scholarly journals Point‐of‐Care Platform for Rapid Multiplexed Detection of SARS‐CoV‐2 Variants and Respiratory Pathogens

2022 ◽  
pp. 2101013
Author(s):  
Alexander Y. Trick ◽  
Fan‐En Chen ◽  
Liben Chen ◽  
Pei‐Wei Lee ◽  
Alexander C. Hasnain ◽  
...  
Keyword(s):  
Point Of Care ◽  
Respiratory Pathogens ◽  
Multiplexed Detection

scholarly journals Multiplexed Reverse Transcriptase PCR Assay for Identification of Viral Respiratory Pathogens at the Point of Care

2007 ◽  
Vol 45 (11) ◽  
pp. 3498-3505 ◽  
Author(s):  
S. E. Letant ◽  
J. I. Ortiz ◽  
L. F. Bentley Tammero ◽  
J. M. Birch ◽  
R. W. Derlet ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Point Of Care ◽  
Respiratory Pathogens ◽  
Pcr Assay ◽  
Reverse Transcriptase Pcr ◽  
Viral Respiratory

scholarly journals Risk of bias and limits of reporting in diagnostic accuracy studies for commercial point-of-care tests for respiratory pathogens

2018 ◽  
Vol 146 (6) ◽  
pp. 747-756
Author(s):  
J.M. Hughes ◽  
C. Penney ◽  
S. Boyd ◽  
P. Daley
Keyword(s):  
Diagnostic Accuracy ◽  
Test Performance ◽  
Point Of Care ◽  
Risk Of Bias ◽  
Financial Impact ◽  
Test Accuracy ◽  
Respiratory Pathogens ◽  
Study Results ◽  
Group A ◽  
Point Of Care Tests

AbstractCommercial point-of-care (POC) diagnostic tests for Group A Streptococcus, Streptococcus pneumoniae, and influenza virus have large potential diagnostic and financial impact. Many published reports on test performance, often funded by diagnostics companies, are prone to bias. The Standards for Reporting of Diagnostic Accuracy (STARD 2015) are a protocol to encourage accurate, transparent reporting. The Quality Assessment of Diagnostic Accuracy Studies (QUADAS-2) tool evaluates risk of bias and transportability of results. We used these tools to evaluate diagnostic test accuracy studies of POC studies for three respiratory pathogens. For the 96 studies analysed, compliance was <25% for 14/34 STARD 2015 standards, and 3/7 QUADAS-2 domains showed a high risk of bias. All reports lacked reporting of at least one criterion. These biases should be considered in the interpretation of study results.

Towards a point-of-care nanoplasmonic biosensor for rapid and multiplexed detection of pathogenic infections

2018 ◽  
Author(s):  
Maria Soler Aznar ◽  
Xiaokang Li ◽  
Alexander Belushkin ◽  
Hatice Altug ◽  
Filiz Yesilköy
Keyword(s):  
Point Of Care ◽  
Multiplexed Detection

scholarly journals Rapid Multiplexed Detection on Lateral-Flow Devices Using a Laser Direct-Write Technique

Biosensors ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 97 ◽  
Author(s):  
Peijun He ◽  
Ioannis Katis ◽  
Robert Eason ◽  
Collin Sones
Keyword(s):  
Bacterial Infections ◽  
Point Of Care ◽  
Simultaneous Detection ◽  
Cross Reactivity ◽  
Lateral Flow ◽  
Direct Write ◽  
Multiplexed Detection ◽  
Amyloid A ◽  
Flow Devices ◽  
Laser Direct Write

Paper-based lateral flow devices (LFDs) are regarded as ideal low-cost diagnostic solutions for point-of-care (POC) scenarios that allow rapid detection of a single analyte within a fluidic sample, and have been in common use for a decade. In recent years, there has been an increasing need for rapid and simultaneous detection of multiple analytes present within a single sample and to facilitate this, we report here a novel solution—detection using a multi-path LFD created via the precise partitioning of the single flow-path of a standard LFD using our previously reported laser direct-write (LDW) technique. The multiple flow-paths allow the simultaneous detection of the different analytes individually within each of the parallel channels without any cross-reactivity. The appearance of coloured test lines in individual channels indicates the presence of the different analytes within a sample. We successfully present the use of a LDW-patterned multi-path LFD for multiplexed detection of a biomarker panel comprising C-reactive protein (CRP) and Serum amyloid A-1 (SAA1), used for the diagnosis of bacterial infections. Overall, we demonstrate the use of our LDW technique in the creation of a novel LFD that enables multiplexed detection of two inflammation markers within a single LFD providing a detection protocol that is comparatively more efficient than the standard sequential multiplexing procedure.

