Adsorbed Protein Film on Pump Surfaces Leads to Particle Formation During Fill‐Finish Manufacturing

AbstractPoly[bis(trifluoroethoxy)phosphazene] (PTFEP) with a high purity and high molar mass is a biocompatible material [1] used as bulk material in medical implants. We developed a process to coat surfaces with PTFEP films and performed ELISA experiments designed to understand their blood compatibility. We observed that PTFEP adsorbs preferentially albumin from plasma, and only small amounts of coagulation or inflammation stimulating proteins. In general, there is a good correlation between increasing content of albumin in the adsorbed protein film and reduced platelet adhesion. Another important prerequisite of blood compatibility is the stabilization of the native state of adsorbed proteins, since denaturated proteins stimulate platelet adhesion. The elutability of adsorbed proteins by sodiumdodecylsulfate solution was used to quantify the amount of irreversible attached and presumably denaturated proteins. PTFEP showed a low amount of irreversibly adsorbed proteins of the coagulation cascade. Circular dichroism measurements of adsorbed fibrinogen and albumin showed only weak distortions of the secondary structure of these proteins on the surface of PTFEP.We conclude that PTFEP has a unique blood compatibility because of the favorable composition and the stabilization of the protein layer against denaturation.

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Direct determination of the mechanical properties of an interfacially adsorbed protein film

Chemical Engineering Science ◽

10.1016/s0009-2509(02)00057-x ◽

2002 ◽

Vol 57 (10) ◽

pp. 1711-1722 ◽

Cited By ~ 30

Author(s):

Daniel B Jones ◽

Anton P.J Middelberg

Keyword(s):

Mechanical Properties ◽

Direct Determination ◽

Protein Film ◽

Adsorbed Protein

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Ion exchange kinetics in an adsorbed protein film

Bioelectrochemistry and Bioenergetics ◽

10.1016/0302-4598(76)85002-7 ◽

1976 ◽

Vol 3 (1) ◽

pp. 15-27 ◽

Cited By ~ 5

Author(s):

M. Blank ◽

W. Eisenberg ◽

J.S. Britten

Keyword(s):

Ion Exchange ◽

Protein Film ◽

Adsorbed Protein ◽

Ion Exchange Kinetics ◽

Exchange Kinetics

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A13 Surface Energy of Adsorbed protein film and its effect on frictional behavior of prosthetic joint materials

The Proceedings of Conference of Kyushu Branch ◽

10.1299/jsmekyushu.2012.5 ◽

2012 ◽

Vol 2012 (0) ◽

pp. 5-6

Author(s):

Kumiko HARA ◽

Kazuhiro NAKASHIMA ◽

Seido YARIMITSU ◽

Yoshinori SAWAE ◽

Teruo MURAKAMI

Keyword(s):

Surface Energy ◽

Protein Film ◽

Frictional Behavior ◽

Adsorbed Protein ◽

Prosthetic Joint

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Electron Microscopy of Nucleic Acids

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100051529 ◽

1974 ◽

Vol 32 ◽

pp. 302-303

Author(s):

Norman Davidson

Keyword(s):

Electron Microscopy ◽

Nucleic Acids ◽

Basic Protein ◽

Molecular Biologist ◽

Topological Properties ◽

Protein Film ◽

Polynucleotide Chain

The basic protein film technique for mounting nucleic acids for electron microscopy has proven to be a general and powerful tool for the working molecular biologist in characterizing different nucleic acids. It i s possible to measure molecular lengths of duplex and single-stranded DNAs and RNAs. In particular, it is thus possible to as certain whether or not the nucleic acids extracted from a particular source are or are not homogeneous in length. The topological properties of the polynucleotide chain (linear or circular, relaxed or supercoiled circles, interlocked circles, etc. ) can also be as certained.

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