A Recombinant Fragment of Human Surfactant Protein D Lacking the Short Collagen-Like Stalk Fails to Correct Morphological Alterations in Lungs of SP-D Deficient Mice

The commensal microbiota within the gastrointestinal tract is essential in maintaining homeostasis. Indeed, dysregulation in the repertoire of microbiota can result in the development of intestinal immune–inflammatory diseases. Further, this immune regulation by gut microbiota is important systemically, impacting health and disease of organ systems beyond the local environment of the gut. What has not been explored is how distant organs might in turn shape the microbiota via microbe-targeted molecules. Here, we provide evidence that surfactant protein D (SP-D) synthesized in the gallbladder and delivered into intestinal lumen binds selectively to species of gut commensal bacteria. SP-D–deficient mice manifest intestinal dysbiosis and show a susceptibility to dextran sulfate sodium-induced colitis. Further, fecal transfer from SP-D–deficient mice to wild-type, germ-free mice conveyed colitis susceptibility. Interestingly, colitis caused a notable increase inSftpdgene expression in the gallbladder, but not in the lung, via the activity of glucocorticoids produced in the liver. These findings describe a unique mechanism of interorgan regulation of intestinal immune homeostasis by SP-D with potential clinical implications such as cholecystectomy.

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Pulmonary dysfunction in neonatal SP-B-deficient mice

AJP Lung Cellular and Molecular Physiology ◽

10.1152/ajplung.1997.273.4.l875 ◽

1997 ◽

Vol 273 (4) ◽

pp. L875-L882 ◽

Cited By ~ 28

Author(s):

Keisuke Tokieda ◽

Jeffrey A. Whitsett ◽

Jean C. Clark ◽

Timothy E. Weaver ◽

Kazushige Ikeda ◽

...

Keyword(s):

Critical Role ◽

Protein A ◽

Surfactant Protein ◽

Lung Compliance ◽

Phospholipid Content ◽

Surfactant Protein D ◽

Intratracheal Administration ◽

Lung Expansion ◽

B Mice ◽

Deficient Mice

Pulmonary function was assessed in newborn wild-type and homozygous and heterozygous surfactant protein B (SP-B)-deficient mice after birth. SP-B+/+ and SP-B+/− mice became well oxygenated and survived postnatally. Although lung compliance was decreased slightly in the SP-B+/− mice, lung volumes and compliances were decreased markedly in homozygous SP-B−/− mice. They died rapidly after birth, failing to inflate their lungs or oxygenate. SP-B proprotein was absent in the SP-B−/− mice and was reduced in the SP-B+/− mice, as assessed by Western analysis. Surfactant protein A, surfactant proprotein C, surfactant protein D, and surfactant phospholipid content in lungs from SP-B+/− and SP-B−/− mice were not altered. Lung saturated phosphatidylcholine and precursor incorporation into saturated phosphatidylcholine were not influenced by SP-B genotype. Intratracheal administration of perfluorocarbon resulted in lung expansion, oxygenation, and prolonged survival of SP-B−/− mice and in reduced lung compliance in SP-B+/+ and SP-B+/− mice. Lack of SP-B caused respiratory failure at birth, and decreased SP-B protein was associated with reduced lung compliance. These findings demonstrate the critical role of SP-B in perinatal adaptation to air breathing.

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Selective Inhibition of Inducible NO Synthase Activity In Vivo Reverses Inflammatory Abnormalities in Surfactant Protein D-Deficient Mice

The Journal of Immunology ◽

10.4049/jimmunol.179.12.8090 ◽

2007 ◽

Vol 179 (12) ◽

pp. 8090-8097 ◽

Cited By ~ 28

Author(s):

Elena N. Atochina-Vasserman ◽

Michael F. Beers ◽

Helchem Kadire ◽

Yaniv Tomer ◽

Adam Inch ◽

...

Keyword(s):

Selective Inhibition ◽

Surfactant Protein ◽

No Synthase ◽

Surfactant Protein D ◽

Inducible No Synthase ◽

Deficient Mice

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Effect of irradiation/bone marrow transplantation on alveolar epithelial type II cells is aggravated in surfactant protein D deficient mice

Histochemistry and Cell Biology ◽

10.1007/s00418-016-1479-7 ◽

2016 ◽

Vol 147 (1) ◽

pp. 49-61 ◽

Cited By ~ 5

Author(s):

Christian Mühlfeld ◽

Jens Madsen ◽

Rose-Marie Mackay ◽

Jan Philipp Schneider ◽

Julia Schipke ◽

...

Keyword(s):

Bone Marrow ◽

Bone Marrow Transplantation ◽

Marrow Transplantation ◽

Surfactant Protein ◽

Surfactant Protein D ◽

Type Ii Cells ◽

Type Ii ◽

Alveolar Epithelial ◽

Deficient Mice

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Orphan designation: Recombinant fragment of human surfactant protein-D, Prevention of bronchopulmonary dysplasia

Case Medical Research ◽

10.31525/cmr-1a9567c ◽

2019 ◽

Author(s):

Keyword(s):

Bronchopulmonary Dysplasia ◽

Surfactant Protein ◽

Surfactant Protein D ◽

Recombinant Fragment ◽

Orphan Designation

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Efficient resolution of Pneumocystis murina infection in surfactant protein A-deficient mice following withdrawal of corticosteroid-induced immunosuppression

Journal of Medical Microbiology ◽

10.1099/jmm.0.46190-0 ◽

2006 ◽

Vol 55 (2) ◽

pp. 143-147 ◽

Cited By ~ 8

Author(s):

Michael Linke ◽

Alan Ashbaugh ◽

Judith Koch ◽

Reiko Tanaka ◽

Peter Walzer

Keyword(s):

Protein A ◽

Lavage Fluid ◽

Surfactant Protein ◽

Lung Lavage ◽

Surfactant Protein A ◽

Surfactant Protein D ◽

Wild Type ◽

Enhanced Expression ◽

Lymphocyte Populations ◽

Deficient Mice

Following withdrawal of immunosuppression, surfactant protein A (SP-A)-deficient and wild-type mice cleared Pneumocystis murina infection in a similar manner, but exhibited significant differences in lymphocyte populations, interleukin (IL)-6 levels and chemokine expression levels. A higher percentage of lymphocytes were detected in lung lavage fluid from SP-A-deficient mice, but more CD4+ T cells were isolated from lung tissue of wild-type mice. Higher concentrations of IL-6 were detected in lavage fluid and enhanced expression of lymphotactin and RANTES were detected in the lungs of wild-type mice. Equal levels of surfactant protein D were detected in SP-A-deficient and wild-type mice and no differences were detected in markers of lung injury between the two strains of mice. Thus, SP-A does not enhance organism clearance, but does modulate the host immune response during resolution of P. murina infection.

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