A hydrogel sensor-based microfluidic platform for the quantitative and multiplexed detection of fertility markers for point-of-care immunoassays

2019 ◽  
Vol 11 (12) ◽  
pp. 1639-1650 ◽  
Author(s):  
Satish Kalme ◽  
Srinivasan Kandaswamy ◽  
Anusha Chandrasekharmath ◽  
Reeta Katiyar ◽  
Gokul Prasath Rajamanickam ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Point Of Care ◽  
Microfluidic Platform ◽  
Multiplexed Detection ◽  
Hydrogel Particle ◽  
Multiplexed Immunoassay ◽  
Porous Hydrogel

We report a new point-of-care, multiplexed immunoassay platform based on 3D porous hydrogel particle sensors embedded into a plastic microfluidic device.

scholarly journals Multiplexed detection of respiratory pathogens with a portable analyzer in a “raw-sample-in and answer-out” manner

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Nan Li ◽  
Minjie Shen ◽  
Jiajia Liu ◽  
Li Zhang ◽  
Huili Wang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Limit Of Detection ◽  
Solid Phase ◽  
Bacterial Species ◽  
Magnetic Bead ◽  
Acid Extraction ◽  
Respiratory Pathogens ◽  
Nucleic Acid Extraction ◽  
Multiplexed Detection ◽  
Portable Analyzer

AbstractCoronavirus disease 2019 (COVID-19) has emerged, rapidly spread and caused significant morbidity and mortality worldwide. There is an urgent public health need for rapid, sensitive, specific, and on-site diagnostic tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. In this study, a fully integrated and portable analyzer was developed to detect SARS-CoV-2 from swab samples based on solid-phase nucleic acid extraction and reverse transcription loop-mediated isothermal amplification (RT-LAMP). The swab can be directly inserted into a cassette for multiplexed detection of respiratory pathogens without pre-preparation. The overall detection process, including swab rinsing, magnetic bead-based nucleic acid extraction, and 8-plex real-time RT-LAMP, can be automatically performed in the cassette within 80 min. The functionality of the cassette was validated by detecting the presence of a SARS-CoV-2 pseudovirus and three other respiratory pathogens, i.e., Klebsiella pneumoniae, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia. The limit of detection (LoD) for the SARS-CoV-2 pseudovirus was 2.5 copies/μL with both primer sets (N gene and ORF1ab gene), and the three bacterial species were successfully detected with an LoD of 2.5 colony-forming units (CFU)/μL in 800 μL of swab rinse. Thus, the analyzer developed in this study has the potential to rapidly detect SARS-CoV-2 and other respiratory pathogens on site in a “raw-sample-in and answer-out” manner.

Companion and Point-of-Care Sensor System for Rapid Multiplexed Detection of a Panel of Infectious Disease Markers

2017 ◽  
pp. 247263031769677
Author(s):  
Anjan Panneer Selvam ◽  
Shalini Prasad
Keyword(s):  
Whole Blood ◽  
Dynamic Range ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Lipoteichoic Acid ◽  
Impedance Change ◽  
Label Free ◽  
Nylon Membrane ◽  
Multiplexed Detection

A nanochannel-based electrochemical biosensor has been demonstrated for rapid and multiplexed detection of a panel of three biomarkers associated with rapid detection of sepsis. The label-free biosensor detected procalcitonin (PCT), lipoteichoic acid (LTA), and lipopolysaccharide (LPS) from human whole blood. The biosensor comprises a nanoporous nylon membrane integrated onto a microelectrode sensor platform for nanoconfinement effects. Charge perturbations due to biomarker binding are recorded as impedance changes using electrochemical impedance spectroscopy. The measured impedance change is used to quantitatively determine the concentration of the three biomarkers using antibody receptors from the tested sample. We were successful in detecting and quantifying the three biomarkers from whole blood. The limit of detection was 0.1 ng/mL for PCT and 1 µg/mL for LPS and LTA. The sensor was able to demonstrate a dynamic range of detection from 01.1 ng/mL to 10 µg/mL for PCT and from 1 µg/mL to 1000 µg/mL for LPS and LTA biomarkers. This novel technology has promising preliminary results toward the design of sensors for rapid and sensitive detection of the three panel biomarkers in whole blood toward diagnosis and classification of sepsis.

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