Differential nucleolus organizer activity in normal and leukemic bone marrow

The nucleolar organizer activity in several Aegilops × rye hybrids (A. triuncialis × Secale cereale, A. variabilis × S. cereale, A. biunicialis × S. cereale, A. biuncialis × S. vavilovii, A. juvenalis × S. cereale) is analyzed by using a highly reproducible silver-staining procedure. The 1U and 5U chromosomes show a strong nucleolar activity, suppressing the NOR activity of chromosome 1R from rye in all the hybrid combinations (UCR, USvR, UMbR, UMbRv, and UMjDR). The nucleolus organizer chromosomes from the genomes C, Sv, Mb, and Mj show small activities. Our results confirm previous data of the nucleolar organizer activity predominant status of the 1U and 5U chromosomes from the U genome (A. umbellulata) and the weakest condition of the 1R chromosome from rye. A diagram showing the relationships between the nucleolar organizer activities of chromosomes from different genomes of Triticeae is presented.Key words: nucleolar competition, amphiplasty, Ag-NORs, Triticeae, Aegilops, Secale.

Download Full-text

Suppression of human nucleolus organizer activity in mouse-human somatic hybrid cells

Experimental Cell Research ◽

10.1016/0014-4827(76)90373-6 ◽

1976 ◽

Vol 101 (2) ◽

pp. 235-243 ◽

Cited By ~ 334

Author(s):

D.A. Miller ◽

V.G. Dev ◽

R. Tantravahi ◽

O.J. Miller

Keyword(s):

Somatic Hybrid ◽

Nucleolus Organizer ◽

Hybrid Cells ◽

Organizer Activity

Download Full-text

Glycogen in guinea pig neutrophils: Ultrastructural study of neutrophils at the intradermal site of BCG injection

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100077207 ◽

1983 ◽

Vol 41 ◽

pp. 726-727

Author(s):

Corazon D. Bucana

Keyword(s):

Bone Marrow ◽

Guinea Pig ◽

Electron Density ◽

Peritoneal Cavity ◽

Ultrastructural Study ◽

Guinea Pigs ◽

Random Distribution ◽

Glycogen Content ◽

Polymorphonuclear Neutrophils ◽

Ultrastructural Studies

In the circulating blood of man and guinea pigs, glycogen occurs primarily in polymorphonuclear neutrophils and platelets. The amount of glycogen in neutrophils increases with time after the cells leave the bone marrow, and the distribution of glycogen in neutrophils changes from an apparently random distribution to large clumps when these cells move out of the circulation to the site of inflammation in the peritoneal cavity. The objective of this study was to further investigate changes in glycogen content and distribution in neutrophils. I chose an intradermal site because it allows study of neutrophils at various stages of extravasation.Initially, osmium ferrocyanide and osmium ferricyanide were used to fix glycogen in the neutrophils for ultrastructural studies. My findings confirmed previous reports that showed that glycogen is well preserved by both these fixatives and that osmium ferricyanide protects glycogen from solubilization by uranyl acetate.I found that osmium ferrocyanide similarly protected glycogen. My studies showed, however, that the electron density of mitochondria and other cytoplasmic organelles was lower in samples fixed with osmium ferrocyanide than in samples fixed with osmium ferricyanide.

Download Full-text

Mammalian Bone Marrow Acetylcholinesterase

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100053024 ◽

1982 ◽

Vol 40 ◽

pp. 44-45

Author(s):

Ezzatollah Keyhani

Keyword(s):

Central Nervous System ◽

Bone Marrow ◽

Nervous System ◽

Common Property ◽

Extracellular Medium ◽

The Central Nervous System ◽

The Common ◽

Mammalian Bone

Acetylcholinesterase (EC 3.1.1.7) (ACHE) has been localized at cholinergic junctions both in the central nervous system and at the periphery and it functions in neurotransmission. ACHE was also found in other tissues without involvement in neurotransmission, but exhibiting the common property of transporting water and ions. This communication describes intracellular ACHE in mammalian bone marrow and its secretion into the extracellular medium.

Download Full-text

The structure of pre-mRNA and mRNA from erythroid cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100082030 ◽

1978 ◽

Vol 36 (2) ◽

pp. 234-235

Author(s):

A.-M. Ladhoff ◽

B.J. Thiele ◽

Ch. Coutelle ◽

S. Rosenthal

Keyword(s):

Bone Marrow ◽

Structural Transformation ◽

Electron Micrographs ◽

Ammonium Acetate ◽

Bone Marrow Cells ◽

Erythroid Cells ◽

Globin Mrna ◽

Ordered Structures ◽

Rabbit Bone ◽

Rabbit Reticulocytes

The suggested precursor-product relationship between the nuclear pre-mRNA and the cytoplasmic mRNA has created increased interest also in the structure of these RNA species. Previously we have been published electron micrographs of individual pre-mRNA molecules from erythroid cells. An intersting observation was the appearance of a contour, probably corresponding to higher ordered structures, on one end of 10 % of the pre-mRNA molecules from erythroid rabbit bone marrow cells (Fig. 1A). A virtual similar contour was observed in molecules of 9S globin mRNA from rabbit reticulocytes (Fig. 1B). A structural transformation in a linear contour occurs if the RNA is heated for 10 min to 90°C in the presence of 80 % formamide. This structural transformation is reversible when the denatured RNA is precipitated and redissolved in 0.2 M ammonium acetate.

Download Full-text