scholarly journals Rapid protein release fromEscherichia coli by chemical permeabilization under fermentation conditions

1992 ◽  
Vol 39 (7) ◽  
pp. 732-740 ◽  
Author(s):  
Thomas J. Naglak ◽  
Henry Y. Wang
Keyword(s):  
Protein Release ◽  
Fermentation Conditions ◽  
Fromescherichia Coli ◽  
Chemical Permeabilization

scholarly journals Protein release fromEscherichia coli cells permeabilized with guanidine-HCl and Triton X100

1989 ◽  
Vol 33 (7) ◽  
pp. 886-895 ◽  
Author(s):  
David Hettwer ◽  
Henry Wang
Keyword(s):  
Protein Release ◽  
Fromescherichia Coli ◽  
Triton X100

Optimization of fermentation conditions for the production of recombinant hpdgf-bb in Pichia pastoris

2015 ◽  
Vol 37 (1se) ◽  
Author(s):  
Duong Long Duy ◽  
Pham Minh Vu ◽  
Nguyen Tri Nhan ◽  
Tran Linh Thuoc ◽  
Dang Thi Phuong Thao
Keyword(s):  
Pichia Pastoris ◽  
Fermentation Conditions ◽  
Optimization Of Fermentation

Polymer chemistry effects on protein release and immune activation

2008 ◽  
Author(s):  
Senja Katarina Lopac
Keyword(s):  
Immune Activation ◽  
Protein Release ◽  
Polymer Chemistry

Effect of Crude Polysaccharide Isolated from Mageoli Mash under Different Fermentation Conditions on Immune-Stimulating Activity

2014 ◽  
Vol 43 (12) ◽  
pp. 1835-1842
Author(s):  
Young Kyoung Rhee ◽  
So-Hyun Nam ◽  
Hye-Ryun Kim ◽  
Chang-Won Cho ◽  
Young-Chul Lee ◽  
...  
Keyword(s):  
Fermentation Conditions ◽  
Crude Polysaccharide

Optimal Lactic Acid Fermentation Conditions and Quality Properties for Rubus coreanus Miquel (Bokbunja) and Chlorella Mixtures

2016 ◽  
Vol 45 (3) ◽  
pp. 386-395
Author(s):  
Jae-Young Kim ◽  
Sang-Uk Lee ◽  
Na-Hyung Kim ◽  
Kwang-Hyun Moon ◽  
Seung-Hwa Baek
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Fermentation ◽  
Acid Fermentation ◽  
Fermentation Conditions ◽  
Rubus Coreanus ◽  
Quality Properties ◽  
Rubus Coreanus Miquel

Crystallization and preliminary X-ray analysis of ferric enterobactin receptor FepA, an integral membrane protein fromEscherichia coli

1998 ◽  
Vol 54 (4) ◽  
pp. 697-699 ◽  
Author(s):  
Barbara S. Smith ◽  
Bostjan Kobe ◽  
Ravi Kurumbail ◽  
Susan K. Buchanan ◽  
Lalitha Venkatramani ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Integral Membrane Protein ◽  
X Ray ◽  
Fromescherichia Coli

scholarly journals An Assessment of the Viability of Lytic Phages and Their Potency against Multidrug Resistant Escherichia coli O177 Strains under Simulated Rumen Fermentation Conditions

Antibiotics ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 265
Author(s):  
Peter Kotsoana Montso ◽  
Caven Mguvane Mnisi ◽  
Collins Njie Ateba ◽  
Victor Mlambo
Keyword(s):  
Escherichia Coli ◽  
Rumen Fluid ◽  
Multidrug Resistant ◽  
Rumen Fermentation ◽  
Ruminal Fermentation ◽  
Fermentation Conditions ◽  
E Coli ◽  
Cell Counts ◽  
Milk Contamination ◽  
Novel Strategy

Preslaughter starvation and subacute ruminal acidosis in cattle are known to promote ruminal proliferation of atypical enteropathogenic Escherichia coli strains, thereby increasing the risk of meat and milk contamination. Using bacteriophages (henceforth called phages) to control these strains in the rumen is a potentially novel strategy. Therefore, this study evaluated the viability of phages and their efficacy in reducing E. coli O177 cells in a simulated ruminal fermentation system. Fourteen phage treatments were allocated to anaerobic serum bottles containing a grass hay substrate, buffered (pH 6.6–6.8) bovine rumen fluid, and E. coli O177 cells. The serum bottles were then incubated at 39 °C for 48 h. Phage titres quadratically increased with incubation time. Phage-induced reduction of E. coli O177 cell counts reached maximum values of 61.02–62.74% and 62.35–66.92% for single phages and phage cocktails, respectively. The highest E. coli O177 cell count reduction occurred in samples treated with vB_EcoM_366B (62.31%), vB_EcoM_3A1 (62.74%), vB_EcoMC3 (66.67%), vB_EcoMC4 (66.92%), and vB_EcoMC6 (66.42%) phages. In conclusion, lytic phages effectively reduced E. coli O177 cells under artificial rumen fermentation conditions, thus could be used as a biocontrol strategy in live cattle to reduce meat and milk contamination in abattoirs and milking parlours, respectively.

scholarly journals Metabolomic Analysis of The Chemical Diversity of South Africa Leaf Litter Fungal Species Using an Epigenetic Culture-Based Approach

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4262
Author(s):  
Rachel Serrano ◽  
Víctor González-Menéndez ◽  
Germán Martínez ◽  
Clara Toro ◽  
Jesús Martín ◽  
...  
Keyword(s):  
South Africa ◽  
Genome Mining ◽  
Fungal Species ◽  
Chemical Diversity ◽  
Fermentation Conditions ◽  
Metabolite Profiles ◽  
Different Populations ◽  
Fungal Genomes ◽  
Microbial Natural Products ◽  
Fermentation Media

Microbial natural products are an invaluable resource for the biotechnological industry. Genome mining studies have highlighted the huge biosynthetic potential of fungi, which is underexploited by standard fermentation conditions. Epigenetic effectors and/or cultivation-based approaches have successfully been applied to activate cryptic biosynthetic pathways in order to produce the chemical diversity suggested in available fungal genomes. The addition of Suberoylanilide Hydroxamic Acid to fermentation processes was evaluated to assess its effect on the metabolomic diversity of a taxonomically diverse fungal population. Here, metabolomic methodologies were implemented to identify changes in secondary metabolite profiles to determine the best fermentation conditions. The results confirmed previously described effects of the epigenetic modifier on the metabolism of a population of 232 wide diverse South Africa fungal strains cultured in different fermentation media where the induction of differential metabolites was observed. Furthermore, one solid-state fermentation (BRFT medium), two classic successful liquid fermentation media (LSFM and YES) and two new liquid media formulations (MCKX and SMK-II) were compared to identify the most productive conditions for the different populations of taxonomic subgroups.

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