Kinetics of growth and elemental sulfur oxidation in batch culture ofthiobacillus ferrooxidans

1994 ◽  
Vol 44 (6) ◽  
pp. 667-673 ◽  
Author(s):  
Yasuhiro Konishi ◽  
Yuichiro Takasaka ◽  
Satoru Asai
2020 ◽  
Vol 8 (3) ◽  
pp. 386 ◽  
Author(s):  
Maxim Muravyov ◽  
Anna Panyushkina

A two-step process, which involved ferric leaching with biologically generated solution and subsequent biooxidation with the microbial community, has been previously proposed for the processing of low-grade zinc sulfide concentrates. In this study, we carried out the process of complete biological oxidation of the product of ferric leaching of the zinc concentrate, which contained 9% of sphalerite, 5% of chalcopyrite, and 29.7% of elemental sulfur. After 21 days of biooxidation at 40 °C, sphalerite and chalcopyrite oxidation reached 99 and 69%, respectively, while the level of elemental sulfur oxidation was 97%. The biooxidation residue could be considered a waste product that is inert under aerobic conditions. The results of this study showed that zinc sulfide concentrate processing using a two-step treatment is efficient and promising. The microbial community, which developed during biooxidation, was dominated by Acidithiobacillus caldus, Leptospirillum ferriphilum, Ferroplasma acidiphilum, Sulfobacillus thermotolerans, S. thermosulfidooxidans, and Cuniculiplasma sp. At the same time, F. acidiphilum and A. caldus played crucial roles in the oxidation of sulfide minerals and elemental sulfur, respectively. The addition of L. ferriphilum to A. caldus during biooxidation of the ferric leach product proved to inhibit elemental sulfur oxidation.


1998 ◽  
Vol 18 (4) ◽  
pp. 241 ◽  
Author(s):  
R. Gourdon ◽  
N. Funtowicz

2016 ◽  
Vol 89 ◽  
pp. 282-292 ◽  
Author(s):  
Mabel Mora ◽  
Luis R. López ◽  
Javier Lafuente ◽  
Julio Pérez ◽  
Robbert Kleerebezem ◽  
...  

Soil Research ◽  
2000 ◽  
Vol 38 (3) ◽  
pp. 753 ◽  
Author(s):  
A. D. Mitchell ◽  
P. Loganathan ◽  
T. W. Payn ◽  
R. W. Tillman

Application of Mg fertilisers has been suggested as a means of reducing the incidence of Mg deficiency of forest trees in New Zealand and Europe. The objective of this study was to determine the rates of dissolution of a range of Mg fertilisers applied to a pumice soil (Typic Udivitrand). The rate of fertiliser dissolution was little influenced by whether the fertiliser was applied directly on to the soil surface (litter removed) or on to the litter layer in a Pinus radiata plantation. Twenty-seven months since fertiliser application the mean (with and without litter) percentage of Mg dissolved was in the sequence: Epsom salts > calcined magnesite 1–2 mm > granmag (a partially acidulated and granulated calmag product) > calcined magnesite 2–4 mm > forestry grade dolomite. The specific dissolution rate constants (mg/cm2 .day of fertiliser) for the slowly soluble Mg fertilisers calculated using an elemental sulfur oxidation cubic model were 587 for calcined magnesite 1–2 mm, 426 for calcined magnesite 2–4 mm, 385 for granmag, and 18 for forestry grade dolomite. In a laboratory incubation study the elemental sulfur oxidation cubic model described the rate of dissolution of Mg fertilisers within narrow fertiliser particle size ranges. The specific fertiliser dissolution rate constants, however, increased with decreases in particle size, suggesting that the rate of dissolution depends on factors other than surface area when particle sizes varied widely. Slowly soluble, alkaline Mg fertilisers had a significant liming effect on the soil. They were more effective in increasing soil exchangeable Mg than soluble Mg salts over a long-period and therefore, they are better fertilisers for P. radiata.


1990 ◽  
Vol 70 (3) ◽  
pp. 499-502 ◽  
Author(s):  
H. H. JANZEN

Controlled environment studies were conducted to characterize the effects of cropping treatment and degree of particle dispersion on S oxidation rate. In two soils (a Chernozem and a Luvisol), S oxidation rates were not greatly affected by cropping treatment (barley, beans, canola, or fallow). In a second experiment, S oxidation was shown to approach maximum rates at a dispersion level of 1000 g soil g−1 S. Key words: Sulfur, placement, rhizosphere, fertilizer, elementals


1991 ◽  
Vol 37 (3) ◽  
pp. 182-187 ◽  
Author(s):  
Hector M. Lizama ◽  
Isamu Suzuki

The oxidation of elemental sulfur by two strains of Thiobacillus thiooxidans was studied by measuring the rate of O2 consumption at various concentrations of substrate and cells. In both the laboratory strain ATCC 8085 and the mine isolate SM-6, sulfur oxidation was competitively inhibited by T. thiooxidans cells; the Ki values were 0.65 and 0.05 mg wet cells∙mL−1, respectively. The rate constants were 500 and 143 μM O2∙min−1∙mg wet cells−1∙mL−1 and the Km values for sulfur concentration were 7.5 and 0.32% pulp density, respectively. Mine isolate SM-6 was used also to study pyrite (FeS2) oxidation by measuring the rate of O2 consumption. Oxidation of both washed and unwashed pyrite samples was competitively inhibited by increasing concentrations of cells; with each sample the Ki values was 0.05 mg wet cells∙mL−1. The rate constants for each sample were also the same (100 μM O2∙min−1∙mg wet cells−1∙mL−1), but the Km values were different (1.11% pulp density for washed pyrite and 2.81% pulp density for unwashed pyrite). Based on the rate of Fe solubilization from the washed pyrite sample, T. thiooxidans cells oxidized the sulfide released from pyrite dissolution beyond the oxidation state of elemental sulfur. Key words: Thiobacillus thiooxidans, sulfur, pyrite, oxidation, kinetics.


2007 ◽  
Vol 20-21 ◽  
pp. 584-584 ◽  
Author(s):  
Daniel Kupka ◽  
Mark Dopson ◽  
Olli H. Tuovinen

The purpose of this work was to characterize elemental sulfur oxidation by a psychrotrophic Acidithiobacillus ferrooxidans culture that originated from an AMD-impacted surface soil in a permafrost area in northern Siberia. In this work, the iron-oxidizing culture was cultivated with elemental sulfur with and without Fe2+ or Fe3+ in flasks on a shaker to avoid oxygen limitation.


Sign in / Sign up

Export Citation Format

Share Document