Characterization of a putative tropinone reductase from Tarenaya hassleriana with a broad substrate specificity

2021 ◽  
Author(s):  
Yixiang Li ◽  
Yajun Bai ◽  
Tai‐Ping Fan ◽  
Xiaohui Zheng ◽  
Yujie Cai
Keyword(s):  
Substrate Specificity ◽  
Broad Substrate Specificity ◽  
Tropinone Reductase

scholarly journals Functional and structural characterization of IdnL7, an adenylation enzyme involved in incednine biosynthesis

2019 ◽  
Vol 75 (4) ◽  
pp. 299-306
Author(s):  
Jolanta Cieślak ◽  
Akimasa Miyanaga ◽  
Makoto Takaishi ◽  
Fumitaka Kudo ◽  
Tadashi Eguchi
Keyword(s):  
Amino Acids ◽  
Substrate Specificity ◽  
Natural Product ◽  
Structural Characterization ◽  
Biochemical Analysis ◽  
Natural Product Biosynthesis ◽  
Broad Substrate Specificity ◽  
Selective Incorporation ◽  
Polyketide Antibiotic

Adenylation enzymes play an important role in the selective incorporation of the cognate carboxylate substrates in natural product biosynthesis. Here, the biochemical and structural characterization of the adenylation enzyme IdnL7, which is involved in the biosynthesis of the macrolactam polyketide antibiotic incednine, is reported. Biochemical analysis showed that IdnL7 selects and activates several small amino acids. The structure of IdnL7 in complex with an L-alanyl-adenylate intermediate mimic, 5′-O-[N-(L-alanyl)sulfamoyl]adenosine, was determined at 2.1 Å resolution. The structure of IdnL7 explains the broad substrate specificity of IdnL7 towards small L-amino acids.

scholarly journals Erratum to: Characterization of a GH family 8 β-1,3-1,4-glucanase with distinctive broad substrate specificity from Paenibacillus sp. X4

2014 ◽  
Vol 37 (3) ◽  
pp. 657-658
Author(s):  
Han Beur Na ◽  
Won Kyeong Jung ◽  
Yu Seok Jeong ◽  
Hee Jung Kim ◽  
Sung Kyum Kim ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Broad Substrate Specificity ◽  
Paenibacillus Sp

scholarly journals Characterization of GDP-mannose pyrophosphorylase from Escherichia coli O157:H7 EDL933 and its broad substrate specificity

2005 ◽  
Vol 37 (1-6) ◽  
pp. 1-8 ◽  
Author(s):  
Yung-Hun Yang ◽  
Young-Bok Kang ◽  
Kwang-Won Lee ◽  
Tek-Hyung Lee ◽  
Sung-Soo Park ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Substrate Specificity ◽  
Escherichia Coli O157 ◽  
Broad Substrate Specificity
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