Characterization of cDNA encoding mouse DNA repair protein O6-methylguanine-DNA methyltransferase and high-level expression of the wild-type and mutant proteins in E. coli

Biochemistry ◽  
1992 ◽  
Vol 31 (7) ◽  
pp. 1897-1903 ◽  
Author(s):  
Susumu Shiota ◽  
Mathew A. Von Wronski ◽  
Keizo Tano ◽  
Darell D. Bigner ◽  
Thomas P. Brent ◽  
...  
Keyword(s):  
Dna Methyltransferase ◽  
Wild Type ◽  
High Level Expression ◽  
Mutant Proteins ◽  
E Coli ◽  
Methylguanine Dna Methyltransferase ◽  
Repair Protein ◽  
Dna Repair Protein ◽  
High Level

O6-methylguanine-DNA methyltransferase in wild-type and ada mutants of Escherichia coli

1982 ◽  
Vol 152 (1) ◽  
pp. 534-537
Author(s):  
S Mitra ◽  
B C Pal ◽  
R S Foote
Keyword(s):  
Escherichia Coli ◽  
Dna Methyltransferase ◽  
Wild Type ◽  
E Coli ◽  
Methylguanine Dna Methyltransferase ◽  
Synthetic Dna ◽  
In The Wild ◽  
Low Levels ◽  
Enzyme Molecules ◽  
Dna Substrate

O(6)-Methylguanine-DNA methyltransferase is induced in Escherichia coli during growth in low levels of N-methyl-N'-nitro-N-nitrosoguanidine. We have developed a sensitive assay for quantitating low levels of this activity with a synthetic DNA substrate containing 3H-labeled O(6)-methylguanine as the only modified base. Although both wild-type and adaptation-deficient (ada) mutants of E. coli contained low but comparable numbers (from 13 to 60) of the enzyme molecules per cell, adaptation treatment caused a significant increase of the enzyme in the wild type but not in the ada mutants, suggesting that the ada mutation is in a regulatory locus and not in the structural gene for the methyltransferase.

Characterization of O6-methylguanine-DNA methyltransferase in transgenic mice introduced with the E. coli ada gene

1991 ◽  
Vol 254 (3) ◽  
pp. 225-230 ◽  
Author(s):  
Yoko Nakatsuru ◽  
Shoichi Matsukuma ◽  
Mutsuo Sekiguchi ◽  
Takatoshi Ishikawa
Keyword(s):  
Transgenic Mice ◽  
Dna Methyltransferase ◽  
E Coli ◽  
Methylguanine Dna Methyltransferase
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