Electrospun polylactide/silk fibroin-gelatin composite tubular scaffolds for small-diameter tissue engineering blood vessels

2009 ◽  
Vol 113 (4) ◽  
pp. 2675-2682 ◽  
Author(s):  
Shudong Wang ◽  
Youzhu Zhang ◽  
Guibo Yin ◽  
Hongwei Wang ◽  
Zhihui Dong
Keyword(s):  
Tissue Engineering ◽  
Blood Vessels ◽  
Silk Fibroin ◽  
Small Diameter

Scaffolds in tissue engineering of blood vessels

2010 ◽  
Vol 88 (9) ◽  
pp. 855-873 ◽  
Author(s):  
Divya Pankajakshan ◽  
Devendra K. Agrawal
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Blood Vessels ◽  
Vascular Tissue ◽  
Small Diameter ◽  
Biological Scaffolds ◽  
Hemodynamic Forces ◽  
Biodegradable Scaffolds

Tissue engineering of small diameter (<5 mm) blood vessels is a promising approach for developing viable alternatives to autologous vascular grafts. It involves in vitro seeding of cells onto a scaffold on which the cells attach, proliferate, and differentiate while secreting the components of extracellular matrix that are required for creating the tissue. The scaffold should provide the initial requisite mechanical strength to withstand in vivo hemodynamic forces until vascular smooth muscle cells and fibroblasts reinforce the extracellular matrix of the vessel wall. Hence, the choice of scaffold is crucial for providing guidance cues to the cells to behave in the required manner to produce tissues and organs of the desired shape and size. Several types of scaffolds have been used for the reconstruction of blood vessels. They can be broadly classified as biological scaffolds, decellularized matrices, and polymeric biodegradable scaffolds. This review focuses on the different types of scaffolds that have been designed, developed, and tested for tissue engineering of blood vessels, including use of stem cells in vascular tissue engineering.

3D Coculture of Human Endothelial Cells and Myofibroblasts for Vascular Tissue Engineering

2007 ◽  
Author(s):  
Rolf A. A. Pullens ◽  
Maria Stekelenburg ◽  
Carlijn V. C. Bouten ◽  
Frank P. T. Baaijens ◽  
Mark J. Post
Keyword(s):  
Tissue Engineering ◽  
Blood Vessels ◽  
Vascular Tissue ◽  
Artery Bypass ◽  
Small Diameter ◽  
Life Time ◽  
Mechanical Conditioning ◽  
Tissue Properties ◽  
Vein Grafts ◽  
Limited Life

Cardiovascular disease is still the number one cause of death in the industrialized world. Diseased small diameter blood vessels are frequently replaced by native grafts. However, these vessels have a limited life time [1], for example the patency at 10 year after coronary artery bypass grafting of saphenous vein grafts is 57% [2]. Tissue engineering (TE) of small diameter blood vessels seems a promising approach to overcome these shortcomings or address the increasing need for substitutes during follow up surgery. Mechanical conditioning of myofibroblast (MFs) seeded constructs appears to be beneficial for functional tissue properties, such as cell proliferation, ECM production and mechanical strength [3,4]. Without a functional endothelial cell (ECs) layer however, patency may be compromised by thrombogenecity. Construction of an EC layer might on the other hand affect the tissue composition during culture, as was shown for bovine ECs, which influenced proliferation and ECM production of smooth muscle cells [5].

Collagen-Silk Fibroin Fibers: A Promising Scaffold for Vascular Tissue Engineering

2012 ◽  
Vol 706-709 ◽  
pp. 572-577
Author(s):  
Estelle Paternotte ◽  
Mariana Agostini de Moraes ◽  
Marisa Masumi Beppu ◽  
D. Mantovani
Keyword(s):  
Tissue Engineering ◽  
Silk Fibroin ◽  
Vascular Tissue ◽  
Small Diameter ◽  
Vascular Tissue Engineering ◽  
Research Teams ◽  
Cellular Attachment ◽  
New Models ◽  
Vascular Engineering ◽  
Vascular Replacement

Small caliber vascular replacement (<4 mm) still remains a challenge for medical and research teams, as no available vascular substitutes (VS) are suitable for small diameter bypass. Vascular engineering proposes new models of small diameter VS but rare are those that meet the biocompatibility and mechanical criteria. In this study, we developed a new scaffold made by the combination of two natural biomacromolecules: collagen and silk fibroin. The scaffold was further cellularised with porcine smooth muscle cells. First, the behavior of cells in the collagen-fibroin constructs was verified in order to evaluate the biocompatibility of the scaffold with the cells. Then, gel mass loss and cellular attachment, morphology, spreading and viability were analysed. The results showed an excellent interaction and biocompatibility between collagen, silk fibroin fibers and cells. Thus, the collagen-fibroin construct appears to be a very attractive material for vascular tissue engineering.

