scholarly journals Decoupling of recombinant protein production from Escherichia coli cell growth enhances functional expression of plant Leloir glycosyltransferases

2019 ◽  
Vol 116 (6) ◽  
pp. 1259-1268 ◽  
Author(s):  
Martin Lemmerer ◽  
Juergen Mairhofer ◽  
Alexander Lepak ◽  
Karin Longus ◽  
Rainer Hahn ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Growth ◽  
Recombinant Protein ◽  
Recombinant Protein Production ◽  
Protein Production ◽  
Functional Expression ◽  
Coli Cell

scholarly journals Alleviation of Proteolytic Sensitivity To Enhance Recombinant Lipase Production in Escherichia coli

2009 ◽  
Vol 75 (16) ◽  
pp. 5424-5427 ◽  
Author(s):  
Niju Narayanan ◽  
C. Perry Chou
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Recombinant Protein ◽  
Recombinant Protein Production ◽  
Double Mutant ◽  
Protein Production ◽  
Functional Expression ◽  
Lipase Production ◽  
Recombinant Lipase ◽  
Pseudozyma Antarctica

ABSTRACT Two amino acids, Leu149 and Val223, were identified as proteolytically sensitive when Pseudozyma antarctica lipase (PalB) was heterologously expressed in Escherichia coli. The functional expression was enhanced using the double mutant for cultivation. However, the recombinant protein production was still limited by PalB misfolding, which was resolved by DsbA coexpression.

scholarly journals Isolating Escherichia coli strains for recombinant protein production

2016 ◽  
Vol 74 (5) ◽  
pp. 891-908 ◽  
Author(s):  
Susan Schlegel ◽  
Pierre Genevaux ◽  
Jan-Willem de Gier
Keyword(s):  
Escherichia Coli ◽  
Recombinant Protein ◽  
Recombinant Protein Production ◽  
Protein Production

Effect of E. Coli endotoxin on mammalian cell growth and recombinant protein production

1990 ◽  
Vol 26 (12) ◽  
pp. 1121-1122 ◽  
Author(s):  
J. Epstein ◽  
C. E. Kelly ◽  
M. M. Lee ◽  
P. K. Donahoe
Keyword(s):  
Cell Growth ◽  
Recombinant Protein ◽  
Mammalian Cell ◽  
Recombinant Protein Production ◽  
Protein Production ◽  
E Coli ◽  
Mammalian Cell Growth

scholarly journals Recombinant protein production in an Escherichia coli reduced genome strain

2007 ◽  
Vol 9 (2) ◽  
pp. 133-141 ◽  
Author(s):  
Shamik S. Sharma ◽  
Frederick R. Blattner ◽  
Sarah W. Harcum
Keyword(s):  
Escherichia Coli ◽  
Recombinant Protein ◽  
Recombinant Protein Production ◽  
Protein Production ◽  
Reduced Genome

Optimization of tetracycline‐responsive recombinant protein production and effect on cell growth and ER stress in mammalian cells

2005 ◽  
Vol 91 (6) ◽  
pp. 722-732 ◽  
Author(s):  
Jullian Jones ◽  
Tarangsri Nivitchanyong ◽  
Christina Giblin ◽  
Valentina Ciccarone ◽  
David Judd ◽  
...  
Keyword(s):  
Cell Growth ◽  
Recombinant Protein ◽  
Er Stress ◽  
Mammalian Cells ◽  
Recombinant Protein Production ◽  
Protein Production
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