A new method for the adaptive determination of optimum pH and temperature

1989 ◽  
Vol 33 (11) ◽  
pp. 1419-1424 ◽  
Author(s):  
Jeff. L Harmon ◽  
Gerasimos Lyberatos ◽  
Spyros A. Svoronos
Keyword(s):  
New Method ◽  
Optimum Ph ◽  
Optimum Ph And Temperature

A new method for the adaptive determination of optimum pH and temperature

1989 ◽  
Vol 34 (7) ◽  
pp. 1022-1022
Author(s):  
Jeff. L. Harmon ◽  
Gerasimos Lyberatos ◽  
Spyros A. Svoronos
Keyword(s):  
New Method ◽  
Optimum Ph ◽  
Optimum Ph And Temperature

scholarly journals A new method for the quantitative determination of monosaccharides, amino sugars and N-acetylneuraminic acid and of 6-deoxyhexose (fucose) in the presence of other sugars

1968 ◽  
Vol 110 (3) ◽  
pp. 413-417 ◽  
Author(s):  
Gwyneth M. Brearley ◽  
Jacqueline B. Weiss
Keyword(s):  
Quantitative Determination ◽  
Colorimetric Assay ◽  
Periodate Oxidation ◽  
New Method ◽  
Amino Sugars ◽  
Simple Method ◽  
Acetylneuraminic Acid ◽  
Assay Procedure ◽  
Optimum Ph

1. Monosaccharides, amino sugars and N-acetylneuraminic acid were determined by using an original colorimetric assay procedure, based on the detection of formaldehyde released after periodate oxidation. A range of these compounds was investigated by this method and they were all found to obey Beer's law within the concentration range 0–0·6μmole/ml. 2. A simple method for the determination of 6-deoxyhexose concentration in the presence of other monosaccharides is also described. 3. The optimum pH for the release of formaldehyde from sugars by periodate oxidation was 7·0–7·5. 4. The methods described have considerable advantages over existing assay systems and their particlar value in automatic colorimetry, where the use of concentrated acids is undesirable, is discussed.

scholarly journals Utilization of Crude Intracellular Chitinase Enzyme from Providencia stuartii for Glucosamine Production from Shrimp Shells

REAKTOR ◽  
2019 ◽  
Vol 19 (2) ◽  
pp. 62-67
Author(s):  
Hardoko Hardoko ◽  
Titri Siratantri Mastuti ◽  
Desy Puspasari ◽  
Yuniwaty Halim
Keyword(s):  
Optimum Condition ◽  
Substrate Concentration ◽  
Intracellular Enzyme ◽  
Fermentation Time ◽  
Shrimp Shells ◽  
Optimum Ph ◽  
Chitin Hydrolysis ◽  
Providencia Stuartii ◽  
Optimum Ph And Temperature

Chitin hydrolysis using enzyme is one of the methods to produce glucosamine in shorter time compared to using microbial cells, but the ability to produce glucosamine at enzyme’s optimum condition is influenced by substrate concentration and fermentation time. The objective of this research was to determine the optimum substrate concentration and fermentation time of shrimp shells’ chitin to produce glucosamine at the optimum pH and temperature of crude intracellular chitinase enzyme from Providencia stuartii. Method used was experimental method, started by extraction of intracellular enzyme from P. stuartii, followed by determination of optimum pH and temperature of enzyme. The optimum condition was used for experiment of shrimp shells’ chitin fermentation with treatments of chitin substrate concentration (0.5; 1.0; 1.5; 2.0%) and fermentation time (2, 4, 6 and 24 hours). Results showed that optimum enzyme activity occurred at pH of 5.0 and temperature of 40oC, which was about 6.03 U/ml. Concentration of chitin substrate and fermentation time influenced the amount of glucosamine obtained. Fermentation of shrimp shells’ chitin using crude intracellular enzyme was optimum at 1.0% substrate concentration and 6 hours fermentation time, which produced glucosamine about 1680.06±58.49 ppm. Keywords: intracellular chitinase enzyme, glucosamine, shrimp shells’ chitin, P. stuartii

A new method for the determination of nitrates

1960 ◽  
Vol 23 ◽  
pp. 227-232 ◽  
Author(s):  
P WEST ◽  
G LYLES
Keyword(s):  
New Method

A New Method for the Determination of Plasma Activators of Fibrinolysis

1977 ◽  
Vol 37 (02) ◽  
pp. 210-215 ◽  
Author(s):  
R Margalit ◽  
E Gidron ◽  
Y Shalitin
Keyword(s):  
New Method ◽  
Effective Activator

SummaryThe term “effective activator” of plasminogen is proposed, to denote the resultant of activator-antiactivator interaction, and a method for the determination of the level of these activators is described. By adding axcess plasminogen to the euglobulin fraction of plasma the influence of the level of endogenous plasminogen and of the antiplasmin is eliminated. It is shown that the level of fibrinogen has very little bearing on the results. An effective activator unit is defined as equal to 1 CTA unit of urokinase activity on a fibrinogen-plasminogen substrate.

