Pregabalin Alters Nociceptive Behavior and Expression Level of P2X3 Receptor in the Spinal Dorsal Horn in a Rat Model Induced by Chronic Compression of the Dorsal Root Ganglion

2013 ◽  
Vol 296 (12) ◽  
pp. 1907-1912 ◽  
Author(s):  
Jianfeng Yu ◽  
Peng Fu ◽  
Yan Zhang ◽  
Shuzhen Liu ◽  
Donghong Cui
Keyword(s):  
Dorsal Root Ganglion ◽  
Dorsal Horn ◽  
Rat Model ◽  
Dorsal Root ◽  
Spinal Dorsal Horn ◽  
Expression Level ◽  
P2x3 Receptor ◽  
Nociceptive Behavior ◽  
Spinal Dorsal

scholarly journals Neonatal vincristine administration modulates intrinsic neuronal excitability in the rat dorsal root ganglion and spinal dorsal horn during adolescence

Pain ◽  
2019 ◽  
Vol 160 (3) ◽  
pp. 645-657 ◽  
Author(s):  
Katie A. Schappacher ◽  
Wenrui Xie ◽  
Jun-Ming Zhang ◽  
Mark L. Baccei
Keyword(s):  
Dorsal Root Ganglion ◽  
Dorsal Horn ◽  
Dorsal Root ◽  
Spinal Dorsal Horn ◽  
Neuronal Excitability ◽  
Spinal Dorsal

Changes in pain behavior and glial activation in the spinal dorsal horn after pulsed radiofrequency current administration to the dorsal root ganglion in a rat model of lumbar disc herniation

2013 ◽  
Vol 19 (2) ◽  
pp. 256-263 ◽  
Author(s):  
Hee Kyung Cho ◽  
Yun Woo Cho ◽  
Eun Hyuk Kim ◽  
Menno E. Sluijter ◽  
Se Jin Hwang ◽  
...  
Keyword(s):  
Dorsal Horn ◽  
Lumbar Disc Herniation ◽  
Disc Herniation ◽  
Rat Model ◽  
Dorsal Root ◽  
Spinal Dorsal Horn ◽  
Lumbar Disc ◽  
Exposed Group ◽  
Pulsed Radiofrequency ◽  
Spinal Dorsal

Object Herniated discs can induce sciatica by mechanical compression and/or chemical irritation caused by proinflammatory cytokines. Using immunohistochemistry methods in the dorsal horn of a rat model of lumbar disc herniation, the authors investigated the effects of pulsed radiofrequency (PRF) current administration to the dorsal root ganglion (DRG) on pain-related behavior and activation of microglia, astrocytes, and mitogen-activated protein kinase. Methods A total of 33 Sprague-Dawley rats were randomly assigned to either a sham-operated group (n = 10) or a nucleus pulposus (NP)–exposed group (n = 23). Rats in the NP-exposed group were further subdivided into NP exposed with sham stimulation (NP+sham stimulation, n = 10), NP exposed with PRF (NP+PRF, n = 10), or euthanasia 10 days after NP exposure (n = 3). The DRGs in the NP+PRF rats were exposed to PRF waves (2 Hz) for 120 seconds at 45 V on postoperative Day 10. Rats were tested for mechanical allodynia 10 days after surgery and at 8 hours, 1 day, 3 days, 10 days, 20 days, and 40 days after PRF administration. Immunohistochemical staining of astrocytes (glial fibrillary acidic protein), microglia (OX-42), and phosphorylated extracellular signal–regulated kinases (pERKs) in the spinal dorsal horn was performed at 41 days after PRF administration. Results Starting at 8 hours after PRF administration, mechanical withdrawal thresholds dramatically increased; this response persisted for 40 days (p < 0.05). After PRF administration, immunohistochemical expressions of OX-42 and pERK in the spinal dorsal horn were quantitatively reduced (p < 0.05). Conclusions Pulsed radiofrequency administration to the DRG reduced mechanical allodynia and downregulated microglia activity and pERK expression in the spinal dorsal horn of a rat model of lumbar disc herniation.

