Laser exposure of gold nanorods can increase neuronal cell outgrowth

2013 ◽  
Vol 110 (8) ◽  
pp. 2277-2291 ◽  
Author(s):  
Chiara Paviolo ◽  
John W. Haycock ◽  
Jiawey Yong ◽  
Aimin Yu ◽  
Paul R. Stoddart ◽  
...  
Keyword(s):  
Gold Nanorods ◽  
Neuronal Cell ◽  
Laser Exposure ◽  
Cell Outgrowth

scholarly journals Non-neuronal cell outgrowth inC. elegans

Worm ◽  
2017 ◽  
Vol 6 (3-4) ◽  
pp. e1405212 ◽  
Author(s):  
Srimoyee Ghosh ◽  
Sylvia A. Vetrone ◽  
Paul W. Sternberg
Keyword(s):  
Neuronal Cell ◽  
Cell Outgrowth

scholarly journals Doxorubicin loaded gold nanorods: a multifunctional chemo-photothermal nano-platform for cancer management

2020 ◽  
Author(s):  
Uzma Azeem Awan ◽  
Abida Raza ◽  
Shaukat Ali ◽  
Rida Fatima Saeed ◽  
Nosheen Akhtar
Keyword(s):  
Side Effects ◽  
Laser Irradiation ◽  
Gold Nanorods ◽  
Drug Loading ◽  
Maximum Efficiency ◽  
High Dose ◽  
Laser Exposure ◽  
Cancer Management ◽  
Cell Inhibition ◽  
Nir Laser

One of the limitations associated with cancer treatment is low efficacy and high dose-related side effects of anticancer drugs. The purpose of the current study was to fabricate biocompatible multifunctional drug loaded nano-moieties for co-therapy (chemo-photothermal therapy) with maximum efficiency and minimum side effects. Herein, we report in vitro anticancerous effects of doxorubicin (DOX) loaded on polyelectrolyte-poly (sodium-4-styrenesulfonate) coated Gold nanorods (PSS-GNRs) with and without NIR laser (808 nm, power density = 1.5 W/cm2 for 2 min) exposure. Drug loading capacity of PSS-GNRs was about 76% with drug loading content of 3.2 mg DOX/mL. Cumulative DOX release significantly increased after laser exposure (1.5 W/cm2) compared to non-irradiated samples (p<0.05). Zeta potential of GNRs, PSS-GNRs and DOX-PSS-GNRs was recorded as +42 ±0.1 mV, – 40 ±0.3 mV and 39.3 ±0.6 mV, respectively. PSS-GNRs nano-complexes were found biocompatible and showed higher photothermal stability. DOX conjugated nano-complexes with NIR laser irradiation appear more efficient in cell inhibition (93%) than without laser exposure (65%) and doxorubicin alone (84%). The IC50 of PSS-GNRs-DOX and PSS-GNRs-DOX was recorded as 7.99 ±0.0032 and 3.12 ±0.0906 µg/mL with laser irradiation. Thus, a combinatorial approach based on chemo and photothermal strategy appears to be a promising platform in cancer management.

scholarly journals Doxorubicin-loaded gold nanorods: a multifunctional chemo-photothermal nanoplatform for cancer management

2021 ◽  
Vol 12 ◽  
pp. 295-303
Author(s):  
Uzma Azeem Awan ◽  
Abida Raza ◽  
Shaukat Ali ◽  
Rida Fatima Saeed ◽  
Nosheen Akhtar
Keyword(s):  
Side Effects ◽  
Laser Irradiation ◽  
Gold Nanorods ◽  
Drug Loading ◽  
High Dose ◽  
Laser Exposure ◽  
Cancer Management ◽  
Cell Inhibition ◽  
Nir Laser

Two of the limitations associated with cancer treatment are the low efficacy and the high dose-related side effects of anticancer drugs. The purpose of the current study was to fabricate biocompatible multifunctional drug-loaded nanoscale moieties for co-therapy (chemo-photothermal therapy) with maximum efficacy and minimum side effects. Herein, we report in vitro anticancerous effects of doxorubicin (DOX) loaded on gold nanorods coated with the polyelectrolyte poly(sodium-4-styrenesulfonate) (PSS-GNRs) with and without NIR laser (808 nm, power density = 1.5 W/cm2 for 2 min) irradiation. The drug-loading capacity of PSS-GNRs was about 76% with a drug loading content of 3.2 mg DOX/mL. The cumulative DOX release significantly increased after laser exposure compared to non-irradiated samples (p < 0.05). The zeta potential values of GNRs, PSS-GNRs and DOX-PSS-GNRs were measured as 42 ± 0.1 mV, −40 ± 0.3 mV and 39.3 ± 0.6 mV, respectively. PSS-GNRs nanocomplexes were found to be biocompatible and showed higher photothermal stability. The DOX-conjugated nanocomplexes with NIR laser irradiation appear more efficient in cell inhibition (93%) than those without laser exposure (65%) and doxorubicin alone (84%). The IC50 values of PSS-GNRs-DOX and PSS-GNRs-DOX were measured as 7.99 and 3.12 µg/mL, respectively, with laser irradiation. Thus, a combinatorial approach based on chemotherapy and photothermal strategies appears to be a promising platform in cancer management.

