Enhancement of RecET-mediated in vivo linear DNA assembly by a xonA mutation

Assembly of intact, replicating plasmids from linear DNA fragments introduced into bacterial cells, i.e. in vivo cloning, is a facile genetic engineering technology that avoids many of the problems associated with standard in vitro cloning. Here we report characterization of various parameters of in vivo linear DNA assembly mediated by either the RecET recombination system or the bacteriophage λ Red recombination system. As previously observed, RecET is superior to Red for this reaction when the terminal homology is 50 bases. Deletion of the E. coli xonA gene, encoding Exonuclease I, a 3′→5′ single-strand DNA exonuclease, substantially improves the efficiency of in vivo linear DNA assembly for both systems. Deletion of ExoI function allowed robust RecET assembly of six DNA segments to create a functional plasmid. The linear DNAs are joined accurately with very few errors. This discovery provides a significant improvement to previously reported in vivo linear DNA assembly technologies.

Native and Engineered Probiotics: Promising Agents against Related Systemic and Intestinal Diseases

Intestinal homeostasis is a dynamic balance involving the interaction between the host intestinal mucosa, immune barrier, intestinal microecology, nutrients, and metabolites. Once homeostasis is out of balance, it will increase the risk of intestinal diseases and is also closely associated with some systemic diseases. Probiotics (Escherichia coli Nissle 1917, Akkermansia muciniphila, Clostridium butyricum, lactic acid bacteria and Bifidobacterium spp.), maintaining the gut homeostasis through direct interaction with the intestine, can also exist as a specific agent to prevent, alleviate, or cure intestinal-related diseases. With genetic engineering technology advancing, probiotics can also show targeted therapeutic properties. The aims of this review are to summarize the roles of potential native and engineered probiotics in oncology, inflammatory bowel disease, and obesity, discussing the therapeutic applications of these probiotics.

Computer-Based Electronic Engineering Technology

Most of the current image fusion algorithms directly process the original image, neglect the analysis of the main components of the image, and have a great influence on the effect of image fusion. In this paper, the main component analysis method is used to decompose the image, divided into low rank matrix and sparse matrix, introduced compression perception technology and NSST transformation algorithm to process the two types of matrix, according to the corresponding fusion rules to achieve image fusion, through experimental results: this algorithm has greater mutual information compared with traditional algorithms, structural information similarity and average gradient.

Research on A Joint Attribute Based Neural Network Model for Multi-class Voltage Violation

Electronic engineering technology is a symbol of modern technology, and a large number of electronic devices have been produced. This paved the way for the development of the Internet. This article analyzes the characteristics of the application of computer electronic engineering technology, and it analyzes the measures to strengthen the application of electronic engineering technology and the measures to promote the development of electronic engineering technology. In addition, this article also studies the teaching system design of electronic engineering technology.

Innovation and Entrepreneurship for the Self-Reliant and Localized Economy

Innovation and entrepreneurship (I&E) are two closely related words that go hand in hand in this era. Innovation is about applying creativity for different engineering/technology/service problems and producing unique solutions, while entrepreneurship is about applying the same to bring ideas to life by making them feasible to work. It is also about doing the business work. In this chapter, the authors review how innovation and entrepreneurship help create a self-reliant and localized economy. The chapter also looks at the importance of introducing entrepreneurship and innovation to an academic curriculum. By this approach, universities can reduce the gap of introducing the I&E concepts and use their synergies with the engineering technology course contents to create an innovative mindset, thereby creating a self-reliant and localized economy.

Exploring the Teaching and Learning Indicators towards Education 4.0 in MTUN, Malaysia

MTUN has been given responsibility for producing skilled TVET workforces in the industrial sectors. Therefore, the education structure in MTUN is gradually making a transition to Education 4.0 to integrate the existing education structure with the discovery of the 4th Industrial Revolution new technologies. The impact of this shift demands changes in the implementation of teaching towards Education 4.0. Teaching towards Education 4.0 is a phenomenon in moving the change of MTUN to a higher level. However, there is an issue of a mismatch of skills between the industry's needs and graduates' quality to face the 4th Industrial Revolution in Malaysia. Therefore, this study explores how to implement Education 4.0 to produce quality and technologically skilled graduates required by the industry. Thus, this study aims to determine the indicators and elements of teaching towards Education 4.0 for Engineering Technology in MTUN. The study involved seven experts in instructional design technology and in Technical Vocational Education and Training (TVET). The interview data collections were analysed using thematic analysis to explain the subject matter of this study. Eight elements were identified to implement teaching towards Education 4.0. These include Industry 4.0 elements, courses design, projects, practical, simulation, educator readiness, up-skilling and re-skilling training, and industrial experience. These elements may be viewed in two mains constructs which are essential to implement in the Engineering Technology programme in MTUN. There are curriculum and educator professional development. Further studies, which take these variables into account will need to be undertaken.