scholarly journals Protein release fromEscherichia coli cells permeabilized with guanidine-HCl and Triton X100

1989 ◽  
Vol 33 (7) ◽  
pp. 886-895 ◽  
Author(s):  
David Hettwer ◽  
Henry Wang
Keyword(s):  
Protein Release ◽  
Fromescherichia Coli ◽  
Triton X100

scholarly journals Rapid protein release fromEscherichia coli by chemical permeabilization under fermentation conditions

1992 ◽  
Vol 39 (7) ◽  
pp. 732-740 ◽  
Author(s):  
Thomas J. Naglak ◽  
Henry Y. Wang
Keyword(s):  
Protein Release ◽  
Fermentation Conditions ◽  
Fromescherichia Coli ◽  
Chemical Permeabilization

XYLANASE, AN ENVIRONMENTALLY FRIENDLY BLEACHING AGENT FOR PULP AND PAPER: Characterization And Stabilization Study Of Bacillus licheniformis I-5 CRUDE Xylanase

2018 ◽  
Vol 7 (3) ◽  
Author(s):  
Budiasih Wahyuntari., dkk
Keyword(s):  
Thermal Stability ◽  
Bacillus Licheniformis ◽  
Hot Spring ◽  
Sodium Dodecyl ◽  
Luria Broth ◽  
Crude Enzyme ◽  
Half Time ◽  
Triton X100 ◽  
Sds Page ◽  
Xylanolytic Enzymes

Isolate I-5 was isolated from Ciseeng hot spring, West Java and was identified as Bacillus licheniformis I-5. The isolate produces extracellular xylanolytic enzymes on Oatspelt containing Luria broth agar medium. Optimal activity of the crude enzyme was  observed at 50ºC and pH 7. The effect of sodium dodecyl sulphate, b-mercaptoethanol and Triton-X100 were observed. Incubating the crude enzyme in 1.5% SDS and 1.5% b-mercaptoethanol at 50oC for 90 minutes then adding Triton-X100 at final concentration of 3.5% for 45 minutes only reduced 5.75% of the initial enzyme activity. SDS/PAGE and zymogram analysis showed that at least two xylanolytic enzymes presence in the crude enzyme. The molecular weight of the enzyme was estimated about 127 and 20kD. The enzyme hydrolysed xylan into xylobiose, xylotriose and other longer xylooligosaccharides. Thermal stability of the crude enzyme was observed at 50, 60, and 70oC and pH 7 and 8. The results showed that the half time of the crude enzyme incubated at 50, 60, and 70oC pH 7 was 2 hours 55 minutes; 2 hours 33 minutes and 1 hour 15 minutes respectively. The half time at 50, 60 and 70oC, pH 8 was 2 hours 48 minutes; 1 hour 22 minutes and 1 hour 9 minutes respectively.keywords: Xilanase, Bacillus licheniformis I-5, thermal stability

Polymer chemistry effects on protein release and immune activation

2008 ◽  
Author(s):  
Senja Katarina Lopac
Keyword(s):  
Immune Activation ◽  
Protein Release ◽  
Polymer Chemistry

Crystallization and preliminary X-ray analysis of ferric enterobactin receptor FepA, an integral membrane protein fromEscherichia coli

1998 ◽  
Vol 54 (4) ◽  
pp. 697-699 ◽  
Author(s):  
Barbara S. Smith ◽  
Bostjan Kobe ◽  
Ravi Kurumbail ◽  
Susan K. Buchanan ◽  
Lalitha Venkatramani ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Integral Membrane Protein ◽  
X Ray ◽  
Fromescherichia Coli

C-terminal His-tagging results in substrate specificity changes of the thioesterase I fromEscherichia coli

1999 ◽  
Vol 76 (10) ◽  
pp. 1113-1118 ◽  
Author(s):  
Ya-Lin Lee ◽  
May-Shyuan Su ◽  
Tai-Huang Huang ◽  
Jei-Fu Shaw
Keyword(s):  
Substrate Specificity ◽  
Fromescherichia Coli

scholarly journals Primary sequence of glutamic acid tRNA II fromEscherichia coli

FEBS Letters ◽  
1972 ◽  
Vol 20 (2) ◽  
pp. 239-241 ◽  
Author(s):  
Ziro Ohashi ◽  
Fumio Harada ◽  
Susumu Nishimura
Keyword(s):  
Glutamic Acid ◽  
Primary Sequence ◽  
Fromescherichia Coli

scholarly journals Structure of the aliphatic sulfonate-binding protein SsuA fromEscherichia coli

2010 ◽  
Vol 66 (4) ◽  
pp. 391-396 ◽  
Author(s):  
John Beale ◽  
Sang Yun Lee ◽  
So Iwata ◽  
Konstantinos Beis
Keyword(s):  
Binding Protein ◽  
Fromescherichia Coli
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