The Plasmin Inhibitors of Plasma

1964 ◽  
Vol 12 (01) ◽  
pp. 119-125 ◽  
Author(s):  
Y Shamash ◽  
A Rimon
Keyword(s):  
Human Plasma ◽  
New Method ◽  
Normal Amount ◽  
Caseinolytic Activity ◽  
Before And After ◽  
Plasmin Inhibitors

SummaryA new method for the assay of plasmin inhibitors in human plasma is described. The method consists of determination of the caseinolytic activity of a standard plasmin solution before and after incubation with the inhibitor, with lysine added to the mixture as a stabilizer of plasmin. Using this method, it was found that plasma contains enough inhibitors to inactivate 30 caseinolytic units of plasmin, or 10 times the normal amount of plasminogen in human plasma.

AGGLUTINATION INHIBITION REACTIONS FOR THE DETERMINATION OF GONADOTROPHINS

1969 ◽  
Vol 62 (1_Suppl) ◽  
pp. S95-S112 ◽  
Author(s):  
A. H. W. M. Schuurs
Keyword(s):  
Immune System ◽  
Quantitative Determination ◽  
Haemagglutination Inhibition ◽  
Latex Agglutination ◽  
Literature Survey ◽  
New Method ◽  
Test Fluid ◽  
Main Application ◽  
Test Systems

ABSTRACT Various techniques for sensitising erythrocytes and latex particles with gonadotrophins, particularly with HCG, are described. The haemagglutination inhibition reactions are generally interpreted by means of »erythrocyte settling patterns«. By a new method of evaluating these patterns a relatively precise quantitative determination is possible. Latex agglutination inhibition reactions on slides are particularly suitable as rapid qualitative tests. In cases where the maximum attainable sensitivity of the agglutination inhibition tests is insufficient, e. g. for determining LH concentrations in urine, the hormone in the test fluid has to be concentrated or extracted. An alternative method is a modified haemagglutination inhibition test for large volumes which is applicable to unconcentrated urine. Due to non-specific inhibitions the above-mentioned tests cannot be applied to unprocessed serum. Agglutination inhibition tests with HCG are already well advanced, pregnancy diagnosis being their main application. Now that highly purified HCG is available, a satisfactory specificity for these tests can be attained. If the immune system for HCG is used for estimating LH, it has to meet additional specificity requirements. Furthermore, the measure of cross-reaction and the choice of standard merit special attention. Finally, a literature survey is given of test systems in which LH and FSH were used as antigens.

Development of a New Method for Determination of the Oil Content from Microalgae Lipid Fraction

2017 ◽  
Vol 68 (4) ◽  
pp. 671-674
Author(s):  
Ana Maria Galan ◽  
Ioan Calinescu ◽  
Elena Radu ◽  
Elena Emilia Oprescu ◽  
Gabriel Vasilievici ◽  
...  
Keyword(s):  
Sunflower Oil ◽  
Oil Content ◽  
Lipid Fraction ◽  
New Method ◽  
Ms Analysis ◽  
Qualitative Determination ◽  
Tga Analysis

The purpose of this study was to develop a method for rapid quantitative and qualitative determination of the oil from microalgae lipid fraction obtained from Nannochloris sp biomass. The lipid fraction was first refluxed with 4% KOH in MeOH (60, 90, 120 min), followed by reaction with 20% BF3 in MeOH, using different times of reflux (90,120, 150 min) for each time of reflux with 4% KOH in MeOH. The FAME samples were analyzed by GC-MS analysis. 120 min reflux with 4% KOH in MeOH, 90 min with 20% BF3 in MeOH and a ratio lipid fraction: 4% KOH in MeOH: 20% BF3 in MeOH=1:20:27, were required to obtain the higher percent of oil in the microalgae lipid fraction. The relevance of the method developed was proved by TGA analysis and by transesterification of a sunflower oil sample in the same conditions.

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