CRISPR/Cas9 mediated targeted mutagenesis of Liguleless1 in sorghum provides a rapidly scorable phenotype by altering leaf inclination angle

2021 ◽  
pp. 2100237
Author(s):  
Eleanor J Brant ◽  
Mehmet Cengiz Baloglu ◽  
Aalap Parikh ◽  
Fredy Altpeter
2021 ◽  
Author(s):  
xiuqing fu ◽  
Jianbo He ◽  
Zhuangzhuang Sun ◽  
Hongwen Zhang ◽  
Jieyu Xian

Abstract Background:To investigate the effect of drought stress on wheat posture.Methods:An image acquisition system based on an infrared tracing robot was developed and a graphical user interface (GUI) software was designed to simplify the operation control of the robot. In this experiment, three genotypes of wheat, Ruihuamai 523, Jimai 22 and Xumai 33, were grown in indoor pots, and the images of wheatposture from flowering stage to maturity were collected to extract morphological parameters such as plant height, stem width and leaf inclination angle. Results:The experimental results showed that the deviation of linear trajectory was less than 3 mm when the robot traveled in a straight line at 0.4 m/s in the greenhouse; the image acquisition efficiency was about 18 images/min;the collected pictures of drought-stressed and control potted wheat groups can be used for posture assessment; the accuracy of wheat plant height and stem width with manual acquisition was 84.6% and 79.2%, respectively. After statistical analysis, it was concluded that drought stress and genotype and other influencing factors had no significant effect on plant height and stem width, but had a greater effect on leaf inclination angle.Conclusions:Therefore, this system can be used for posture collection of wheat.


2021 ◽  
Vol 2021 ◽  
pp. 1-15
Author(s):  
Guangjian Yan ◽  
Hailan Jiang ◽  
Jinghui Luo ◽  
Xihan Mu ◽  
Fan Li ◽  
...  

Both leaf inclination angle distribution (LAD) and leaf area index (LAI) dominate optical remote sensing signals. The G-function, which is a function of LAD and remote sensing geometry, is often set to 0.5 in the LAI retrieval of coniferous canopies even though this assumption is only valid for spherical LAD. Large uncertainties are thus introduced. However, because numerous tiny leaves grow on conifers, it is nearly impossible to quantitatively evaluate such uncertainties in LAI retrieval. In this study, we proposed a method to characterize the possible change of G-function of coniferous canopies as well as its effect on LAI retrieval. Specifically, a Multi-Directional Imager (MDI) was developed to capture stereo images of the branches, and the needles were reconstructed. The accuracy of the inclination angles calculated from the reconstructed needles was high. Moreover, we analyzed whether a spherical distribution is a valid assumption for coniferous canopies by calculating the possible range of the G-function from the measured LADs of branches of Larch and Spruce and the true G-functions of other species from some existing inventory data and three-dimensional (3D) tree models. Results show that the constant G assumption introduces large errors in LAI retrieval, which could be as large as 53% in the zenithal viewing direction used by spaceborne LiDAR. As a result, accurate LAD estimation is recommended. In the absence of such data, our results show that a viewing zenith angle between 45 and 65 degrees is a good choice, at which the errors of LAI retrieval caused by the spherical assumption will be less than 10% for coniferous canopies.


2018 ◽  
Vol 21 (4) ◽  
pp. 302-310 ◽  
Author(s):  
Nan Su San ◽  
Masahiro Yamashita ◽  
Shunsuke Adachi ◽  
Takanari Tanabata ◽  
Taiichiro Ookawa ◽  
...  

Sensors ◽  
2018 ◽  
Vol 18 (10) ◽  
pp. 3576 ◽  
Author(s):  
Kenta Itakura ◽  
Fumiki Hosoi

Automatic and efficient plant monitoring offers accurate plant management. Construction of three-dimensional (3D) models of plants and acquisition of their spatial information is an effective method for obtaining plant structural parameters. Here, 3D images of leaves constructed with multiple scenes taken from different positions were segmented automatically for the automatic retrieval of leaf areas and inclination angles. First, for the initial segmentation, leave images were viewed from the top, then leaves in the top-view images were segmented using distance transform and the watershed algorithm. Next, the images of leaves after the initial segmentation were reduced by 90%, and the seed regions for each leaf were produced. The seed region was re-projected onto the 3D images, and each leaf was segmented by expanding the seed region with the 3D information. After leaf segmentation, the leaf area of each leaf and its inclination angle were estimated accurately via a voxel-based calculation. As a result, leaf area and leaf inclination angle were estimated accurately after automatic leaf segmentation. This method for automatic plant structure analysis allows accurate and efficient plant breeding and growth management.


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