scholarly journals Direct Electromembrane Extraction based Mass Spectrometry: A Tool for Studying Drug Metabolism Properties of Liver Organoids

2021 ◽  
Author(s):  
Frøydis Sved Skottvoll ◽  
Aleksandra Aizenshtadt ◽  
Frederik Hansen ◽  
Mikel Amirola Martinez ◽  
Jannike Mørch Andersen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Drug Metabolism ◽  
Electromembrane Extraction ◽  
Liver Organoids

On-chip electromembrane extraction for monitoring drug metabolism in real time by electrospray ionization mass spectrometry

The Analyst ◽  
2012 ◽  
Vol 137 (14) ◽  
pp. 3321 ◽  
Author(s):  
Nickolaj Jacob Petersen ◽  
Jacob Sønderby Pedersen ◽  
Nicklas Nørgård Poulsen ◽  
Henrik Jensen ◽  
Christian Skonberg ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Drug Metabolism ◽  
Electrospray Ionization ◽  
Real Time ◽  
Electrospray Ionization Mass Spectrometry ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Monitoring Drug ◽  
Electromembrane Extraction ◽  
On Chip

Development and characterization of a small electromembrane extraction probe coupled with mass spectrometry for real-time and online monitoring of in vitro drug metabolism

2013 ◽  
Vol 406 (2) ◽  
pp. 421-429 ◽  
Author(s):  
Helene Bonkerud Dugstad ◽  
Nickolaj Jacob Petersen ◽  
Henrik Jensen ◽  
Charlotte Gabel-Jensen ◽  
Steen Honoré Hansen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Drug Metabolism ◽  
Real Time ◽  
Online Monitoring ◽  
Electromembrane Extraction

scholarly journals Electromembrane Extraction and Mass Spectrometry for Liver Organoid Drug Metabolism Studies

2021 ◽  
Vol 93 (7) ◽  
pp. 3576-3585
Author(s):  
Frøydis Sved Skottvoll ◽  
Frederik André Hansen ◽  
Sean Harrison ◽  
Ida Sneis Boger ◽  
Ago Mrsa ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Drug Metabolism ◽  
Electromembrane Extraction

scholarly journals Electromembrane extraction and mass spectrometry for liver organoid drug metabolism studies

2020 ◽  
Author(s):  
Frøydis Sved Skottvoll ◽  
Frederik Hansen ◽  
Sean Harrison ◽  
Ida Sneis Boger ◽  
Ago Mrsa ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Phase I ◽  
Toxicity Screening ◽  
Drug Metabolites ◽  
Medium Components ◽  
Electromembrane Extraction ◽  
Ultrahigh Performance Liquid Chromatography ◽  
Liver Organoids ◽  
Cell Medium

AbstractLiver organoids are emerging tools for precision drug development and toxicity screening. We demonstrate that electromembrane extraction (EME) based on electrophoresis across an oil membrane is suited for segregating selected organoid-derived drug metabolites prior to mass spectrometry (MS)-based measurements. EME, allowed drugs and drug metabolites to be separated from cell medium components (albumin, etc.) that could interfere with subsequent measurements. Multi-well EME (Parallel-EME) holding 100 μL solutions allowed for simple and repeatable monitoring of heroin phase I metabolism kinetics. Organoid Parallel-EME extracts were compatible with ultrahigh-performance liquid chromatography (UHPLC) used to separate the analytes prior to detection. Taken together, liver organoids are well-matched with EME followed by MS-based measurements.

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