Screening of ten cytochrome P450 enzyme activities with 12 probe substrates in human liver microsomes using cocktail incubation and liquid chromatography–tandem mass spectrometry

2019 ◽  
Vol 40 (3-4) ◽  
pp. 101-111 ◽  
Author(s):  
Hyun‐Ji Kim ◽  
Hyunyoung Lee ◽  
Hyeon‐Kyeong Ji ◽  
Taeho Lee ◽  
Kwang‐Hyeon Liu
Keyword(s):  
Mass Spectrometry ◽  
Cytochrome P450 ◽  
Liquid Chromatography ◽  
Human Liver ◽  
Liver Microsomes ◽  
Cytochrome P450 Enzyme ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
P450 Enzyme ◽  
Liquid Chromatography Tandem Mass

scholarly journals Reactive intermediates in naquotinib metabolism identified by liquid chromatography-tandem mass spectrometry: phase I metabolic profiling

RSC Advances ◽  
2019 ◽  
Vol 9 (18) ◽  
pp. 10211-10225 ◽  
Author(s):  
Mohamed W. Attwa ◽  
Adnan A. Kadi ◽  
Haitham AlRabiah ◽  
Hany W. Darwish
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Metabolic Profiling ◽  
Human Liver ◽  
Liver Microsomes ◽  
Reactive Intermediates ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

LC-MS/MS was used to screen for in vitro metabolites of NQT formed during incubation with human liver microsomes (HLMs) and then evaluated the generation of reactive electrophiles using capturing agents.

Sign in / Sign up

Export Citation Format

Share Document