Porous hybrid structures based on P(DLLA-co-TMC) and collagen for tissue engineering of small-diameter blood vessels

2006 ◽  
Vol 79B (2) ◽  
pp. 425-434 ◽  
Author(s):  
Laura Buttafoco ◽  
Niels P. Boks ◽  
Paula Engbers-Buijtenhuijs ◽  
Dirk W. Grijpma ◽  
Andre A. Poot ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Blood Vessels ◽  
Small Diameter ◽  
Hybrid Structures

Production of the biomimetic small diameter blood vessels for cardiovascular tissue engineering

2018 ◽  
Vol 68 (5) ◽  
pp. 243-255 ◽  
Author(s):  
Mehmet Onur Aydogdu ◽  
Joshua Chou ◽  
Esra Altun ◽  
Nazmi Ekren ◽  
Selami Cakmak ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Blood Vessels ◽  
Small Diameter ◽  
Cardiovascular Tissue ◽  
Cardiovascular Tissue Engineering

Mesenchymal stem cell-based tissue engineering of small-diameter blood vessels

Vascular ◽  
2011 ◽  
Vol 19 (4) ◽  
pp. 206-213 ◽  
Author(s):  
Jian-De Dong ◽  
Jin-Hong Huang ◽  
Feng Gao ◽  
Zhao-Hui Zhu ◽  
Jian Zhang
Keyword(s):  
Tissue Engineering ◽  
Blood Vessels ◽  
Pulsatile Flow ◽  
Ex Vivo ◽  
Flow Direction ◽  
Small Diameter ◽  
Luminal Surface ◽  
Novel Approach ◽  
Tissue Engineered Blood Vessels ◽  
Von Willebrand

The aim of the study was to construct small-diameter vascular grafts using canine mesenchymal stem cells (cMSCs) and a pulsatile flow bioreactor. cMSCs were isolated from canine bone marrow and expanded ex vivo. cMSCs were then seeded onto the luminal surface of decellularized arterial matrices, which were further cultured in a pulsatile flow bioreactor for four days. Immunohistochemical staining and scanning electron microscopy was performed to characterize the tissue-engineered blood vessels. cMSCs were successfully seeded onto the luminal surface of porcine decellularized matrices. After four-day culture in the pulsatile flow bioreactor, the cells were highly elongated and oriented to the flow direction. Immunohistochemistry demonstrated that the cells cultured under pulsatile flow expressed Von Willebrand factor, an endothelial cell marker. In conclusion, cMSCs seeded onto decellularized arterial matrices could differentiate into endothelial lineage after culturing in a pulsatile flow bioreactor, which provides a novel approach for tissue engineering of small-diameter blood vessels.

First steps towards tissue engineering of small-diameter blood vessels: Preparation of flat scaffolds of collagen and elastin by means of freeze drying

2006 ◽  
Vol 77B (2) ◽  
pp. 357-368 ◽  
Author(s):  
L. Buttafoco ◽  
P. Engbers-Buijtenhuijs ◽  
A. A. Poot ◽  
P. J. Dijkstra ◽  
W. F. Daamen ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Blood Vessels ◽  
Freeze Drying ◽  
Small Diameter

scholarly journals Blood vessel replacement: 50 years of development and tissue engineering paradigms in vascular surgery

2009 ◽  
pp. S119-S140 ◽  
Author(s):  
J Chlupáč ◽  
E Filová ◽  
L Bačáková
Keyword(s):  
Tissue Engineering ◽  
Blood Vessels ◽  
Vascular Graft ◽  
Healing Process ◽  
Small Diameter ◽  
Engineering Technology ◽  
Vascular Prostheses ◽  
Standard Material ◽  
Vein Graft Disease ◽  
Graft Disease

The gold standard material in bypass surgery of blood vessels remains the patient’s own artery or vein. However, this material may be unavailable, or may suffer vein graft disease. Currently available vascular prostheses, namely polyethylene terephthalate (PET, Dacron) and expanded polytetrafluoroethylene (ePTFE), perform well as large-caliber replacements, but their long-term patency is discouraging in small-caliber applications (<6 mm), such as in coronary, crural or microvessel surgery. This failure is mainly a result of an unfavorable healing process with surface thrombogenicity, due to lack of endothelial cells and anastomotic intimal hyperplasia caused by hemodynamic disturbances. An ideal small-diameter vascular graft has become a major focus of research. Novel biomaterials have been manufactured, and tissue-biomaterial interactions have been optimized. Tissue engineering technology has proven that the concept of partially or totally living blood vessels is feasible. The purpose of this review is to outline the vascular graft materials that are currently being implanted, taking into account cell-biomaterial physiology, tissue engineering approaches and the collective achievements of the authors.

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