D-Mannitol production by resting state whole cell biotransformation ofD-fructose by heterologous mannitol and formate dehydrogenase gene expression inBacillus megaterium

2007 ◽  
Vol 2 (11) ◽  
pp. 1408-1416 ◽  
Author(s):  
Carsten Bäumchen ◽  
Andreas H. F. J. Roth ◽  
Rebekka Biedendieck ◽  
Marco Malten ◽  
Martin Follmann ◽  
...  
Keyword(s):  
Gene Expression ◽  
Resting State ◽  
Formate Dehydrogenase ◽  
Whole Cell ◽  
Dehydrogenase Gene ◽  
Mannitol Production ◽  
Whole Cell Biotransformation

scholarly journals Function and Regulation of the Formate Dehydrogenase Genes of the Methanogenic Archaeon Methanococcus maripaludis

2003 ◽  
Vol 185 (8) ◽  
pp. 2548-2554 ◽  
Author(s):  
Gwendolyn E. Wood ◽  
Andrew K. Haydock ◽  
John A. Leigh
Keyword(s):  
Gene Expression ◽  
Gene Cluster ◽  
Double Mutant ◽  
Formate Dehydrogenase ◽  
Gene Clusters ◽  
Growth Defect ◽  
Methanococcus Maripaludis ◽  
Gene Sets ◽  
Dehydrogenase Gene ◽  
Duplication Events

ABSTRACT Methanococcus maripaludis is a mesophilic species of Archaea capable of producing methane from two substrates: hydrogen plus carbon dioxide and formate. To study the latter, we identified the formate dehydrogenase genes of M. maripaludis and found that the genome contains two gene clusters important for formate utilization. Phylogenetic analysis suggested that the two formate dehydrogenase gene sets arose from duplication events within the methanococcal lineage. The first gene cluster encodes homologs of formate dehydrogenase α (FdhA) and β (FdhB) subunits and a putative formate transporter (FdhC) as well as a carbonic anhydrase analog. The second gene cluster encodes only FdhA and FdhB homologs. Mutants lacking either fdhA gene exhibited a partial growth defect on formate, whereas a double mutant was completely unable to grow on formate as a sole methanogenic substrate. Investigation of fdh gene expression revealed that transcription of both gene clusters is controlled by the presence of H2 and not by the presence of formate.

Expression of the NADP+-Dependent Formate Dehydrogenase Gene from Pseudomonas Increases the Lysine Production in Corynebacterium glutamicum

2019 ◽  
Vol 35 (6) ◽  
pp. 21-29
Author(s):  
T.E. Leonova ◽  
T.E. Shustikova ◽  
T.V. Gerasimova ◽  
Т.А. Ivankova ◽  
K.V. Sidorenko Sidorenko ◽  
...  
Keyword(s):  
Corynebacterium Glutamicum ◽  
Formate Dehydrogenase ◽  
Ministry Of Education ◽  
Lysine Production ◽  
Simultaneous Formation ◽  
Resource Center ◽  
Formate Oxidation ◽  
Gene Coding ◽  
Dehydrogenase Gene ◽  
Lysine Synthesis

Thepsefdh_D221Q gene coding for a mutant formate dehydrogenase (PseFDG_D221Q) from Pseudomonas, which catalyzes the formate oxidation with the simultaneous formation of NADPH, has been expressed in the cells of lysine-producing Corynebacterium glutamicum strains. The psefdh_D221Q gene was introduced into С. glutamicum strains as part of an autonomous plasmid or was integrated into the chromosome with simultaneous inactivation of host formate dehydrogenase genes. It was shown that the С. glutamicum strains with NADP+ -dependent formate dehydrogenase have an increased level of L-lysine synthesis in the presence of formate, if their own formate dehydrogenase is inactivated. L-lysine, formate dehydrogenase, NADPH, Corynebacterium glutamicum The work was carried out using the equipment of the Multipurpose Scientific This work was carried out on the equipment of the Multipurpose Scientific Installation of «All-Russian Collection of Industrial Microorganisms», National Bio-Resource Center, NRC «Kurchatov Institute»- GosNIIgenetika. This work was financially supported by the Ministry of Education and Science of Russia (Unique Project Identifier - RFMEFI61017X0011).

Asymmetric reduction of a key intermediate of eslicarbazepine acetate using whole cell biotransformation in a biphasic medium

2012 ◽  
Vol 2 (8) ◽  
pp. 1602 ◽  
Author(s):  
Manpreet Singh ◽  
Sawraj Singh ◽  
Sateesh Deshaboina ◽  
Hare Krishnen ◽  
Richard Lloyd ◽  
...  
Keyword(s):  
Asymmetric Reduction ◽  
Eslicarbazepine Acetate ◽  
Whole Cell ◽  
Whole Cell Biotransformation ◽  
Biphasic Medium

Glyoxylate Carboligase-based Whole-Cell Biotransformation of Formaldehyde into Ethylene Glycol via Glycolaldehyde

2021 ◽  
Author(s):  
Hye-Jin Jo ◽  
Jun-Hong Kim ◽  
Ye-Na Kim ◽  
Pil-Won Seo ◽  
Chae-Yun Kim ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Whole Cell ◽  
Whole Cell Biotransformation

A novel biocatalytic system for the synthesis of the industrially relevant C2 chemicals (e.g., ethylene glycol (3)) from formaldehyde (1) was established. The biocatalytic system consisted of a newly discovered...

Glucocorticoid upregulation of glutamate dehydrogenase gene expression in vitro in astrocytes

1996 ◽  
Vol 37 (1-2) ◽  
pp. 324-328 ◽  
Author(s):  
H. Hardin-Pouzet ◽  
P. Giraudon ◽  
M.F. Belin ◽  
M. Didier-Bazes
Keyword(s):  
Gene Expression ◽  
Glutamate Dehydrogenase ◽  
Dehydrogenase Gene

New fungi for whole-cell biotransformation of carvone enantiomers. Novel p-menthane-2,8,9-triols production

2013 ◽  
Vol 468 ◽  
pp. 88-94 ◽  
Author(s):  
Fátima M. Nunes ◽  
Gabriel F. dos Santos ◽  
Natália N. Saraiva ◽  
Marília A. Trapp ◽  
Marcos C. de Mattos ◽  
...  
Keyword(s):  
Whole Cell ◽  
Whole Cell Biotransformation
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