Printing machinery’s reliability is one of the most important index parameter and good dynamic characteristics are essential for high speed printing press. The dynamic characteristic is predictable for printing machine with dynamic design. With the complexity of printing technique and printing machine, information, such as load on components is lack, leading to the difficulty of dynamic model solving and further more the dynamic parameters needs to be verified. Firstly, according to the dynamic design method, a dynamic design process based on measuring information for printing machine was put forward. Secondly, on the basis of principle and structure analysis to typical printing machine, dynamic characteristics measurement item and method was determined. With a wallboard of a two-color offset press acting as an example, the utilizing method was illustrated through dynamic modeling, theoretical model analysis, experiment model analysis based on vibration measurement and comparing the theoretical and experimental results. Finally, a Dynamic Design Assistant Platform based Vibration Measurement for Printing Machine was developed and had been used in 10 kinds of printing machine measurement and analysis. The conclusion shows that the tolerance of the theoretical model analysis results and experiment results is allowed. A new approach for new printing machine design and optimal design for existed printing machine is given.