Induction of interleukin-1 and subsequent tissue factor expression by anti-proteinase 3 antibodies in human umbilical vein endothelial cells

SummaryIncubation of human umbilical vein endothelial cells with one of the following compounds: endotoxin, recombinant interleukin-1β, recombinant tumor necrosis factor α, allogenic lymphocyte subpopulations or phorbol ester resulted in significant induction of tissue factor synthesis. Diacylglycerol had the same effect and also enhanced synergistically the induction caused by endotoxin and interleukin-1β. Two different inhibitors of protein kinase C, H7 and sphingosine, inhibited tissue factor synthesis at concentrations which did not depress protein synthesis in general, suggesting that protein kinase C is involved in the processes leading to tissue factor synthesis. Cells down-regulated for the tissue factor response to TPA responded essentially normally to endotoxin and interleukin-1 with regard to tissue factor synthesis.

Download Full-text

Correlation of leukocyte interleukin-1 production with the stimulation of prostaglandin and tissue factor synthesis by human umbilical vein endothelial cells

Agents and Actions ◽

10.1007/bf02003232 ◽

1990 ◽

Vol 31 (1-2) ◽

pp. 127-134 ◽

Cited By ~ 8

Author(s):

R. G. Schaub ◽

C. J. Dunn ◽

M. R. Deibel ◽

A. E. Berger ◽

D. Wunderlich ◽

...

Keyword(s):

Endothelial Cells ◽

Tissue Factor ◽

Umbilical Vein ◽

Interleukin 1 ◽

Human Umbilical Vein ◽

Umbilical Vein Endothelial Cells ◽

Stimulation Of

Download Full-text

Studies of the Factor Xa-Dependent Inhibitor of Factor VIIa/Tissue Factor (Extrinsic Pathway Inhibitor) from Cell Supernates of Cultured Human Umbilical Vein Endothelial Cells

Thrombosis and Haemostasis ◽

10.1055/s-0038-1646535 ◽

1989 ◽

Vol 61 (01) ◽

pp. 101-105 ◽

Cited By ~ 27

Author(s):

Bonnie J Warn-Cramer ◽

Fanny E Almus ◽

Samuel I Rapaport

Keyword(s):

Molecular Weight ◽

Endothelial Cells ◽

Tissue Factor ◽

Phorbol Ester ◽

Umbilical Vein ◽

Primary Cultures ◽

Factor Xa ◽

Extrinsic Pathway ◽

Human Umbilical Vein ◽

Umbilical Vein Endothelial Cells

SummaryCultured human umbilical vein endothelial cells (HUVEC) have been reported to produce extrinsic pathway inhibitor (EPI), the factor Xa-dependent inhibitor of factor VHa/tissue factor (TF). We examined the release of this inhibitor from HUVEC as a function of their growth state and in response to the induction of endothelial cell TF activity. HUVEC constitutively produced significant amounts of EPI at all stages of their growth in culture including the post-confluent state. Rate of release varied over a 3-fold range for primary cultures from 12 different batches of pooled umbilical cord cells. Constitutive EPI release was unaltered during a 6 hour period of induction of TF activity with thrombin or phorbol ester but slowed during longer incubation of the cells with phorbol ester. Whereas plasma contains two molecular weight forms of EPI, only the higher of these two molecular weight forms was demonstrable by Western analysis of HUVEC supernatants with 125I-factor Xa as the ligand.

Download Full-text