Immobilization‐Free Electrochemical DNA Sensor based on signal cascade amplification strategy

2021 ◽  
Author(s):  
Guobing Wei ◽  
Wenxing Zhang ◽  
Hanfeng Cui ◽  
Fusheng Liao ◽  
Lin Cheng ◽  
...  
Keyword(s):  
Dna Sensor ◽  
Electrochemical Dna Sensor ◽  
Signal Cascade ◽  
Amplification Strategy ◽  
Cascade Amplification

A signal cascade amplification strategy based on RT-PCR triggering of a G-quadruplex DNAzyme for a novel electrochemical detection of viable Cronobacter sakazakii

The Analyst ◽  
2020 ◽  
Vol 145 (13) ◽  
pp. 4477-4483
Author(s):  
Yuanyuan Yuan ◽  
Xianyong Wu ◽  
Zhanmin Liu ◽  
Qiqi Ning ◽  
Liqiang Fu ◽  
...  
Keyword(s):  
Electrochemical Detection ◽  
Rapid Detection ◽  
Detection Method ◽  
Cronobacter Sakazakii ◽  
Rt Pcr ◽  
Signal Cascade ◽  
G Quadruplex ◽  
Amplification Strategy ◽  
Cascade Amplification

An effective and sensitive DNAzyme method for electrochemical detection of viable Cronobacter sakazakii was designed. The detection method is based on RT-PCR and cascade amplification of ribozymes to achieve rapid detection of viable Cronobacter sakazakii.

Silver Nanoparticles Decorated Polyaniline Nanowires-Based Electrochemical DNA Sensor: Two-step Electrochemical Synthesis

2020 ◽  
Vol 167 (8) ◽  
pp. 087508 ◽  
Author(s):  
Luyen Thi Tran ◽  
Hoang Vinh Tran ◽  
Hue Thi Minh Dang ◽  
Chinh Dang Huynh ◽  
Tuan Anh Mai
Keyword(s):  
Silver Nanoparticles ◽  
Electrochemical Synthesis ◽  
Dna Sensor ◽  
Electrochemical Dna Sensor ◽  
Polyaniline Nanowires

scholarly journals Electrochemical DNA Sensor Based on Carbon Black—Poly(Neutral Red) Composite for Detection of Oxidative DNA Damage

Sensors ◽  
2018 ◽  
Vol 18 (10) ◽  
pp. 3489 ◽  
Author(s):  
Yurii Kuzin ◽  
Dominika Kappo ◽  
Anna Porfireva ◽  
Dmitry Shurpik ◽  
Ivan Stoikov ◽  
...  
Keyword(s):  
Carbon Black ◽  
Oxidative Dna Damage ◽  
Electrochemical Impedance ◽  
Neutral Red ◽  
Redox Activity ◽  
Antitumor Drugs ◽  
Oxygen Species ◽  
Dna Sensor ◽  
Electrochemical Dna Sensor ◽  
Polymeric Dye

Voltammetric DNA sensor has been proposed on the platform of glassy carbon electrode covered with carbon black with adsorbed pillar[5]arene molecules. Electropolymerization of Neutral Red performed in the presence of native or oxidatively damaged DNA resulted in formation of hybrid material which activity depended on the DNA conditions. The assembling of the surface layer was confirmed by scanning electron microscopy and electrochemical impedance spectroscopy. The influence of DNA and pillar[5]arene on redox activity of polymeric dye was investigated and a significant increase of the peak currents was found for DNA damaged by reactive oxygen species generated by Cu2+/H2O2 mixture. Pillar[5]arene improves the electron exchange conditions and increases the response and its reproducibility. The applicability of the DNA sensor developed was shown on the example of ascorbic acid as antioxidant. It decreases the current in the concentration range from 1.0 μM to 1.0 mM. The possibility to detect antioxidant activity was qualitatively confirmed by testing tera infusion. The DNA sensor developed can find application in testing of carcinogenic species and searching for new antitumor drugs.

scholarly journals Sensitive electrochemical DNA sensor for the detection of HIV based on a polyaniline/graphene nanocomposite

2019 ◽  
Vol 5 (2) ◽  
pp. 313-319 ◽  
Author(s):  
Qiaojuan Gong ◽  
Haixia Han ◽  
Haiying Yang ◽  
Meiling Zhang ◽  
Xiaoling Sun ◽  
...  
Keyword(s):  
Dna Sensor ◽  
Electrochemical Dna Sensor ◽  
Graphene Nanocomposite

ELECTROCHEMICAL DNA SENSOR TECHNOLOGY FOR MONITORING OF DRUG–DNA INTERACTIONS

NANO ◽  
2008 ◽  
Vol 03 (04) ◽  
pp. 229-232 ◽  
Author(s):  
A. ERDEM ◽  
H. KARADENIZ ◽  
A. CALISKAN ◽  
A. VASEASHTA
Keyword(s):  
Drug Delivery ◽  
Small Molecules ◽  
Anticancer Drugs ◽  
Point Of Care ◽  
Sensor Technology ◽  
Electrochemical Biosensors ◽  
Dna Sensor ◽  
Dna Interactions ◽  
Electrochemical Dna Sensor

The objective of this investigation is to understand the nature and dynamics of binding small molecules to bio-macromolecules using electrochemical methods. The investigation pertaining to the design of site- and conformation-specific reagents provides a rationale for new studies of drug delivery design. Some anticancer drugs and DNA interactions have been undertaken by using a variety of techniques. Determination of interaction between DNA and DNA-targeted molecules would be valuable in the design of molecule-specific electrochemical biosensors for applications in diagnostics, development of drugs for chemotherapy, and as a biotechnological tool for DNA-based point-of-care diagnosis.

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