RETRACTED: Effects of electro-acupuncture on NT-4 expression in spinal dorsal root ganglion and associated segments of the spinal dorsal horn in cats subjected to adjacent dorsal root ganglionectomy

2009 ◽  
Vol 450 (2) ◽  
pp. 158-162 ◽  
Author(s):  
Fen Liu ◽  
Wei-Wei Sun ◽  
Ying Wang ◽  
Li-Qun Hu ◽  
Ping Dai ◽  
...  
Keyword(s):  
Dorsal Root Ganglion ◽  
Dorsal Horn ◽  
Dorsal Root ◽  
Spinal Dorsal Horn ◽  
Spinal Dorsal

Reversal of neurochemical alterations in the spinal dorsal horn and dorsal root ganglia by Mas-related gene (Mrg) receptors in a rat model of spinal nerve injury

2016 ◽  
Vol 91 ◽  
pp. 274-283 ◽  
Author(s):  
Dongmei Wang ◽  
Yaping Xue ◽  
Yanhua Yan ◽  
Minjie Lin ◽  
Jiajia Yang ◽  
...  
Keyword(s):  
Dorsal Horn ◽  
Nerve Injury ◽  
Dorsal Root Ganglia ◽  
Rat Model ◽  
Dorsal Root ◽  
Spinal Dorsal Horn ◽  
Spinal Nerve ◽  
Related Gene ◽  
Spinal Dorsal

scholarly journals L-bupivacaine Inhibition of Nociceptive Transmission in Rat Peripheral and Dorsal Horn Neurons

2020 ◽  
Vol 134 (1) ◽  
pp. 88-102
Author(s):  
Daisuke Uta ◽  
Kohei Koga ◽  
Hidemasa Furue ◽  
Keiji Imoto ◽  
Megumu Yoshimura
Keyword(s):  
Dorsal Root Ganglion ◽  
Dorsal Horn ◽  
Dorsal Root ◽  
Spinal Dorsal Horn ◽  
Dorsal Root Ganglion Neurons ◽  
Evoked Responses ◽  
Half Maximum ◽  
Sodium Currents ◽  
Spinal Dorsal ◽  
Ganglion Neurons

Background Although the widely used single L-enantiomers of local anesthetics have less toxic effects on the cardiovascular and central nervous systems, the mechanisms mediating their antinociceptive actions are not well understood. The authors hypothesized that significant differences in the ion channel blocking abilities of the enantiomers of bupivacaine would be identified. Methods The authors performed electrophysiologic analysis on rat dorsal root ganglion neurons in vitro and on spinal transmissions in vivo. Results In the dorsal root ganglion, these anesthetics decreased the amplitudes of action potentials. The half-maximum inhibitory concentrations of D-enantiomer D-bupivacaine were almost equal for Aβ (29.5 μM), Aδ (29.7μM), and C (29.8 μM) neurons. However, the half-maximum inhibitory concentrations of L-bupivacaine was lower for Aδ (19.35 μM) and C (19.5 μM) neurons than for A β (79.4 μM) neurons. Moreover, D-bupivacaine almost equally inhibited tetrodotoxin-resistant (mean ± SD: 15.8 ± 10.9% of the control, n = 14, P &lt; 0.001) and tetrodotoxin-sensitive (15.4 ± 15.6% of the control, n = 11, P = 0.004) sodium currents. In contrast, L-bupivacaine suppressed tetrodotoxin-resistant sodium currents (26.1 ± 19.5% of the control, n = 18, P &lt; 0.001) but not tetrodotoxin-sensitive sodium currents (74.5 ± 18.2% of the control, n = 11, P = 0.477). In the spinal dorsal horn, L-bupivacaine decreased the area of pinch-evoked excitatory postsynaptic currents (39.4 ± 11.3% of the control, n = 7, P &lt; 0.001) but not touch-evoked responses (84.2 ± 14.5% of the control, n = 6, P = 0.826). In contrast, D-bupivacaine equally decreased pinch- and touch-evoked responses (38.8 ± 9.5% of the control, n = 6, P = 0.001, 42.9 ± 11.8% of the control, n = 6, P = 0.013, respectively). Conclusions These results suggest that the L-enantiomer of bupivacaine (L-bupivacaine) effectively inhibits noxious transmission to the spinal dorsal horn by blocking action potential conduction through C and Aδ afferent fibers. Editor’s Perspective What We Already Know about This Topic What This Article Tells Us That Is New

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