein. Samples from grossly normal aortas contained only fibrinogen and fibrin I. Fibrinogen concentration decreased and fibrin II concentration increased with increasing severity of the lesions, suggesting that increased fibrin II formation is associated with progression of atheromas.

scholarly journals Quantitative Analysis and Characterization of Atherosclerotic Lesions in the Murine Aortic Sinus

10.3791/50933 ◽  
2013 ◽  
Author(s):  
Daniel E. Venegas-Pino ◽  
Nicole Banko ◽  
Mohammed I. Khan ◽  
Yuanyuan Shi ◽  
Geoff H. Werstuck
Keyword(s):  
Quantitative Analysis ◽  
Aortic Sinus ◽  
Atherosclerotic Lesions

Characterization of Nucleating Proteolipids from Calcified and Non-Calcified Atherosclerotic Lesions

1990 ◽  
pp. 251-262 ◽  
Author(s):  
Rosemarie Romeo ◽  
Joan M. Augustyn ◽  
Gretchen Mandel ◽  
Assaad S. Daoud
Keyword(s):  
Atherosclerotic Lesions

scholarly journals Coronary Atherosclerotic Plaque Vulnerability Rather than Stenosis Predisposes to Non-ST Elevation Acute Coronary Syndromes

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Mohamed Laimoud ◽  
Farouk Faris ◽  
Helmy Elghawaby
Keyword(s):  
Stable Angina ◽  
Acute Coronary Syndromes ◽  
Syntax Score ◽  
Atherosclerotic Lesions ◽  
Group I ◽  
Coronary Syndromes ◽  
St Elevation ◽  
Coronary Lesions ◽  
Group Ii

Background. Non-ST elevation acute coronary syndromes (NSTE-ACS) may arise from moderately stenosed atherosclerotic lesions that suddenly undergo transformation to vulnerable plaques complicated by rupture and thrombosis. Objective. Assessment and tissue characterization of the coronary atherosclerotic lesions among NSTE-ACS patients compared to those with stable angina. Methodology. Evaluation of IVUS studies of 312 coronary lesions was done by 2 different experienced IVUS readers, 216 lesions in 66 patients with NSTE-ACS (group I) versus 96 lesions in 50 patients with stable angina (group II). Characterization of coronary plaques structure was done using colored-coded iMap technique. Results. The Syntax score was significantly higher in group I compared to group II (18.7 ± 7.8 vs. 8.07 ± 2.5, p=0.001). Body mass index (BMI) was significantly higher in group II while triglycerides levels were higher in group I (P=0.01 & P=0.04, respectively). History of previous MI and PCI was significantly higher in group I (P=0.016 & P=0.001, respectively). The coronary lesions of NSTE-ACS patients had less vessel area (9.86 ± 3.8 vs 11.36 ± 2.9, p=0.001), stenosis percentage (54.7 ± 14.9% vs 68.6 ± 8.7%, p=0.001), and plaque burden (54.4 ± 14.7 vs 67.8 ± 9.8, p=0.001) with negative remodeling index (0.95 ± 20 vs 1.02 ± 0.14, p=0.008) compared to the stable angina group. On the other hand, they had more lipid content (21.8 ± 7.03% vs 7.26 ± 3.47%, p=0.001), necrotic core (18.08 ± 10.19% vs 15.83 ± 4.9%, p=0.02), and calcifications (10.4 ± 5.2% vs 4.19 ± 3.29%, p=0.001) while less fibrosis (51.67 ± 7.07% vs 70.37 ± 11.7%, p=0.001) compared to the stable angina patients. Syntax score and core composition especially calcification and lipid content were significant predictors to NSTE-ACS. Conclusions. The vulnerability rather than the stenotic severity is the most important factor that predisposes to non-ST segment elevation acute coronary syndromes. The vulnerability is related to the lesion characteristics especially lipidic core and calcification while lesion fibrosis favours lesion stability.

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