Defining process design space for a hydrophobic interaction chromatography (HIC) purification step: Application of quality by design (QbD) principles

2010 ◽  
Vol 107 (6) ◽  
pp. 985-997 ◽  
Author(s):  
Canping Jiang ◽  
Lisa Flansburg ◽  
Sanchayita Ghose ◽  
Paul Jorjorian ◽  
Abhinav A. Shukla
Keyword(s):  
Hydrophobic Interaction ◽  
Process Design ◽  
Quality By Design ◽  
Design Space ◽  
Hydrophobic Interaction Chromatography ◽  
Purification Step

scholarly journals A Simple Method for the Estimation of Protein Retention in Hydrophobic Interaction Chromatography Under Different Operation Conditions

2007 ◽  
Vol 1 (1) ◽  
pp. 9-13 ◽  
Author(s):  
Andrea Mahn ◽  
M. Elena Lienqueo ◽  
Juan A. Asenjo
Keyword(s):  
Hydrophobic Interaction ◽  
Linear Correlation ◽  
Retention Time ◽  
Process Design ◽  
Scale Up ◽  
Hydrophobic Interaction Chromatography ◽  
Simple Method ◽  
Protein Retention ◽  
Operation Conditions

Protein behavior in Hydrophobic Interaction Chromatography using different chromatographic conditions was investigated. A linear correlation was found between protein retention time on different matrixes and different initial elution salt concentrations. Mathematical correlations between retention times under different chromatographic conditions were obtained and validated, which can be used in process design and scale-up.

Purification of Glycerol Kinase by "Dye-Ligand" Chromatography and Hydrophobic Interaction Chromatography on Bead-Cellulose Derivatives

1993 ◽  
Vol 58 (2) ◽  
pp. 445-451 ◽  
Author(s):  
Vladimír Žúbor ◽  
Albert Breier ◽  
Marta Horváthová ◽  
Dagmar Hagarová ◽  
Peter Gemeiner ◽  
...  
Keyword(s):  
Hydrophobic Interaction ◽  
Specific Activity ◽  
Hydrophobic Interaction Chromatography ◽  
Glycerol Kinase ◽  
Enzymic Activity ◽  
Cellulose Derivatives ◽  
Long Term Storage ◽  
Bead Cellulose ◽  
Term Storage

The crude extract of cytosole enzymes was obtained from homogenized cells of Saccharomyces cerevisiae by partition. The enzyme was then isolated from the lower aqueous phase displaying higher glycerol kinase activity by dye-ligand chromatography on Cibacron Blue (CB) or Remazol Brilliant Blue R (RB)-derivatized bead-cellulose, ATP being the eluent. The specific activity of glycerol kinase rised more than 10 and 7-times after affinity dye-ligand chromatography and hydrophobic interaction chromatography, respectively. Glycerol kinase obtained by the latter method was purified by CB-bead cellulose. The final preparation maintained its enzymic activity without noticeable losses during a long-term storage at 4 °C in dark.

Possibilities and limitations of hydrophobic interaction chromatography evaluation with strepavidin

1986 ◽  
Vol 22 (7-12) ◽  
pp. 391-398 ◽  
Author(s):  
E. Reh ◽  
C. Birkner ◽  
M. P. Weigert
Keyword(s):  
Hydrophobic Interaction ◽  
Hydrophobic Interaction Chromatography
Sign in / Sign up

Export Citation Format

Share Document