scholarly journals Contribution of gene expression to metabolic fluxes in hypermetabolic livers induced through burn injury and cecal ligation and puncture in rats

2007 ◽  
Vol 97 (1) ◽  
pp. 118-137 ◽  
Author(s):  
Scott Banta ◽  
Murali Vemula ◽  
Tadaaki Yokoyama ◽  
Arul Jayaraman ◽  
François Berthiaume ◽  
...  
Keyword(s):  
Gene Expression ◽  
Burn Injury ◽  
Cecal Ligation ◽  
Cecal Ligation And Puncture ◽  
Metabolic Fluxes

Intrahepatic STAT-3 activation and acute phase gene expression predict outcome after CLP sepsis in the rat

1998 ◽  
Vol 275 (6) ◽  
pp. G1423-G1429 ◽  
Author(s):  
Kenneth M. Andrejko ◽  
Jodi Chen ◽  
Clifford S. Deutschman
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Acute Phase ◽  
Interleukin 6 ◽  
Cecal Ligation ◽  
Cecal Ligation And Puncture ◽  
Signal Transducer ◽  
Lethal Sepsis

Interleukin-6 (IL-6) regulates hepatic acute phase responses by activating the transcription factor signal transducer and activator of transcription (STAT)-3. IL-6 also may modulate septic pathophysiology. We hypothesize that 1) STAT-3 activation and transcription of α2-macroglobulin (A2M) correlate with recovery from sepsis and 2) STAT-3 activation and A2M transcription reflect intrahepatic and not serum IL-6. Nonlethal sepsis was induced in rats by single puncture cecal ligation and puncture (CLP) and lethal sepsis via double-puncture CLP. STAT-3 activation and A2M transcription were detected at 3–72 h and intrahepatic IL-6 at 24–72 h following single-puncture CLP. All were detected only at 3–16 h following double-puncture CLP and at lower levels than following single-puncture CLP. Loss of serum and intrahepatic IL-6 activity after double-puncture CLP correlated with mortality. Neither intrahepatic nor serum IL-6 levels correlated with intrahepatic IL-6 activity. STAT-3 activation following single-puncture CLP inversely correlated with altered transcription of gluconeogenic, ketogenic, and ureagenic genes. IL-6 may have both beneficial and detrimental effects in sepsis. Fulminant sepsis may decrease the ability of hepatocytes to respond to IL-6.

SEPSIS GENE EXPRESSION PROFILING II: EFFECT OF ANTIBIOTICS OVER TIME ON MURINE SPLENOCYTES AFTER CECAL LIGATION AND PUNCTURE.

Shock ◽  
2001 ◽  
Vol 15 (Supplement) ◽  
pp. 26 ◽  
Author(s):  
T P Chung ◽  
R S Hotchkiss ◽  
J M Laramie ◽  
Y Qiu ◽  
M A Watson ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Cecal Ligation ◽  
Cecal Ligation And Puncture ◽  
Murine Splenocytes ◽  
Over Time

scholarly journals Dynamics of Hepatic Gene Expression Profile in a Rat Cecal Ligation and Puncture Model

2012 ◽  
Vol 176 (2) ◽  
pp. 583-600 ◽  
Author(s):  
Qian Yang ◽  
John S.A. Mattick ◽  
Mehmet A. Orman ◽  
Tung T. Nguyen ◽  
Marianthi G. Ierapetritou ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Cecal Ligation ◽  
Hepatic Gene Expression ◽  
Cecal Ligation And Puncture ◽  
Puncture Model

scholarly journals Differences in Inflammation, Apoptosis and Tissue Hypo-perfusion Indicators Caused by Using Two Different Gauges in Cecal Ligation and Puncture-induced Rat Models of Sepsis

2020 ◽  
Author(s):  
Tina Didari ◽  
Maryam Baeeri ◽  
Mahban Rahimifard ◽  
Shilan Mozaffari ◽  
Hamed Haghi-Aminjan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Low Cost ◽  
Cecal Ligation ◽  
Organ Damage ◽  
Cecal Ligation And Puncture ◽  
Cytokine Storm ◽  
Blood Profile ◽  
Needle Size ◽  
Gauge Groups ◽  
G 21

Abstract Background: Rodent Cecal Ligation and Puncture (CLP) models, which mimic sepsis in humans. Gauges needle size during CLP related to cytokine storm, inflammation and organ failure.This study focus on precise and low cost various biochemical markers to trace sepsis severity in cardiopulmonary system and blood after CLP with gauge needle size -18 (G-18) and -21 (G-21) in rats.Results: For the purpose of this study, 18 male Wistar rats were assigned into three groups: CLP procedure with G-18 needle, CLP procedure with G-21 and sham. Following the procedure, oxidative stress markers, blood markers, apoptosis indicators, gene expression of autophagy and cellular hypoxia and histopathological assessments were carried out in the cardiopulmonary tissue and blood 24 hours after CLP. All measured factors were increased in both G-18 and G-21 gauge groups in comparison with the sham. Moreover, 24 hours after CLP, inflammatory markers, blood profile, lactate level, pro-inflammatory cytokines, caspases, gene expression of cellular homeostasis and autophagy in samples were more pronouncedly increased in the G-18 group in comparison with the G-21. Conclusions: This experiment provides a significant association of inflammation and cytokine storm with anti-oxidant property, apoptotic condition and gene expression of tissue and blood infection related to severity organ damage were increased with G-18 compare to G-21. Our study has identified cost-effective indicators to evaluate organ failure during sepsis. This should be considered by all investigators using G-18 in comparison with G-21 to induce severe sepsis.

Long-term gene expression profile dynamics following cecal ligation and puncture in the rat

2012 ◽  
Vol 178 (1) ◽  
pp. 431-442 ◽  
Author(s):  
John S.A. Mattick ◽  
Qian Yang ◽  
Mehmet A. Orman ◽  
Marianthi G. Ierapetritou ◽  
Francois Berthiaume ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Cecal Ligation ◽  
Cecal Ligation And Puncture
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