Laser exposure of gold nanorods can induce intracellular calcium transients

2013 ◽  
Vol 7 (10) ◽  
pp. 761-765 ◽  
Author(s):  
Chiara Paviolo ◽  
John W. Haycock ◽  
Peter J. Cadusch ◽  
Sally L. McArthur ◽  
Paul R. Stoddart
Keyword(s):  
Intracellular Calcium ◽  
Gold Nanorods ◽  
Calcium Transients ◽  
Laser Exposure

Chronic Laser Exposure Effects on Rabbit Retina

1972 ◽  
Vol 30 ◽  
pp. 54-55
Author(s):  
Burton B. Silver ◽  
Theodore Lawwill
Keyword(s):  
Laser Irradiation ◽  
Laser Light ◽  
Broad Band ◽  
Argon Laser ◽  
Atropine Sulfate ◽  
Ultrastructural Changes ◽  
Laser Exposure ◽  
Rabbit Retina ◽  
Histological Changes ◽  
Threshold Doses

Dutch-belted 1 to 2.5 kg anesthetized rabbits were exposed to either xenon or argon laser light administered in a broad band, designed to cover large areas of the retina. For laser exposure, the pupil was dilated with atropine sulfate 1% and pheny lephrine 10%. All of the laser generated power was within a band centered at 5145.0 Anstroms. Established threshold for 4 hour exposures to laser irradiation are in the order of 25-35 microwatts/cm2. Animals examined for ultrastructural changes received 4 hour threshold doses. These animals exhibited ERG, opthalmascopic, and histological changes consistent with threshold damage.One month following exposure the rabbits were killed with pentobarbitol. The eyes were immediately enucleated and dissected while bathed in 3% phosphate buffered gluteraldehyde.

The Red Neuronal Pigment in the Nervous System of the Mussel, Mytilus Edulis: Localization and Its Effect on Lateral Ciliary Activity with Spectral and Analytical Changes

1981 ◽  
Vol 39 ◽  
pp. 494-495
Author(s):  
Anthony A. Paparo ◽  
Judith A. Murphy
Keyword(s):  
Nervous System ◽  
Mytilus Edulis ◽  
Neuronal Cell ◽  
Ciliary Activity ◽  
Neuronal Cell Bodies ◽  
The Central Nervous System ◽  
Intracellular Organelles ◽  
The Common ◽  
The Individual ◽  
Cryostat Sections

The purpose of this study was to localize the red neuronal pigment in Mytilus edulis and examine its role in the control of lateral ciliary activity in the gill. The visceral ganglia (Vg) in the central nervous system show an over al red pigmentation. Most red pigments examined in squash preps and cryostat sec tions were localized in the neuronal cell bodies and proximal axon regions. Unstained cryostat sections showed highly localized patches of this pigment scattered throughout the cells in the form of dense granular masses about 5-7 um in diameter, with the individual granules ranging from 0.6-1.3 um in diame ter. Tissue stained with Gomori's method for Fe showed bright blue granular masses of about the same size and structure as previously seen in unstained cryostat sections.Thick section microanalysis (Fig.l) confirmed both the localization and presence of Fe in the nerve cell. These nerve cells of the Vg share with other pigmented photosensitive cells the common cytostructural feature of localization of absorbing molecules in intracellular organelles where they are tightly ordered in fine substructures.

Fading of the diaminobenzidine reaction product in the confocal scanning laser microscope

1989 ◽  
Vol 47 ◽  
pp. 146-147
Author(s):  
JS Deitch ◽  
KL Smith ◽  
JW Swann ◽  
JN Turner
Keyword(s):  
Pyramidal Neurons ◽  
Light And Electron Microscopy ◽  
Scanning Laser ◽  
Laser Exposure ◽  
Confocal Scanning Laser Microscope ◽  
Fluorescent Markers ◽  
Before And After ◽  
Staining Protocol ◽  
Confocal Scanning ◽  
Confocal Scanning Laser

Neurons labeled with horseradish peroxidase and reacted with diaminobenzidine (DAB) can be imaged using a confocal scanning laser microscope (CSLM) in the reflection mode. In contrast to fluorescent markers, the DAB reaction product is thought to be stable and can be observed by both light and electron microscopy. We have investigated the sensitivity of the DAB reaction product to laser irradiation, and present the spectrophotometric properties of DAB before and after exposure in the CSLM.Pyramidal neurons in slices of rat hippocampus were injected with biocytin (a biotin-lysine complex), fixed overnight in 4% paraformaldehyde, and vibratome sectioned at 75 μm. Biocytin was detected with avidin-HRP (1:200) in 0.5% Triton X-100, incubated in DAB (0.5 mg/ml) with or without 0.04% nickel ammonium sulfate (Ni), dehydrated, and imaged in a Bio Rad MRC-500 CSLM with an argon ion laser (488 and 514 nm). Spectrophotometric measurements of the soma were made on a Zeiss microspectrophotometer, as a function of laser exposure (100-1000 scans) and staining protocol.

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