A mathematical model and computational framework for three-dimensional chondrocyte cell growth in a porous tissue scaffold placed inside a bi-directional flow perfusion bioreactor

2015 ◽  
Vol 112 (12) ◽  
pp. 2601-2610 ◽  
Author(s):  
Md. Shakhawath Hossain ◽  
D.J. Bergstrom ◽  
X.B. Chen
Keyword(s):  
Mathematical Model ◽  
Cell Growth ◽  
Three Dimensional ◽  
Perfusion Bioreactor ◽  
Computational Framework ◽  
Tissue Scaffold ◽  
Directional Flow ◽  
Flow Perfusion ◽  
Flow Perfusion Bioreactor

scholarly journals A Novel Flow-Perfusion Bioreactor Supports 3D Dynamic Cell Culture

2009 ◽  
Vol 2009 ◽  
pp. 1-7 ◽  
Author(s):  
Alexander M. Sailon ◽  
Alexander C. Allori ◽  
Edward H. Davidson ◽  
Derek D. Reformat ◽  
Robert J. Allen ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Density ◽  
Three Dimensional ◽  
Perfusion Bioreactor ◽  
Peripheral Cell ◽  
Cell Culture Techniques ◽  
Culture Techniques ◽  
Flow Perfusion ◽  
Flow Perfusion Bioreactor

Background. Bone engineering requires thicker three-dimensional constructs than the maximum thickness supported by standard cell-culture techniques (2 mm). A flow-perfusion bioreactor was developed to provide chemotransportation to thick (6 mm) scaffolds.Methods. Polyurethane scaffolds, seeded with murine preosteoblasts, were loaded into a novel bioreactor. Control scaffolds remained in static culture. Samples were harvested at days 2, 4, 6, and 8 and analyzed for cellular distribution, viability, metabolic activity, and density at the periphery and core.Results. By day 8, static scaffolds had a periphery cell density of , while in the core it was . Flow-perfused scaffolds demonstrated peripheral cell density of and core density of at day 8.Conclusions. Flow perfusion provides chemotransportation to thick scaffolds. This system may permit high throughput study of 3D tissues in vitro and enable prefabrication of biological constructs large enough to solve clinical problems.

scholarly journals Flow-perfusion bioreactor system for engineered breast cancer surrogates to be used in preclinical testing

2015 ◽  
Vol 11 (4) ◽  
pp. 1242-1250 ◽  
Author(s):  
Lauren E. Marshall ◽  
Kayla F. Goliwas ◽  
Lindsay M. Miller ◽  
Andrew D. Penman ◽  
Andra R. Frost ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Perfusion Bioreactor ◽  
Preclinical Testing ◽  
Flow Perfusion ◽  
Bioreactor System ◽  
Flow Perfusion Bioreactor
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