scholarly journals Recent developments in the site-specific immobilization of proteins onto solid supports

Biopolymers ◽  
2007 ◽  
Vol 90 (3) ◽  
pp. 450-458 ◽  
Author(s):  
Julio A. Camarero
Keyword(s):  
Solid Supports ◽  
Site Specific ◽  
Recent Developments

Comparison of site-specific coupling chemistry for antibody immobilization on different solid supports

1991 ◽  
Vol 542 ◽  
pp. 41-54 ◽  
Author(s):  
Lin Jinn-Nan ◽  
Chang I-Nan ◽  
Joseph D. Andrade ◽  
James N. Herron ◽  
Douglas A. Christensen
Keyword(s):  
Antibody Immobilization ◽  
Solid Supports ◽  
Site Specific

Site-specific PEGylation of Proteins: Recent Developments

2014 ◽  
Vol 79 (22) ◽  
pp. 10727-10733 ◽  
Author(s):  
Nicole Nischan ◽  
Christian P. R. Hackenberger
Keyword(s):  
Site Specific ◽  
Recent Developments

scholarly journals Organometallic chemical biology: an organometallic approach to bioconjugation

2017 ◽  
Vol 89 (11) ◽  
pp. 1619-1640 ◽  
Author(s):  
Ekaterina V. Vinogradova
Keyword(s):  
Organometallic Chemistry ◽  
Chemical Biology ◽  
Structure And Function ◽  
Future Prospects ◽  
Site Specific ◽  
Organometallic Reagents ◽  
Bond Forming ◽  
Spatially Resolved ◽  
Recent Developments ◽  
And Function

AbstractThis review summarizes the history and recent developments of the field of organometallic chemical biology with a particular emphasis on the development of novel bioconjugation approaches. Over the years, numerous transformations have emerged for biomolecule modification with the use of organometallic reagents; these include [3+2] cycloadditions, C–C, C–S, C–N, and C–O bond forming processes, as well as metal-mediated deprotection (“decaging”) reactions. These conceptually new additions to the chemical biology toolkit highlight the potential of organometallic chemistry to make a significant impact in the field of chemical biology by providing further opportunities for the development of chemoselective, site-specific and spatially resolved methods for biomolecule structure and function manipulation. Examples of these transformations, as well as existing challenges and future prospects of this rapidly developing field are highlighted in this review.

scholarly journals Challenging a Paradigm: the Role of DNA Homology in Tyrosine Recombinase Reactions

2009 ◽  
Vol 73 (2) ◽  
pp. 300-309 ◽  
Author(s):  
Lara Rajeev ◽  
Karolina Malanowska ◽  
Jeffrey F. Gardner
Keyword(s):  
Dna Homology ◽  
Tyrosine Recombinase ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Conjugative Transposons ◽  
The Core ◽  
Sequence Identity ◽  
Tyrosine Recombinases ◽  
Recent Developments

SUMMARY A classical feature of the tyrosine recombinase family of proteins catalyzing site-specific recombination, as exemplified by the phage lambda integrase and the Cre and Flp recombinases, is the ability to recombine substrates sharing very limited DNA sequence identity. Decades of research have established the importance of this short stretch of identity within the core regions of the substrates. Since then, several new enzymes that challenge this paradigm have been discovered and require the role of sequence identity in site-specific recombination to be reconsidered. The integrases of the conjugative transposons such as Tn916, Tn1545, and CTnDOT recombine substrates with heterologous core sequences. The integrase of the mobilizable transposon NBU1 performs recombination more efficiently with certain core mismatches. The integration of CTX phage and capture of gene cassettes by integrons also occur by altered mechanisms. In these systems, recombination occurs between mismatched sequences by a single strand exchange. In this review, we discuss literature that led to the formulation of the current strand-swapping isomerization model for tyrosine recombinases. The review then focuses on recent developments on the recombinases that challenged the paradigm that was derived from the studies of early systems.

scholarly journals Recent Developments in CrossBeam® Technology

2007 ◽  
Vol 15 (1) ◽  
pp. 18-19
Author(s):  
A. Thesen ◽  
H. Hoffmeister ◽  
M. Schumann ◽  
P. Gnauck
Keyword(s):  
High Resolution ◽  
Cross Sections ◽  
Focused Ion Beam ◽  
Materials Science ◽  
Ion Beam ◽  
Deposition Process ◽  
Injection System ◽  
Site Specific ◽  
Submicron Scale ◽  
Recent Developments

Recent developments in nano- and semiconductor technology have substantially increased the demand for accurate and efficient site specific cross-sectioning of specimens and preparation of TEM samples. Moreover, nano-research is facing new challenges for manipulation, observation, and modification of devices on a submicron scale. At the same time in materials science a new focus on analytical nanoscale investigations—not only of specimen surfaces and cross sections—but on sample volumes is emerging.These demanding requirements can be met if a focused ion beam (FIB) column for nanoscale structuring is combined with a high resolution SEM that is used to monitor the FIB milling and deposition process on a nanometer scale. Such an integrated Cross-Beam® system enables the high resolution observation and direct control of the FIB milling process in real time. Using this concept it is possible to prepare site specific TEM samples and cross sections with nano-scale accuracy. Such a system can be complemented with a gas injection system (GIS), for deposition and enhanced etching of specific materials, as well as, in-situ micro manipulation systems, and analytical detectors such as EDX and EBSP systems.

scholarly journals Supported Palladium Nanocatalysts: Recent Findings in Hydrogenation Reactions

Processes ◽  
2020 ◽  
Vol 8 (9) ◽  
pp. 1172
Author(s):  
Marta A. Andrade ◽  
Luísa M. D. R. S. Martins
Keyword(s):  
Metallic Nanoparticles ◽  
Pd Nanoparticles ◽  
Solid Supports ◽  
Research Areas ◽  
Wide Range ◽  
Palladium Nanocatalysts ◽  
Recent Developments ◽  
Hydrogenation Reactions ◽  
Metal Organic ◽  
Major Factors

Catalysis has witnessed a dramatic increase on the use of metallic nanoparticles in the last decade, opening endless opportunities in a wide range of research areas. As one of the most investigated catalysts in organic synthesis, palladium finds numerous applications being of significant relevance in industrial hydrogenation reactions. The immobilization of Pd nanoparticles in porous solid supports offers great advantages in heterogeneous catalysis, allowing control of the major factors that influence activity and selectivity. The present review deals with recent developments in the preparation and applications of immobilized Pd nanoparticles on solid supports as catalysts for hydrogenation reactions, aiming to give an insight on the key factors that contribute to enhanced activity and selectivity. The application of mesoporous silicas, carbonaceous materials, zeolites, and metal organic frameworks (MOFs) as supports for palladium nanoparticles is addressed.

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