Characterization of a pH-inducible promoter system for high-level expression of recombinant proteins inEscherichia coli

1995 ◽  
Vol 47 (2) ◽  
pp. 186-192 ◽  
Author(s):  
Chih-Hsiung Chou ◽  
Aristos A. Aristidou ◽  
Shi-Yuan Meng ◽  
George N. Bennett ◽  
Ka-Yiu San
Keyword(s):  
Recombinant Proteins ◽  
Inducible Promoter ◽  
Inescherichia Coli ◽  
High Level Expression ◽  
High Level ◽  
Promoter System

scholarly journals First report of a tetracycline-inducible gene expression system for mollicutes

Microbiology ◽  
2010 ◽  
Vol 156 (1) ◽  
pp. 198-205 ◽  
Author(s):  
Marc Breton ◽  
Evelyne Sagné ◽  
Sybille Duret ◽  
Laure Béven ◽  
Christine Citti ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression System ◽  
Inducible Promoter ◽  
High Level Expression ◽  
Spiroplasma Citri ◽  
High Level ◽  
Tetr Repressor ◽  
Promoter System ◽  
Inducible Gene

Inducible promoter systems are powerful tools for studying gene function in prokaryotes but have never been shown to function in mollicutes. In this study we evaluated the efficacy of the tetracycline-inducible promoter Pxyl/tetO2 from Bacillus subtilis in controlling gene expression in two mollicutes, the plant pathogen Spiroplasma citri and the animal pathogen Mycoplasma agalactiae. An S. citri plasmid carrying the spiralin gene under the control of the xyl/tetO2 tetracycline-inducible promoter and the TetR repressor gene under the control of a constitutive spiroplasmal promoter was introduced into the spiralin-less S. citri mutant GII3-9a3. In the absence of tetracycline, expression of TetR almost completely abolished expression of spiralin from the xyl/tetO2 promoter. Adding tetracycline (>50 ng ml−1) to the medium induced high-level expression of spiralin. Interestingly, inducible expression of spiralin was also detected in vivo: in S. citri-infected leafhoppers fed on tetracycline-containing medium and in S. citri-infected plants watered with tetracycline. A similar construct was introduced into the M. agalactiae chromosome through transposition. Tetracycline-induced expression of spiralin proved the TetR-Pxyl/tetO2 system to be functional in the ruminant pathogen, suggesting that this tetracycline-inducible promoter system might be of general use in mollicutes.

Cloning and high level expression of Cynodon dactylon (Bermuda grass) pollen profilin (Cyn d 12) in Escherichia coli: purification and characterization of the allergen

1997 ◽  
Vol 27 (11) ◽  
pp. 1307-1313 ◽  
Author(s):  
J. A. ASTURIAS ◽  
M. C. ARILLA ◽  
N. GOMEZ-BAYON ◽  
J. MARTINEZ ◽  
A. MARTINEZ ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Grass Pollen ◽  
Cynodon Dactylon ◽  
Bermuda Grass ◽  
Purification And Characterization ◽  
High Level Expression ◽  
Bermuda Grass Pollen ◽  
D 12 ◽  
High Level

High-level expression, purification and characterization of carbazole dioxygenase, a three components dioxygenase, of Pseudomonas GBS.5

2015 ◽  
Vol 37 (10) ◽  
pp. 1945-1952 ◽  
Author(s):  
Samiya Khan ◽  
D. K. Adhikari ◽  
Sanjay Gupta ◽  
Nidhi Gupta
Keyword(s):  
Purification And Characterization ◽  
High Level Expression ◽  
High Level ◽  
Three Components

scholarly journals High-level expression and characterization of a novel cutinase from Malbranchea cinnamomea suitable for butyl butyrate production

2017 ◽  
Vol 10 (1) ◽  
Author(s):  
Xiaojie Duan ◽  
Yu Liu ◽  
Xin You ◽  
Zhengqiang Jiang ◽  
Shaoxiang Yang ◽  
...  
Keyword(s):  
High Level Expression ◽  
Butyl Butyrate ◽  
High Level ◽  
Butyrate Production

Characterization of cDNA encoding mouse DNA repair protein O6-methylguanine-DNA methyltransferase and high-level expression of the wild-type and mutant proteins in E. coli

Biochemistry ◽  
1992 ◽  
Vol 31 (7) ◽  
pp. 1897-1903 ◽  
Author(s):  
Susumu Shiota ◽  
Mathew A. Von Wronski ◽  
Keizo Tano ◽  
Darell D. Bigner ◽  
Thomas P. Brent ◽  
...  
Keyword(s):  
Dna Methyltransferase ◽  
Wild Type ◽  
High Level Expression ◽  
Mutant Proteins ◽  
E Coli ◽  
Methylguanine Dna Methyltransferase ◽  
Repair Protein ◽  
Dna Repair Protein ◽  
High Level

High-level expression and characterization of Galactomyces geotrichum (BT107) lipase I in Pichia pastoris

2006 ◽  
Vol 49 (2) ◽  
pp. 256-264 ◽  
Author(s):  
Layla Fernández ◽  
Ignacio Pérez-Victoria ◽  
Alberto Zafra ◽  
Pedro L. Benítez ◽  
Juan C. Morales ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
High Level Expression ◽  
High Level

High-level expression of a novel α-galactosidase gene from Rhizomucor miehei in Pichia pastoris and characterization of the recombinant enyzme

2015 ◽  
Vol 110 ◽  
pp. 107-114 ◽  
Author(s):  
Zhou Chen ◽  
Qiaojuan Yan ◽  
Zhengqiang Jiang ◽  
Yu Liu ◽  
Yuchen Li
Keyword(s):  
Pichia Pastoris ◽  
Rhizomucor Miehei ◽  
High Level Expression ◽  
High Level

High-level expression and characterization of a newκ-carrageenase from marine bacteriumPedobacter hainanensisNJ-02

2018 ◽  
Vol 66 (5) ◽  
pp. 409-415 ◽  
Author(s):  
B.-W. Zhu ◽  
Q. Xiong ◽  
F. Ni ◽  
Y. Sun ◽  
Z. Yao
Keyword(s):  
High Level Expression ◽  
High Level
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