One-step synthesis of protein-encapsulated microspheres in a porous scaffold by freeze-drying double emulsions and tuneable protein release

The microstructure of scaffold was one of key factors for tissue engineering. Porous polycaprolactone (PCL) scaffolds were fabricated by combination of porogen-leaching and freeze-drying process. Ice particulates were used as porogen material, and PCL solutions in chloroform were mixed with ice particulates for 5minuture at zero temperature. Then the mixture was freezed in liquid nitrogen, and porous scaffold was prepared by freeze - drying finally. The microstructure and properties of the scaffolds were investigated. Porous structure of the scaffolds showed that good 3D microstructure and no porogen remained in the scaffold; pore size and porosity were determined by the size and mass fraction of ice particulates. The results demonstrated that the Scaffolds possessed open and interconnected pores with sizes ranging from several μm to more than 300μm and porosities of 50~80%.

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One-Step Microfluidic Fabrication of Polyelectrolyte Microcapsules in Aqueous Conditions for Protein Release

Angewandte Chemie International Edition ◽

10.1002/anie.201606960 ◽

2016 ◽

Vol 55 (43) ◽

pp. 13470-13474 ◽

Cited By ~ 54

Author(s):

Liyuan Zhang ◽

Li-Heng Cai ◽

Philipp S. Lienemann ◽

Torsten Rossow ◽

Ingmar Polenz ◽

...

Keyword(s):

Protein Release ◽

Polyelectrolyte Microcapsules ◽

One Step ◽

Aqueous Conditions

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Freeze Drying of Double Emulsions to Prepare Topotecan-Entrapping Liposomes Featuring Controlled Release

Drug Development and Industrial Pharmacy ◽

10.1080/03639040701743980 ◽

2008 ◽

Vol 34 (4) ◽

pp. 427-433 ◽

Cited By ~ 3

Author(s):

Ting Wang ◽

Ning Wang ◽

Tiefu Li ◽

Yingjie Deng

Keyword(s):

Controlled Release ◽

Freeze Drying ◽

Double Emulsions

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A femoral shape porous scaffold bio-nanocomposite fabricated using 3D printing and freeze-drying technique for orthopedic application

Materials Chemistry and Physics ◽

10.1016/j.matchemphys.2021.125302 ◽

2022 ◽

Vol 275 ◽

pp. 125302

Author(s):

Xiaobiao Du ◽

Mohammad Dehghani ◽

Naif Alsaadi ◽

Mazyar Ghadiri Nejad ◽

Saeed Saber-Samandari ◽

...

Keyword(s):

3D Printing ◽

Freeze Drying ◽

Porous Scaffold

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Some Fundamentals of the One-Step Formation of Double Emulsions

Journal of Dispersion Science and Technology ◽

10.1080/01932690903224656 ◽

2010 ◽

Vol 31 (8) ◽

pp. 1019-1026 ◽

Cited By ~ 16

Author(s):

Jacqueline M. Morais ◽

Orlando D. H. Santos ◽

Stig E. Friberg

Keyword(s):

Double Emulsions ◽

One Step ◽

The One

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Coaxial oblique interface shearing: tunable generation and sorting of double emulsions for spatial gradient drug release

Lab on a Chip ◽

10.1039/d0lc00111b ◽

2020 ◽

Vol 20 (7) ◽

pp. 1249-1258 ◽

Cited By ~ 1

Author(s):

Fangsheng Huang ◽

Zhiqiang Zhu ◽

Ye Niu ◽

Yi Zhao ◽

Ting Si ◽

...

Keyword(s):

Drug Release ◽

Spatial Gradient ◽

Double Emulsions ◽

One Step ◽

Interface Shearing

We propose a coaxial oblique interface shearing (COIS) process to one-step generate and sort double emulsions for thermal- and chemical-responsive spatial gradient releases.

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