scholarly journals Reducing phenotypic instabilities of a microbial population during continuous cultivation based on cell switching dynamics.

2021 ◽  
Author(s):  
Thai Minh Nguyen ◽  
Samuel Telek ◽  
Andrew Zicler ◽  
Juan Andres Martinez ◽  
Boris Zacchetti ◽  
...  
Keyword(s):  
Microbial Population ◽  
Continuous Cultivation ◽  
Cell Switching ◽  
Switching Dynamics

scholarly journals Reducing phenotypic instabilities of microbial population during continuous cultivation based on cell switching dynamics

2021 ◽  
Author(s):  
Thai Nguyen ◽  
Samuel Telek ◽  
Andrew Zicler ◽  
Juan Andres Martinez ◽  
Boris Zacchetti ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cell Population ◽  
Microbial Population ◽  
Phenotypic Diversity ◽  
Continuous Cultivation ◽  
Phenotypic Switching ◽  
Simultaneous Occurrence ◽  
Switching Dynamics ◽  
On Line ◽  
Cultivation Process

Predicting the fate of a microbial population (i.e., growth, gene expression…) remains a challenge, especially when this population is exposed to very dynamic environmental conditions, such as those encountered during continuous cultivation. Indeed, the dynamic nature of continuous cultivation process implies the potential deviation of the microbial population involving genotypic and phenotypic diversification. This work has been focused on the induction of the arabinose operon in Escherichia coli as a model system. As a preliminary step, the GFP level triggered by an arabinose-inducible ParaBAD promoter has been tracked by flow cytometry in chemostat with glucose-arabinose co-feeding. For a large range of glucose-arabinose co-feeding, the simultaneous occurrence of GFP positive and negative subpopulation was observed. In a second set of experiments, continuous cultivation was performed by adding either glucose or arabinose, based on the ability of individual cells for switching from low GFP to high GFP states, according to a technology called segregostat. In segregostat mode of cultivation, on-line flow cytometry analysis was used for adjusting the arabinose/glucose transitions based on the phenotypic switching capabilities of the microbial population. This strategy allowed finding an appropriate arabinose pulsing frequency, leading to a prolonged maintenance of the induction level with limited impact on phenotypic diversity for more than 60 generations. This result suggests that constraining individual cells into a given phenotypic trajectory is maybe not the best strategy for directing cell population. Instead, allowing individual cells switching around a predefined threshold seems to be a robust strategy leading to oscillating, but predictable, cell population behavior.

scholarly journals Reducing phenotypic and genotypic instabilities of microbial population during continuous cultivation based on stochastic switching dynamics

2021 ◽  
Author(s):  
Thai Minh Nguyen ◽  
Samuel Telek ◽  
Andrew Zicler ◽  
Juan Andres Martinez ◽  
Boris Zacchetti ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Genetic Instability ◽  
Microbial Population ◽  
Continuous Cultivation ◽  
Long Run ◽  
Switching Dynamics ◽  
On Line ◽  
Cultivation Process ◽  
Phenotypic Diversification ◽  
Stochastic Switching

AbstractPredicting the fate of a microbial population (i.e., growth, gene expression…) remains a challenge, especially when this population is exposed to very dynamic environmental conditions, such as those encountered during continuous cultivation processes. Indeed, the dynamic nature of continuous cultivation process implies the potential deviation of the microbial population involving genotypic and phenotypic diversification. This work has been focused on the induction of the arabinose operon in Escherichia coli as a model system. As a preliminary step, the GFP level triggered by an arabinose-inducible PBAD promoter has been tracked by flow cytometry in chemostat with glucose-arabinose co-feeding. Ampicillin was used as an “unstable” selective marker, allowing the simultaneous investigation of the effect of phenotypic diversification and genetic instability in continuous cultures. Under classical chemostat operation, the system was very unstable, with only a small fraction of cells (less than 10%) being able to accumulate GFP to a large extent, this fraction rapidly collapsing with time and going below 10% of the total population. On the long run, this phenotypic diversification was followed by an extensive loss of plasmid. In a second set of experiments, continuous cultivation was performed by adding either glucose or arabinose, based on the ability of individual cells for switching from low GFP to high GFP states, according to a technology called segregostat. In segregostat mode of cultivation, on-line flow cytometry analysis was used for adjusting the arabinose/glucose transitions based on the stochastic switching capabilities of the microbial population. This strategy allowed finding an appropriate arabinose pulsing frequency, leading to a prolonged maintenance of the induction level with limited impact of phenotypic diversification and genetic instability for more than 68 generations

SWITCHING DYNAMICS OF BISTABLE CdS ABSORPTIVE PLATELETS

1988 ◽  
Vol 49 (C2) ◽  
pp. C2-153-C2-156 ◽  
Author(s):  
J.-Y. BIGOT ◽  
A. DAUNOIS ◽  
N. CHERKAOUI EDDEQAQI ◽  
J. OBERLE ◽  
M. WEGENER ◽  
...  
Keyword(s):  
Switching Dynamics

A Study On Microbial Population Under The Effect Of Some Pesticides In Different Soil Orders And Moisture Contents

2018 ◽  
Vol 5 (14) ◽  
pp. 75-98
Author(s):  
Nashmeel Khudhur ◽  
Abdul-ghany Sarmamy
Keyword(s):  
Microbial Population ◽  
Moisture Contents

Ultrafast Switching Dynamics

2017 ◽  
Author(s):  
Olle Eriksson ◽  
Anders Bergman ◽  
Lars Bergqvist ◽  
Johan Hellsvik
Keyword(s):  
Lower Energy ◽  
Research Field ◽  
Optical Control ◽  
Magnetic Memory ◽  
Magnetic Media ◽  
Switching Dynamics ◽  
All Optical ◽  
Ultrafast Switching ◽  
Stored Information ◽  
Intense Research

The time-integrated amount of data and stored information, is doubled roughly every eighteen months, and since the majority of the worlds information is stored in magnetic media, the possibility to write and retrieve information in a magnetic material at ever greater speed and with lower energy consumption, has obvious benefits for our society. Hence the seemingly simple switching of a magnetic unit, a bit, is a crucial process which defines how efficiently information can be stored and retrieved from a magnetic memory. Of particular interest here are the concepts of ultrafast magnetism and all-optical control of magnetism which have in recent decades become the basis for an intense research field. The motivation is natural; the mechanisms behind these phenomena are far from trivial and the technological implications are huge.

Yield coefficients in dependence on milieu conditions and cell states II. Influence of perturbations on continuous cultivation of the yeastLodderomyces elongisporus on hydrocarbons

1981 ◽  
Vol 21 (8) ◽  
pp. 581-586 ◽  
Author(s):  
B. Heinritz ◽  
E. Stichel ◽  
T. Bley ◽  
G. Rogge ◽  
F. Glombitza
Keyword(s):  
Continuous Cultivation
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