Evaluation of relative beam-profile-compensated quantum yield of upconverting nanoparticles over a wide dynamic range of power densities

The presented work uses a discrete strategy of beam profile compensation to evaluate the local internal quantum yield (iQY) of upconverting nanoparticles (UCNPs) at the pixel level of the beam...

SHERRY2: A method for rapid and sensitive single cell RNA-seq

Many single cell RNA-seq applications aim to probe a wide dynamic range of gene expression, but most of them are still challenging to accurately quantify low-aboundance transcripts. Based on our previous finding that Tn5 transposase can directly cut-and-tag DNA/RNA hetero-duplexes, we present SHERRY2, an optimized protocol for sequencing transcriptomes of single cells or single nuclei. SHERRY2 is robust and scalable, and it has higher sensitivity and more uniform coverage in comparison with prevalent scRNA-seq methods. With throughput of a few thousand cells per batch, SHERRY2 can reveal the subtle transcriptomic differences between cells and facilitate important biological discoveries.

TWO-DIMENSIONAL COLOUR APPEARANCE SCALES FOR COLOUR STIMULI HAVING HIGH DYNAMIC RANGE

New colour appearance scales close to daily experience and image quality enhancement are highly desired including whiteness, blackness, vividness and depth. This article describes a new experiment to accumulate the data under HDR (high dynamic range) conditions. The data were then used to test the performance of different colour appearance scales such as CIELAB and CAM16-UCS plus the recent extension by Berns’ Vab*, Dab*. The results showed those Berns’ scales gave reasonable performance. However, there was no scale capable of predicting colour appearance data covering a wide dynamic range. New scales were developed based on the absolute scales of brightness and colourfulness of CAM16-UCS and gave accurate prediction to the data.

High precision phase estimation with controllable sensitivity and dynamic range

High precision phase estimation is at the core of modern physics and practical applications. We investigate a method for high precision phase estimation via inserting a reference state which enables weak measurement technique to be used in wide dynamic range. A reference phase is introduced artificially to offset the time delay between preselection state and reference state. The sensitivity of measured phase and the linear dynamic range are controllable by adjusting reference phase. Moreover, an arbitrary postselection in measurement is applicable by choosing appropriate reference phase. This method has merits of controllable sensitivity and wide dynamic range, which shows great potential practical applications in high precision phase measurement.