scholarly journals Single‐cell profiling of circulating and brain‐resident immune cells in a mouse model for amyloidosis and in aged mice

2020 ◽  
Vol 16 (S2) ◽  
Author(s):  
Lynn van Olst ◽  
Jan Verhoeff ◽  
Sjoerd T.T. Schetters ◽  
Lianne A. Hulshof ◽  
Roland van Dijk ◽  
...  
Keyword(s):  
Mouse Model ◽  
Single Cell ◽  
Immune Cells ◽  
Aged Mice ◽  
Single Cell Profiling

scholarly journals Single-Cell Profiling to Explore Immunological Heterogeneity of Tumor Microenvironment in Breast Cancer

2021 ◽  
Vol 12 ◽  
Author(s):  
Xiao Yuan ◽  
Jinxi Wang ◽  
Yixuan Huang ◽  
Dangang Shangguan ◽  
Peng Zhang
Keyword(s):  
Breast Cancer ◽  
Tumor Microenvironment ◽  
Single Cell ◽  
Immune Cells ◽  
Critical Role ◽  
Therapy Resistance ◽  
Spatial Profiling ◽  
Wide Range ◽  
Single Cell Profiling ◽  
Immunological Heterogeneity

Immune infiltrates in the tumor microenvironment (TME) of breast cancer (BRCA) have been shown to play a critical role in tumorigenesis, progression, invasion, and therapy resistance, and thereby will affect the clinical outcomes of BRCA patients. However, a wide range of intratumoral heterogeneity shaped by the tumor cells and immune cells in the surrounding microenvironment is a major obstacle in understanding and treating BRCA. Recent progress in single-cell technologies such as single-cell RNA sequencing (scRNA-seq), mass cytometry, and digital spatial profiling has enabled the detailed characterization of intratumoral immune cells and vastly improved our understanding of less-defined cell subsets in the tumor immune environment. By measuring transcriptomes or proteomics at the single-cell level, it provides an unprecedented view of the cellular architecture consist of phenotypical and functional diversities of tumor-infiltrating immune cells. In this review, we focus on landmark studies of single-cell profiling of immunological heterogeneity in the TME, and discuss its clinical applications, translational outlook, and limitations in breast cancer studies.

scholarly journals Single-cell profiling of dynamic cytokine secretion and the phenotype of immune cells

PLoS ONE ◽  
2017 ◽  
Vol 12 (8) ◽  
pp. e0181904 ◽  
Author(s):  
Xingyue An ◽  
Victor G. Sendra ◽  
Ivan Liadi ◽  
Balakrishnan Ramesh ◽  
Gabrielle Romain ◽  
...  
Keyword(s):  
Single Cell ◽  
Immune Cells ◽  
Cytokine Secretion ◽  
Single Cell Profiling

scholarly journals Single-cell profiling of immune system alterations in lymphoid, barrier and solid tissues in aged mice

Nature Aging ◽  
2021 ◽  
Author(s):  
Sinduya Krishnarajah ◽  
Florian Ingelfinger ◽  
Ekaterina Friebel ◽  
Dilay Cansever ◽  
Ana Amorim ◽  
...  
Keyword(s):  
Immune System ◽  
Single Cell ◽  
Aged Mice ◽  
Single Cell Profiling ◽  
Solid Tissues

scholarly journals Immune landscape of a genetically-engineered murine model of glioma relative to human glioma by single-cell sequencing

2020 ◽  
Author(s):  
Daniel B. Zamler ◽  
Takashi Shingu ◽  
Laura M. Kahn ◽  
Cynthia Kassab ◽  
Martina Ott ◽  
...  
Keyword(s):  
Mouse Model ◽  
Single Cell ◽  
Human Disease ◽  
Human Tumor ◽  
Myeloid Cell ◽  
Genetically Engineered ◽  
List Type ◽  
Knockout Mouse Model ◽  
Mouse Tumors ◽  
Single Cell Profiling

SUMMARYBackgroundThis study focuses on the evaluation of intratumoral immune infiltrates of the Qk/trp53/Pten (QPP) triple-knockout mouse model of glioblastoma with the purpose of establishing its relevance compared to the human disease. This included an analysis at the single cell level, of immune cells composition in both spontaneous and implanted mouse tumors and of samples obtained from human tumor resections.MethodsWe analyzed a cohort of fifteen spontaneous QPP mice, nine implanted QPP mice and 10 glioma patients by standard immune profiling methods such as IHC. Of those, we analyzed three spontaneous QPP mice, three implanted QPP mice, and 10 glioma patients by single cell RNA sequencing.ResultsIn the QPP samples we identified a predominantly myeloid cell population of monocytes, macrophages, and microglia, with minor populations of T, B, and NK cells. When comparing spontaneous and implanted mouse samples, we found that there were more neutrophil, T and NKT cells in the implanted model. In human samples, most of the lymphoid and myeloid compartments were immunosuppressive. While the complexity of the myeloid cell populations is preserved between the QPP model and the human disease, we observed significantly more immune-stimulatory populations in the implanted mouse model.ConclusionsWe found that, despite differences at the subpopulation level, the immunosuppressive nature of the immune system in glioma, is recapitulated in our mouse model. Although we observed differences in the proportions of immune infiltrates derived from the spontaneous and implanted mouse models and in LGG compared to GBM, the major constituents are present in all cases and these differences may be caused by various etiological or pathogenic influences on tumor-immune interactions.Key PointsNew GBM mouse model for immunotherapySingle cell profiling of the immune systems in human and mouse GBM

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