Specially elaborated thermally induced phase separation to fabricate poly(L-lactic acid) scaffolds with ultra large pores and good interconnectivity

2006 ◽  
Vol 101 (5) ◽  
pp. 3336-3342 ◽  
Author(s):  
Yihong Gong ◽  
Zuwei Ma ◽  
Changyou Gao ◽  
Wei Wang ◽  
Jiacong Shen
Keyword(s):  
Lactic Acid ◽  
Phase Separation ◽  
Thermally Induced Phase Separation ◽  
Thermally Induced

Structure–property relation of porous poly (l-lactic acid) scaffolds fabricated using organic solvent mixtures and controlled cooling rates and its bio-compatibility with human adipose stem cells

2018 ◽  
Vol 33 (4) ◽  
pp. 397-415 ◽  
Author(s):  
Harish Chinnasami ◽  
Jeff Gimble ◽  
Ram V Devireddy
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Lactic Acid ◽  
Phase Separation ◽  
Mechanical Strength ◽  
Separation Method ◽  
Thermally Induced Phase Separation ◽  
Cooling Rates ◽  
Thermally Induced ◽  
Pore Sizes

Thermally induced phase separation method was used to make porous three-dimensional poly (l-lactic acid) scaffolds. The effect of imposed thermal profile during freezing of the poly (l-lactic acid) in dioxane solution on the scaffold was characterized by their micro-structure, porosity (%), pore sizes’ distribution, and mechanical strength. The porosity (%) decreased considerably with increasing concentrations of poly (l-lactic acid) in the solution, while a decreasing trend was observed with increasing cooling rates. The mechanical strength increases with increase in poly (l-lactic acid) concentration and also with increase in the cooling rate for both types of solvents. Therefore, mechanical strength was increased by higher cooling rates while the porosity (%) remained relatively consistent. Scaffolds made using higher concentrations of poly (l-lactic acid; 7% and 10% w/v) in solvent showed better mechanical strength which improved relatively with increasing cooling rates (1°C–40°C/min). This phenomenon of enhanced structural integrity with increasing cooling rates was more prominent in scaffolds made from higher initial poly (l-lactic acid) concentrations. Human adipose–derived stem cells were cultured on these scaffold (7% and 10% w/v) prepared by thermally induced phase separation at all cooling rates to measure the cell proliferation efficiency as a function of their micro-structural properties. Mean pore sizes played a crucial role in cell proliferation than percent porosity since all scaffolds were >88% porous. The viability percent of human adipose tissue–derived adult stem cells increased consistently with longer periods of culture. Thus, poly (l-lactic acid) scaffolds prepared by thermally controlled thermally induced phase separation method could be a prime candidate for making ex vivo tissue-engineered grafts for surgical implantation.

Poly lactic acid based foams prepared via thermally induced phase separation (TIPS): A method to tune the crystallinity

2012 ◽  
Author(s):  
Francesco Carfì Pavia ◽  
Vincenzo La Carrubba ◽  
Valerio Brucato
Keyword(s):  
Lactic Acid ◽  
Phase Separation ◽  
Poly Lactic Acid ◽  
Thermally Induced Phase Separation ◽  
Thermally Induced

Free poly(l-lactic acid) spherulites grown from thermally induced phase separation and crystallization kinetics

2014 ◽  
Vol 52 (22) ◽  
pp. 1476-1489 ◽  
Author(s):  
Ruilai Liu ◽  
Kaina Li ◽  
Min Liu ◽  
Yingying Liu ◽  
Haiqing Liu
Keyword(s):  
Lactic Acid ◽  
Phase Separation ◽  
Crystallization Kinetics ◽  
Thermally Induced Phase Separation ◽  
Thermally Induced

Preparation of PLLA/HAP/β-TCP Composite Scaffold for Bone Tissue Engineering

2014 ◽  
Vol 513-517 ◽  
pp. 143-146 ◽  
Author(s):  
Xue Jun Wang ◽  
Tao Lou ◽  
Jing Yang ◽  
Zhen Yang ◽  
Kun Peng He
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Lactic Acid ◽  
Phase Separation ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Composite Scaffold ◽  
Thermally Induced Phase Separation ◽  
Composite Scaffolds ◽  
Thermally Induced

In this study, a nanofibrous poly (L-lactic acid) (PLLA) scaffold reinforced by Hydroxyapatite (HAP) and β-tricalcium phosphate (β-TCP) was fabricated using the thermally induced phase separation method. The composite scaffold morphology showed a nanofibrous PLLA matrix and evenly distributed β-TCP/HAP particles. The composite scaffold had interconnective micropores and the pore size ranged 2-10 μm. Introducing β-TCP/HAP particles into PLLA matrix significantly improved the mechanical properties of the composite scaffold. In summary, the new composite scaffolds show a great deal promise for use in bone tissue engineering.

Osteo-Induction of Human Adipose Derived Stem Cells Cultured on Poly (L-Lactic Acid) Scaffolds Prepared by Thermally Induced Phase Separation Method

2015 ◽  
Author(s):  
H. Chinnasami ◽  
R. Devireddy
Keyword(s):  
Stem Cells ◽  
Compressive Strength ◽  
Lactic Acid ◽  
Phase Separation ◽  
Pore Size ◽  
Adipose Derived Stem Cells ◽  
Thermally Induced Phase Separation ◽  
Thermally Induced ◽  
Bio Compatibility ◽  
Cells Cultured

Bio-degradable Poly (l-lactic acid) (PLLA) scaffolds synthesized using thermally induced phase separation (TIPS) method was used to load cryo-preserved human adipose derived stem cells (hASCs). To make the scaffolds, PLLA-Dioxane solutions were formed by dissolving PLLA in 1,4-Dioxane with three different compositions (wt/vol). These PLLA-Dioxane solutions, frozen in three different cooling rates were lyophilized at 0.037bar and −70°C for 48hrs resulting in porous PLLA scaffolds. Based on the porosity, pore size and compressive strength, a suitable scaffold was chosen to investigate its bio-compatibility and osteo-inductive potential.

Poly(L-lactic acid) Microfiltration Membrane Formation via Thermally Induced Phase Separation with Drying

2011 ◽  
Vol 44 (7) ◽  
pp. 467-475 ◽  
Author(s):  
Takaaki Tanaka ◽  
Masatou Ueno ◽  
Youhei Watanabe ◽  
Tomoaki Kouya ◽  
Masayuki Taniguchi ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Phase Separation ◽  
Thermally Induced Phase Separation ◽  
Membrane Formation ◽  
Thermally Induced ◽  
Microfiltration Membrane
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