Characterization of a pH-inducible promoter system for high-level expression of recombinant proteins inEscherichia coli

1995 ◽  
Vol 47 (2) ◽  
pp. 186-192 ◽  
Author(s):  
Chih-Hsiung Chou ◽  
Aristos A. Aristidou ◽  
Shi-Yuan Meng ◽  
George N. Bennett ◽  
Ka-Yiu San
Keyword(s):  
Recombinant Proteins ◽  
Inducible Promoter ◽  
Inescherichia Coli ◽  
High Level Expression ◽  
High Level ◽  
Promoter System

scholarly journals First report of a tetracycline-inducible gene expression system for mollicutes

Microbiology ◽  
2010 ◽  
Vol 156 (1) ◽  
pp. 198-205 ◽  
Author(s):  
Marc Breton ◽  
Evelyne Sagné ◽  
Sybille Duret ◽  
Laure Béven ◽  
Christine Citti ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression System ◽  
Inducible Promoter ◽  
High Level Expression ◽  
Spiroplasma Citri ◽  
High Level ◽  
Tetr Repressor ◽  
Promoter System ◽  
Inducible Gene

Inducible promoter systems are powerful tools for studying gene function in prokaryotes but have never been shown to function in mollicutes. In this study we evaluated the efficacy of the tetracycline-inducible promoter Pxyl/tetO2 from Bacillus subtilis in controlling gene expression in two mollicutes, the plant pathogen Spiroplasma citri and the animal pathogen Mycoplasma agalactiae. An S. citri plasmid carrying the spiralin gene under the control of the xyl/tetO2 tetracycline-inducible promoter and the TetR repressor gene under the control of a constitutive spiroplasmal promoter was introduced into the spiralin-less S. citri mutant GII3-9a3. In the absence of tetracycline, expression of TetR almost completely abolished expression of spiralin from the xyl/tetO2 promoter. Adding tetracycline (>50 ng ml−1) to the medium induced high-level expression of spiralin. Interestingly, inducible expression of spiralin was also detected in vivo: in S. citri-infected leafhoppers fed on tetracycline-containing medium and in S. citri-infected plants watered with tetracycline. A similar construct was introduced into the M. agalactiae chromosome through transposition. Tetracycline-induced expression of spiralin proved the TetR-Pxyl/tetO2 system to be functional in the ruminant pathogen, suggesting that this tetracycline-inducible promoter system might be of general use in mollicutes.

High-level expression and characterization of Galactomyces geotrichum (BT107) lipase I in Pichia pastoris

2006 ◽  
Vol 49 (2) ◽  
pp. 256-264 ◽  
Author(s):  
Layla Fernández ◽  
Ignacio Pérez-Victoria ◽  
Alberto Zafra ◽  
Pedro L. Benítez ◽  
Juan C. Morales ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
High Level Expression ◽  
High Level

High-level expression and characterization of a newκ-carrageenase from marine bacteriumPedobacter hainanensisNJ-02

2018 ◽  
Vol 66 (5) ◽  
pp. 409-415 ◽  
Author(s):  
B.-W. Zhu ◽  
Q. Xiong ◽  
F. Ni ◽  
Y. Sun ◽  
Z. Yao
Keyword(s):  
High Level Expression ◽  
High Level
Sign in / Sign up

Export Citation Format

Share Document