A hydrogel sensor-based microfluidic platform for the quantitative and multiplexed detection of fertility markers for point-of-care immunoassays

2019 ◽  
Vol 11 (12) ◽  
pp. 1639-1650 ◽  
Author(s):  
Satish Kalme ◽  
Srinivasan Kandaswamy ◽  
Anusha Chandrasekharmath ◽  
Reeta Katiyar ◽  
Gokul Prasath Rajamanickam ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Point Of Care ◽  
Microfluidic Platform ◽  
Multiplexed Detection ◽  
Hydrogel Particle ◽  
Multiplexed Immunoassay ◽  
Porous Hydrogel

We report a new point-of-care, multiplexed immunoassay platform based on 3D porous hydrogel particle sensors embedded into a plastic microfluidic device.

scholarly journals Direct and rapid measurement of hydrogen peroxide in human blood using a microfluidic device

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
R. Gaikwad ◽  
P. R. Thangaraj ◽  
A. K. Sen
Keyword(s):  
Hydrogen Peroxide ◽  
Microfluidic Device ◽  
Human Blood ◽  
Blood Cells ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Time Interval ◽  
Fluorescence Measurement ◽  
Rapid Measurement ◽  
Automated Method

AbstractThe levels of hydrogen peroxide ($${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 ) in human blood is of great relevance as it has emerged as an important signalling molecule in a variety of disease states. Fast and reliable measurement of $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 levels in the blood, however, continues to remain a challenge. Herein we report an automated method employing a microfluidic device for direct and rapid measurement of $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 in human blood based on laser-induced fluorescence measurement. Our study delineates the critical factors that affect measurement accuracy—we found blood cells and soluble proteins significantly alter the native $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 levels in the time interval between sample withdrawal and detection. We show that separation of blood cells and subsequent dilution of the plasma with a buffer at a ratio of 1:6 inhibits the above effect, leading to reliable measurements. We demonstrate rapid measurement of $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 in plasma in the concentration range of 0–49 µM, offering a limit of detection of 0.05 µM, a sensitivity of 0.60 µM−1, and detection time of 15 min; the device is amenable to the real-time measurement of $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 in the patient’s blood. Using the linear correlation obtained with known quantities of $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 , the endogenous $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 concentration in the blood of healthy individuals is found to be in the range of 0.8–6 µM. The availability of this device at the point of care will have relevance in understanding the role of $${\mathrm{H}}_{2}{\mathrm{O}}_{2}$$ H 2 O 2 in health and disease.

A Thread/Fabric-Based Band as A Flexible and Wearable Microfluidic Device for Sweat Sensing and Monitoring

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Zhiqi Zhao ◽  
Qiujin Li ◽  
Linna Chen ◽  
Yu Zhao ◽  
Jixian Gong ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Point Of Care ◽  
Monitoring Systems ◽  
Biological Analytes

Flexible biosensors for monitoring systems have emerged as a promising portable diagnostics platform due to their potential for in situ point-of-care (POC) analytic devices. Assessment of biological analytes in sweat...

scholarly journals New portable smartphone-based PDMS microfluidic kit for the simultaneous colorimetric detection of arsenic and mercury

RSC Advances ◽  
2018 ◽  
Vol 8 (48) ◽  
pp. 27091-27100 ◽  
Author(s):  
Abbas Motalebizadeh ◽  
Hasan Bagheri ◽  
Sasan Asiaei ◽  
Nasim Fekrat ◽  
Abbas Afkhami
Keyword(s):  
Gold Nanoparticles ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Point Of Care ◽  
Colorimetric Detection ◽  
Microfluidic Platform

A smartphone-based microfluidic platform was developed for point-of-care (POC) detection using surface plasmon resonance (SPR) of gold nanoparticles (GNPs).

scholarly journals Microfluidic on-demand droplet generation, storage, retrieval, and merging for single-cell pairing

Lab on a Chip ◽  
2019 ◽  
Vol 19 (3) ◽  
pp. 493-502 ◽  
Author(s):  
Hesam Babahosseini ◽  
Tom Misteli ◽  
Don L. DeVoe
Keyword(s):  
Single Cell ◽  
Aqueous Phase ◽  
Microfluidic Device ◽  
Droplet Generation ◽  
Microfluidic Platform ◽  
On Demand

A multifunctional microfluidic platform combining on-demand aqueous-phase droplet generation, multi-droplet storage, and controlled merging of droplets selected from a storage library in a single integrated microfluidic device is described.

scholarly journals Electroosmotic flow driven microfluidic device for bacteria isolation using magnetic microbeads

