Air sparging for prevention of antibody disulfide bond reduction in harvested CHO cell culture fluid

2014 ◽  
Vol 112 (4) ◽  
pp. 734-742 ◽  
Author(s):  
Melissa Mun ◽  
Stefanie Khoo ◽  
Aline Do Minh ◽  
James Dvornicky ◽  
Melody Trexler-Schmidt ◽  
...  
Keyword(s):  
Cell Culture ◽  
Disulfide Bond ◽  
Culture Fluid ◽  
Air Sparging ◽  
Cho Cell ◽  
Cell Culture Fluid ◽  
Cho Cell Culture ◽  
Disulfide Bond Reduction

AN EFFICIENCY COMPARISON OF CLARIFYING FILTERS DURIG THE HARVEST OF CHO CELL CULTURE FLUID

2020 ◽  
pp. 224-226
Author(s):  
N. Lobanova ◽  
I. Trusova ◽  
A. Kholodova ◽  
R. Marygin ◽  
Yu. Seregin
Keyword(s):  
Cell Culture ◽  
Culture Fluid ◽  
Cho Cell ◽  
Cell Culture Fluid ◽  
Cho Cell Culture ◽  
Efficiency Comparison

The turbidity of the culture fluid (CF) and the volume of clarified CF obtained by using nine different filters with the same area (25 cm2) and their combinations were compared.

scholarly journals Quantitative Risk assessment of product disulfide bond reduction in a recombinant protein manufacturing

2021 ◽  
Author(s):  
Yi Qing cui ◽  
rujie meng ◽  
yutong Li
Keyword(s):  
Risk Assessment ◽  
Cell Culture ◽  
Recombinant Protein ◽  
Disulfide Bond ◽  
Culture Fluid ◽  
Culture Conditions ◽  
Quantitative Risk Assessment ◽  
Prevention Measures ◽  
Recombinant Molecule ◽  
Disulfide Bond Reduction

Disulfide bond reduction occurred now and then during a recombinant protein manufacturing. Their reduction often led in the loss of batches. Various prevention measures were developed. Their implementation in a manufacturing inevitably increases its operation complexity and even might impact its product qualities and process performances. It is highly desirable to apply a reduction measure only if necessary and to apply no strong measure if a moderate one works. The reduction occurrence is determined both by the reduction sensitivity of a recombinant molecule and by the reduction power of harvested cell culture fluids. The reduction power of harvested cell culture fluids varies largely and depends on cells, lysis level, growth stage, and culture conditions. The method of the quantitative risk assessment of disulfide bond reduction was reported here. The quantitative assessment is realized by comparing the reduction sensitivity of a recombinant molecule with the reduction power of its harvested cell culture fluid.

scholarly journals Benchmarking of commercially available CHO cell culture media for antibody production

2015 ◽  
Vol 99 (11) ◽  
pp. 4645-4657 ◽  
Author(s):  
David Reinhart ◽  
Lukas Damjanovic ◽  
Christian Kaisermayer ◽  
Renate Kunert
Keyword(s):  
Cell Culture ◽  
Antibody Production ◽  
Culture Media ◽  
Cho Cell ◽  
Cell Culture Media ◽  
Cho Cell Culture

scholarly journals Equal mixing time enables scale‐down and optimisation of a CHO cell culture process using a shaken microbioreactor system

2021 ◽  
Author(s):  
Vincent Wiegmann ◽  
Richard A. Gardner ◽  
Daniel I. R. Spencer ◽  
Frank Baganz
Keyword(s):  
Cell Culture ◽  
Mixing Time ◽  
Cell Culture Process ◽  
Cho Cell ◽  
Scale Down ◽  
Culture Process ◽  
Cho Cell Culture

scholarly journals Reduction of charge variants by CHO cell culture process optimization

2020 ◽  
Vol 72 (2) ◽  
pp. 259-269 ◽  
Author(s):  
Zhibing Weng ◽  
Jian Jin ◽  
ChunHua Shao ◽  
Huazhong Li
Keyword(s):  
Cell Culture ◽  
Process Optimization ◽  
Cell Culture Process ◽  
Cho Cell ◽  
Culture Process ◽  
Cho Cell Culture ◽  
Charge Variants

CONTINUOUS SUSPENSION CHO CELL CULTURE

1994 ◽  
pp. 215-217
Author(s):  
Henrik Albahn Hansen ◽  
Claus Emborg
Keyword(s):  
Cell Culture ◽  
Cho Cell ◽  
Cho Cell Culture

An investigation of nucleotide sugar dynamics under the galactose supplementation in CHO cell culture

2019 ◽  
Vol 81 ◽  
pp. 165-174 ◽  
Author(s):  
Sha Sha ◽  
Seongkyu Yoon
Keyword(s):  
Cell Culture ◽  
Cho Cell ◽  
Nucleotide Sugar ◽  
Cho Cell Culture

Model‐based optimization of antibody galactosylation in CHO cell culture

2019 ◽  
Vol 116 (7) ◽  
pp. 1612-1626 ◽  
Author(s):  
Pavlos Kotidis ◽  
Philip Jedrzejewski ◽  
Si Nga Sou ◽  
Christopher Sellick ◽  
Karen Polizzi ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cho Cell ◽  
Model Based ◽  
Cho Cell Culture

scholarly journals Novel column generation-based optimization approach for poly-pathway kinetic model applied to CHO cell culture

2019 ◽  
Vol 8 ◽  
pp. e00083 ◽  
Author(s):  
Erika Hagrot ◽  
Hildur Æsa Oddsdóttir ◽  
Meeri Mäkinen ◽  
Anders Forsgren ◽  
Véronique Chotteau
Keyword(s):  
Cell Culture ◽  
Kinetic Model ◽  
Column Generation ◽  
Optimization Approach ◽  
Cho Cell ◽  
Cho Cell Culture
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