Sensitive Detection of Small Molecule–Protein Interactions on a Metal–Insulator–Metal Label‐Free Biosensing Platform

2012 ◽  
Vol 7 (8) ◽  
pp. 1867-1874 ◽  
Author(s):  
Amir Syahir ◽  
Kotaro Kajikawa ◽  
Hisakazu Mihara
Keyword(s):  
Small Molecule ◽  
Protein Interactions ◽  
Sensitive Detection ◽  
Label Free ◽  
Metal Insulator ◽  
Metal Insulator Metal

scholarly journals Label-free detection of small-molecule-protein interactions by using nanowire nanosensors

2005 ◽  
Vol 102 (9) ◽  
pp. 3208-3212 ◽  
Author(s):  
W. U. Wang ◽  
C. Chen ◽  
K.-h. Lin ◽  
Y. Fang ◽  
C. M. Lieber
Keyword(s):  
Small Molecule ◽  
Protein Interactions ◽  
Label Free ◽  
Label Free Detection

A New Optical Label-Free Biosensing Platform Based on a Metal−Insulator−Metal Structure

Langmuir ◽  
2010 ◽  
Vol 26 (8) ◽  
pp. 6053-6057 ◽  
Author(s):  
Amir Syahir ◽  
Hisakazu Mihara ◽  
Kotaro Kajikawa
Keyword(s):  
Metal Structure ◽  
Label Free ◽  
Metal Insulator ◽  
Metal Insulator Metal

A simple and sensitive detection of small molecule–protein interactions based on terminal protection-mediated exponential strand displacement amplification

The Analyst ◽  
2018 ◽  
Vol 143 (9) ◽  
pp. 2023-2028 ◽  
Author(s):  
Chang Yeol Lee ◽  
Hyo Yong Kim ◽  
Soeun Kim ◽  
Ki Soo Park ◽  
Hyun Gyu Park
Keyword(s):  
Small Molecule ◽  
Protein Interaction ◽  
Protein Interactions ◽  
Sensitive Detection ◽  
Strand Displacement ◽  
Strand Displacement Amplification ◽  
New Strategy

A new strategy to detect a small molecule–protein interaction was devised based on terminal protection-mediated exponential strand displacement amplification (eSDA).

Terminal protection of small molecule-linked ssDNA for label-free and highly sensitive colorimetric detection of folate receptor biomarkers

RSC Advances ◽  
2015 ◽  
Vol 5 (8) ◽  
pp. 6100-6105 ◽  
Author(s):  
Xue Gong ◽  
Wenjiao Zhou ◽  
Yaqin Chai ◽  
Yun Xiang ◽  
Ruo Yuan
Keyword(s):  
Small Molecule ◽  
Folate Receptor ◽  
Colorimetric Detection ◽  
Sensitive Detection ◽  
Label Free ◽  
Folate Receptors ◽  
Color Transition ◽  
Highly Sensitive

Protection of ssDNA from digesting by Exo I generates amplified color transition for label-free and sensitive detection of folate receptors.

Homogeneous label-free fluorescent assay of small molecule-protein interactions using protein binding-inhibited transcription nanomachine

2011 ◽  
Vol 54 (8) ◽  
pp. 1277-1283 ◽  
Author(s):  
DianMing Zhou ◽  
YiDan Wu ◽  
Pei Liu ◽  
HaoTian Bai ◽  
LiJuan Tang ◽  
...  
Keyword(s):  
Protein Binding ◽  
Small Molecule ◽  
Protein Interactions ◽  
Label Free ◽  
Fluorescent Assay

Plasmonic Metal–Insulator–Metal Capped Polymer Nanopillars for SERS Analysis of Protein–Protein Interactions

2018 ◽  
Vol 122 (11) ◽  
pp. 6255-6266 ◽  
Author(s):  
Lisa P. Hackett ◽  
Wenyue Li ◽  
Abid Ameen ◽  
Lynford L. Goddard ◽  
Gang Logan Liu
Keyword(s):  
Protein Interactions ◽  
Protein Protein Interactions ◽  
Metal Insulator ◽  
Metal Insulator Metal

scholarly journals Progress in the Discovery of Small-Molecule Inhibitors of Bromodomain–Histone Interactions

2011 ◽  
Vol 16 (10) ◽  
pp. 1170-1185 ◽  
Author(s):  
Chun-wa Chung ◽  
Jason Witherington
Keyword(s):  
Small Molecule ◽  
Protein Interactions ◽  
Transcriptional Activation ◽  
Label Free ◽  
Protein Protein Interactions ◽  
Ligand Selectivity ◽  
Small Molecule Ligands ◽  
Associated Proteins ◽  
In Vitro Systems

Bromodomains are structurally conserved protein modules present in a large number of chromatin-associated proteins and in many nuclear histone acetyltransferases. The bromodomain functions as an acetyl-lysine binding domain and has been shown to be pivotal in regulating protein–protein interactions in chromatin-mediated cellular gene transcription, cell proliferation, and viral transcriptional activation. Structural analyses of these modules in complex with acetyl-lysine peptide ligands provide insights into the molecular basis for recognition and ligand selectivity within this epigenetic reader family. However, there are significant challenges in configuring assays to identify inhibitors of these proteins. This review focuses on the progress made in developing methods to identify peptidic and small-molecule ligands using biophysical label-free and biochemical approaches. The advantage of each technique and the results reported are summarized, highlighting the potential applicably to other reader domains and the caveats in translation from simple in vitro systems to a biological context.

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