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Samuel Miller ◽  
Alison A. Weiss ◽  
William R. Heineman ◽  
Rupak K. Banerjee
Keyword(s):  
Microfluidic Device ◽  
Electroosmotic Flow ◽  
Point Of Care ◽  
Food Poisoning ◽  
Fluorescent Intensity ◽  
Magnetic Microbeads ◽  
E Coli ◽  
Detection Assay ◽  
Flow Capture ◽  
Improved Performance

Abstract The presence of bacterial pathogens in water can lead to severe complications such as infection and food poisoning. This research proposes a point-of-care electroosmotic flow driven microfluidic device for rapid isolation and detection of E. coli in buffered solution (phosphate buffered saline solution). Fluorescent E. coli bound to magnetic microbeads were driven through the microfluidic device using both constant forward flow and periodic flow switching at concentrations ranging from 2 × 105 to 4 × 107 bacteria/mL. A calibration curve of fluorescent intensity as a function of bacteria concentration was created using both constant and switching flow, showing an increase in captured fluorescent pixel count as concentration increases. In addition, the use of the flow switching resulted in a significant increase in the capture efficiency of E. coli, with capture efficiencies up to 83% ± 8% as compared to the constant flow capture efficiencies (up to 39% ± 11%), with a sample size of 3 µL. These results demonstrate the improved performance associated with the use of the electroosmotic flow switching system in a point-of-care bacterial detection assay.

A microfluidic device for the batch adsorption of a protein on adsorbent particles

The Analyst ◽  
2017 ◽  
Vol 142 (19) ◽  
pp. 3656-3665 ◽  
Author(s):  
Hoon Suk Rho ◽  
Alexander Thomas Hanke ◽  
Marcel Ottens ◽  
Han Gardeniers
Keyword(s):  
Adsorption Isotherm ◽  
Microfluidic Device ◽  
Batch Adsorption ◽  
Single Experiment ◽  
Microfluidic Platform

A microfluidic platform or “microfluidic batch adsorption device” is presented, which performs two sets of 9 parallel protein incubations with/without adsorbent particles to achieve an adsorption isotherm of a protein in a single experiment.

Towards a point-of-care nanoplasmonic biosensor for rapid and multiplexed detection of pathogenic infections

2018 ◽  
Author(s):  
Maria Soler Aznar ◽  
Xiaokang Li ◽  
Alexander Belushkin ◽  
Hatice Altug ◽  
Filiz Yesilköy
Keyword(s):  
Point Of Care ◽  
Multiplexed Detection

scholarly journals Ultrasensitive multi-species detection of CRISPR-Cas9 by a portable centrifugal microfluidic platform

2019 ◽  
Vol 11 (5) ◽  
pp. 559-565 ◽  
Author(s):  
Christopher R. Phaneuf ◽  
Kyle J. Seamon ◽  
Tyler P. Eckles ◽  
Anchal Sinha ◽  
Joseph S. Schoeniger ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Microfluidic Device ◽  
Clinical Sample ◽  
Microfluidic Platform ◽  
Species Detection

Combined activity- and immunoassays for CRISPR/Cas9 on a portable microfluidic device with integrated sample preparation from clinical sample matrices.

Graphene Protein Field Effect Biomedical Sensor for Glucose Measurements

2015 ◽  
Vol 1725 ◽  
Author(s):  
Dorian Liepmann ◽  
Kiana Aran ◽  
Pulickel M. Ajayan ◽  
Sowmya Viswanathan ◽  
Pingzuo Li ◽  
...  
Keyword(s):  
Glucose Oxidase ◽  
Microfluidic Device ◽  
Field Effect ◽  
Silicon Oxide ◽  
Point Of Care ◽  
Oxide Surface ◽  
Medical Sensors ◽  
Improved Performance ◽  
Biosensor Chip ◽  
Initial Results

ABSTRACTThe need for improved medical sensors based on lab-on-a-chip technologies has increased significantly because of the dramatic growth in the number of people with chronic diseases and the associated costs for their healthcare. Development and initial results of a hybrid plastic microfluidic device with an integrated graphene-protein biosensor chip for use in point-of-care (POC) is described. The initial prototype is a glucometer that uses optimized glucose oxidase bound to a graphene field effect sensor. Technologies required for development of the prototype include modification of the glucose oxidase for improved performance by protein engineering, methods to bind the enzyme to the graphene attached to the silicon oxide surface of sensor chip, and integration into a thermoplastic microfluidic device. Initial results indicate the prototype glucometer can measure glucose concentrations from low physiological levels to molar concentrations.

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